肺腺癌及其转移淋巴结的基因表达谱研究

doi:10.3969/j.issn.1004-616x.2006.02.007

癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (2): 105-107.doi: 10.3969/j.issn.1004-616x.2006.02.007

肺腺癌及其转移淋巴结的基因表达谱研究

张　薇;石玉枝;霍建民

哈尔滨医科大学第一临床医学院，黑龙江哈尔滨　150001

收稿日期:2005-07-13 修回日期:2005-09-30 出版日期:2006-03-30 发布日期:2006-03-30
通讯作者: 石玉枝

Gene Expression Profile of Pulmonary Adenocarcinoma and Its Metastatic Lymph Nodes

ZHANG Wei;SHI Yu-zhi;HUO Jian-min

The First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China

Received:2005-07-13 Revised:2005-09-30 Online:2006-03-30 Published:2006-03-30
Contact: SHI Yu-zhi

摘要/Abstract

摘要： 背景与目的：利用基因芯片技术研究肺腺癌组织及其转移淋巴结组织基因表达差异。材料与方法：分别抽取3例肺腺癌组织、转移淋巴结组织的总RNA，通过逆转录合成cDNA，以两种荧光cy5和cy3标记，制备cDNA探针，并与含有1 152个人类全长基因的cDNA基因芯片进行杂交。以ScanArray4000荧光扫描仪扫描芯片上两种荧光信号，用计算机处理和分析。结果： 3例肺腺癌组织和转移淋巴结组织标本中，8个基因有共同表达差异，其中上调基因5个，下调基因3个。结论：肺腺癌组织和转移淋巴结组织之间存在着基因表达差异，两者比较共表达差异的8个基因可能与肺腺癌的发展、转移有关。

关键词: 肺腺癌, 转移淋巴结, 基因表达谱

Abstract: BACKGROUND ＆ AIM: To investigate the differences of gene expression profile among the pulmonary adenocarcinoma and metastatic lymph node tissues by means of cDNA microarray. MATERIAL AND METHODS: Total RNA was extracted from pulmonary adenocarcinoma and metastatic lymph node tissues in order to get purified mRNA which was retro-transcribed into cDNA. As the probes, the cDNA was labeled with Cy5 and Cy3 fluorescence, then hybridized with the whole human gene chip. The signal image was obtained by Scan Array 4000 fluorescence scanner and analyzed with computer software. RESULTS: 8 genes in 3 subjects with pulmonary adenocarcinoma and metastatic lymph node tissues were identified with common differences in gene expression level, including 5 up-regulated and 3 down-regulated genes. CONCLUSION: The expression levels of 8 genes found both in pulmonary adenocarcinoma and metastatic lymph node tissues were probably related to the development and metastasis of lung cancer.

Key words: pulmonary adenocarcinoma, metastatic lymph nodes, gene expression profile

中图分类号:

Q132.7
Q754

张　薇, 石玉枝, 霍建民. 肺腺癌及其转移淋巴结的基因表达谱研究[J]. 癌变·畸变·突变, 2006, 18(2): 105-107.

ZHANG Wei, SHI Yu-zhi, HUO Jian-min. Gene Expression Profile of Pulmonary Adenocarcinoma and Its Metastatic Lymph Nodes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(2): 105-107.

[1]	曾卓颖, 吴德生, 鲁晶晶, 廖慧, 赖洪飘, 袁建辉, 胡章立. 雌激素受体α介导氧化磷酸化通路在肺癌发生中的作用[J]. 癌变·畸变·突变, 2021, 33(4): 262-268.
[2]	杜强, 姚义勇, 曾刚. 基于TCGA数据库分析ASPM在肺腺癌中的表达及临床意义[J]. 癌变·畸变·突变, 2020, 32(6): 457-463.
[3]	马阳, 吴娟, 纪晓坤, 王蕊, 杜芸, 王珩, 郭晓, 张艳. 沉默表皮生长因子受体的表达对肺腺癌A549细胞自噬的影响[J]. 癌变·畸变·突变, 2019, 31(5): 347-351,358.
[4]	李雨轩, 李凯, 张彤彤, 郭元彪, 殷俊, 包勇. 热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究[J]. 癌变·畸变·突变, 2017, 29(5): 358-363.
[5]	孙　静, 张　丹, 朱庆均. 益气养阴方抑制肿瘤转移及对肿瘤相关基因表达谱的影响[J]. 癌变·畸变·突变, 2009, 21(5): 348-352.
[6]	黄素英(综述), 陈　华(审校). 基因芯片在胃癌基因表达谱中的应用[J]. 癌变·畸变·突变, 2008, 20(6): 493-495.
[7]	胡迎春/ 霍艳英/ 钟科军/ 杨陟华/ 徐国蕊/ 潘秀颉/ 吴德昌/ 朱茂祥. 卷烟烟气诱发永生化人支气管上皮细胞恶性转化不同阶段的基因表达谱研究[J]. 癌变·畸变·突变, 2008, 20(1): 44-049.
[8]	胡　冉;敖　琳;高利宏;曹　佳. 十三种化合物诱导的小鼠原代培养肝细胞基因表达谱的聚类分析[J]. , 2006, 18(2): 149-152.
[9]	郭苗莉;朱江波;陈蓉芳;张天宝. 应用基因表达谱芯片研究MNNG诱致小鼠胚胎畸形肢体基因表达的变化[J]. , 2005, 17(2): 71-75.
[10]	张　薇; 李殿俊;石玉枝. 胚胎肺和正常肺组织基因表达谱差异[J]. , 2004, 16(2): 92-93.
[11]	李　煜;杨素霞;周淑珍;刘国廉. 保肺消瘤胶囊制剂抗肺癌的药效学研究[J]. , 2004, 16(1): 33-35.

肺腺癌及其转移淋巴结的基因表达谱研究

Gene Expression Profile of Pulmonary Adenocarcinoma and Its Metastatic Lymph Nodes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价