癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (4): 220-222.doi: 10.3969/j.issn.1004-616x.2003.04.010

• 论著 • 上一篇    下一篇

顺铂、卡铂和双环铂对pBR322质粒DNA的断裂作用

马华智，刘正兴，王全军，阎长会，廖明阳   

  1. 军事医学科学院毒物药物研究所四室，北京　100850
  • 收稿日期:2003-05-29 修回日期:2003-07-27 出版日期:2003-10-30 发布日期:2003-10-30
  • 通讯作者: 廖明阳

THE BREAKING EFFECTS OF CISPLATIN, CARBOPLATIN AND DICYCLOPLATIN ON pBR322 PLASMID DNA

MA Hua-zhi , LIU Zheng-xing , WANG Quan-jun , et al   

  1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850 , China
  • Received:2003-05-29 Revised:2003-07-27 Online:2003-10-30 Published:2003-10-30
  • Contact: LIAO Ming-yang

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摘要： 目的：研究铂类抗癌药顺铂、卡铂和双环铂对pBR322质粒DNA的致断性。 方法：用琼脂糖凝胶电泳和凝胶成像分析方法 结果：顺铂、卡铂、双环铂均可诱发pBR322质粒DNA断裂，对质粒DNA的半数致断剂量分别为33.71 μmol/L、3.31 mmol/L和1.61 mmol/L。 结论：对pBR322质粒DNA的致断性强弱依次为：顺铂>双环铂>卡铂。

关键词: 顺铂, 卡铂, 双环铂, DNA断裂, 琼脂糖凝胶电泳, 凝胶成像分析

Abstract: Purpose:To study the breaking effects of Cisplatin, Carboplatin and Dicycloplatin on pBR322 plasmid DNA. Methods:Agrose gel electrophoresis and Gel image analysis was used. Results:All three platinum-species drugs could cause DNA break of pBR322 plasmid. The FC50 of Cisplatin, Carboplatin and Dicycloplatin were 33.71 μmol/L, 3.31 mmol/L, and 1.61 mmol/L , respectively. Conclusion:Breaking effects of tested drugs: Cisplatin > Dicycloplatin > Carboplatin.

Key words: cisplatin;carboplatin;dicycloplatin;DNA break;agrose gel electrophoresis, gel image analysis

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