关键词: 重组人胰高血糖素类多肽, 致畸敏感期毒性, 致畸毒性

Abstract: BACKGROUND ＆ AIM： To observe whether there are embryonic and teratogenic toxicity on rats disposed by different dosages of recombinant human glucagon_like peptide(rhGLP) continuously during the period of organ formation. MATERIAL AND METHODS： The SD rats were used in this experiment. The pregnant rats were divided into five groups, three dosage groups (260,130 and 65 μg/kg), one positive and one negative control groups. There were more than 16 pregnant rats in every group. The treated groups and the negative control (normal physiological saline 1.0 ml/kg) group were injected continuously via hypodermic of back neck every day for ten days during the period of organ formation, while the positive control group was disposed via mouth by 10.0 ml/kg of aspirin. The numbers of luteal, nidation, alive_embryo, forepart fetal death, advanced stage fetal death were counted after the pregnant rats were sacrificed by disjointed cervical vertebrae at the twentieth day. After observed the sex, appearance and weight of embryonic rats, they were divided into two groups. In one group, the bone development was observed after ethanol fixing, potassium hydroxide melting, Alizarin Red staining transparencing. In another group, the viscera development was observed after Bouins solution fixing under and glycerin big_inspect_instrument. RESULTS： There were no obvious development abnormality in embryonic rats' appearances, bones and viscera in every disposed dosage groups. CONCLUSION： Recombinant glucagonant_like peptide has no embryonic and teratogenic toxicity under the dosage of 260 μg/kg.

Key words: recombinant human glucagon_like peptide, the period of sensitive teratogenesis, teratogenic toxicity