FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应

doi:10.3969/j.issn.1004-616x.2002.03.002

癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (3): 144-146.doi: 10.3969/j.issn.1004-616x.2002.03.002

FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应

丁银润, 王晓燕, 汪　旭

云南师范大学生命科学学院遗传室,云南　昆明　650092

收稿日期:2001-07-24 修回日期:2001-09-30 出版日期:2002-07-30 发布日期:2002-07-30
通讯作者: 汪　旭

ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH

DING Yin-run, WANG Xiao-yan, WANG Xu

Genetic laboratory, School of Life Sciences, Yunnan Normal University, Kunming 650092, China

Received:2001-07-24 Revised:2001-09-30 Online:2002-07-30 Published:2002-07-30
Contact: WANG Xu

摘要/Abstract

摘要： 目的: 研究以荧光原位杂交(fluorescence in situ hybridization , FISH) 技术评价了中草药昆明山海棠根部水抽提物[ Tripterygium hypoglaucum ( Level) Hutch , THH]在小鼠骨髓细胞中的特异染色体不分离效应。方法:以受试物THH 腹腔注射昆明种雄性小白鼠,24 h 后取骨髓细胞常规制片,以bio-16-dUTP 标记的8 号染色体特异性着丝粒重复顺序探针进行FISH ,并以streptavidine-Cy3 与杂交信号结合。荧光显微镜下分析受试物处理动物后骨髓细胞8 号染色体分离情况。结果:在3 个受试剂量中,THH 具有与阳性对照秋水仙素类似的作用趋势,其所诱发的8 号染色体不分离频率均显著高于溶剂对照。结论:本研究证实了THH 为小鼠骨髓细胞8 号染色体异常分离的诱发因素。

关键词: 荧光原位杂交, 昆明山海棠, 小鼠骨髓细胞, 非整倍体

Abstract: Purpose : The water ext ract of a Chinese herb , Tri pterygium hypoglaucum ( Level ) Hutch ( THH)was assayed for its effects of aneuploidy induction in mouse bone marrow cells by means of fluorescence in situ hybridization (FISH) . Methods : Kunming species mice were injected with THH into the abdominal cavity and killed at 24 h after t reatment . The bone marrow cell slides were prepared by general methods. FISH was carried out with Bio2162dU TP2labbled chromosome 8 probe. The hybridization signals were detected by combining st reptavidine-Cy3. Results : In three dose groups THH-induced chromosome 8 aneuploidy and the aneuploidy frequencies of chromosome 8 were significantly higher than the solvent cont rol ( P < 0. 001～0. 05) . Conclusion : The result indicated that THH is an aneugen of chromosome 8 in mouse somatic cells.

Key words: fluorescence in situ hybridization, Tripterygium hypoglaucum (Lvel ) Hutch, mouse bone marrow cell, aneuploidy

中图分类号:

R99. 4

丁银润, 王晓燕, 汪　旭. FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应[J]. 癌变·畸变·突变, 2002, 14(3): 144-146.

DING Yin-run, WANG Xiao-yan, WANG Xu. ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(3): 144-146.

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FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应

ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH

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