关键词: 酵母单杂交, 食管癌, cDNA文库

Abstract: BACKGROUND＆AIM： This study was to screen an esophageal cancer cell line (SHEEC) cDNA library for the cDNA that expressed a protein that can specifically bind the NF-kappa B element, for further understanding the expression and regulation mechanism of the NGAL(neutrophil gelatinase-associated lipocalin) gene, and the course for the over-expression of the NGAL gene in the esophageal epithelial squamous carcinoma. MATERAL AND METHODS：A tandem three oligo-nucleotides of NF-kappa B was employed as a bait in a yeast one hybrid system to screen the cDNA library of the human esophageal carcinoma cell line (SHEEC). The cDNA clones obtained were sequenced and analyzed with bioinformatics tools. RESULTS： Among the thirty clones obtained, four types of clones could encode proteins, two clones were hypothetical proteins, two types of clones were mitochondrial genes, and three types of clones were non-encoding sequences. CONCLUSION： Using the yeast one hybrid system to screen the cDNA library for the NF-kappa B core element is a preliminary work that needs confirmation by further study.

Key words: yeast one hybrid, esophageal carcinoma, cDNA libriary