巢式聚合酶链反应检测人类DNA修复基因：ERCC2/XPDexon 6突变

doi:10.3969/j.issn.1004-616x.2005.01.011

癌变·畸变·突变 ›› 2005, Vol. 17 ›› Issue (1): 33-35.doi: 10.3969/j.issn.1004-616x.2005.01.011

巢式聚合酶链反应检测人类DNA修复基因：ERCC2/XPDexon 6突变

郭　丽;马业罡;孙中芙;祁　荣;尹娇杨

沈阳医学院中心实验室，辽宁　沈阳　110034

收稿日期:2004-06-01 修回日期:2004-09-15 出版日期:2005-01-30 发布日期:2005-01-30
通讯作者: 尹娇杨

Detection of DNA Repair Gene: ERCC2/XPD exon 6 Mutation from Human by Nested Polymerase Chain Reaction (NPCR)

GUO Li; MA Ye-gang;SUN Zhong-fu; et al

The Center Laboratory, Shenyang Medical College,Shenyang 110034,China

Received:2004-06-01 Revised:2004-09-15 Online:2005-01-30 Published:2005-01-30
Contact: YIN Jiao-yang

摘要/Abstract

摘要： 背景与目的：探讨血样DNA来源困难及DNA产量低的情况下，获得理想的可供突变分析的PCR产物的方法。材料与方法：应用巢式聚合酶链反应(NPCR)-限制性片断长度多态(RFLP)技术检测了人类微量DNA样品的DNA修复基因： ERCC2/XPD exon 6的单核甘酸多态(SNP)。结果：该位点群体基因分型结果显示：自身平衡检验结果符合Hardy-Weinberg遗传平衡法则。结论：巢式PCR技术在扩增微量DNA、分析突变的实验中较一般单次扩增PCR技术更具有敏感性高，特异性强的优点。

关键词: 巢式聚合酶链反应, 微量DNA样品, DNA修复基因：ERCC2/XPD exon 6

Abstract: BACKGROUND ＆ AIM: To study a method which can get ideal products of PCR in the sample of low yield DNA for mutation analysis. MATERIAL AND METHODS: The nested polymerase chain reaction(NPCR)-restriction fragment length polymorphisms (RFLP) technique was used for mutation test of single nucleotide polymorphism of DNA repair gene: ERCC2/XPD exon 6 in some samples of low yield DNA.RESULTS: Genotyping results of population were in agreement with the expectations of Hardy-Weinberg Equilibrium. CONCLUSION: The sophisticated new“nested PCR” systems are significantly more sensitive and specific than other currently availble PCR technologies. 　

Key words: nested-polymerase chain reaction, low yield DNA, DNA repair gene: ERCC2/XPD exon 6

郭　丽, 马业罡, 孙中芙, 祁　荣, 尹娇杨. 巢式聚合酶链反应检测人类DNA修复基因：ERCC2/XPDexon 6突变[J]. 癌变·畸变·突变, 2005, 17(1): 33-35.

GUO Li, MA Ye-gang, SUN Zhong-fu, et al. Detection of DNA Repair Gene: ERCC2/XPD exon 6 Mutation from Human by Nested Polymerase Chain Reaction (NPCR)[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(1): 33-35.

巢式聚合酶链反应检测人类DNA修复基因：ERCC2/XPDexon 6突变

Detection of DNA Repair Gene: ERCC2/XPD exon 6 Mutation from Human by Nested Polymerase Chain Reaction (NPCR)

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