关键词: 富硒益生菌, 急性毒性, Ames试验, 微核试验, 精子畸形试验

Abstract: BACKGROUND ＆ AIM: To study the acute toxicity and genotoxicity of selenium-enriched probiotics. MATERIALS AND METHODS: Acute toxicity test of mice,Ames test,micronucleus test of bone marrow PCE cell in mice, sperm abnormality test of mice were used. RESULTS: The oral acute toxicity study revealed that the LD50 of selenium-enriched probiotics was 18.49 g/kg in mice. The results of genotoxicity tests were all negative, including Ames test, micronucleus test and sperm shape abnormality test. CONCLUSION: Selenium-enriched probiotics showed certain toxicity but no genotoxicity in this study. Thus,selenium-enriched probiotics was safe at the daily-recommended dose(50 mg/person/day) and has the potential for development into functional foods.

Key words: selenium-enriched probiotics, acute toxicity, Ames test, micronucleus test, sperm abnormality test