高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响

doi:10.3969/j.issn.1004-616x.2008.01.004

癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (1): 15-018.doi: 10.3969/j.issn.1004-616x.2008.01.004

高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响

邢秀梅1,2，张坚松1，刘建军2，席仁荣2，黄海燕2

1. 湖南师范大学医学院应用生理研究室，湖南　长沙　410006；2. 深圳市疾病预防控制中心，广东　深圳　518020

收稿日期:2007-07-26 修回日期:2007-09-11 出版日期:2008-01-30 发布日期:2008-01-30
通讯作者: 张坚松，刘建军

Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene

XING Xiu-mei1,2，ZHANG Jian-song1,，LIU Jian-jun2, XI Ren-rong2，HUANG Hai-yan2

1.Research Department of Applied Physiology, Medical College, Hunan Normal University, Changsha 410006;2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, Guangdong, China

Received:2007-07-26 Revised:2007-09-11 Online:2008-01-30 Published:2008-01-30
Contact: ZHANG Jian-song, LIU Jian-jun

摘要/Abstract

摘要： 背景与目的：观察高剂量三氯乙烯( trichloroethylene,TCE )对L-02肝细胞中Rho GDIα基因在 mRNA及蛋白水平表达的影响。材料与方法：以β-actin为内参，采用蛋白印迹 (western blot) 和实时荧光定量RT- PCR技术对分别40 μmol/L TCE(实验组)和DMSO(溶剂对照组)处理的正常L-02肝细胞中的Rho GDIα蛋白和mRNA水平进行检测。结果：高剂量TCE 处理后Rho GDIα mRNA表达量显著下降而蛋白含量明显升高。结论： Rho GDIα基因表达在高剂量TCE处理后发生了特异性改变，为揭示TCE 对机体的损伤作用机制提供了重要线索。

关键词: 三氯乙烯, Rho GDIα, western blot, 实时定量RT- PCR

Abstract: BACKGROUND AND AIM: To study the effect of trichloroethylene(TCE) on the expression change of Rho GDIα gene at both mRNA and protein levels. MATERIALS AND METHODS: Western blot and real time quantitative RT-PCR were employed to detect the protein and mRNA levels of Rho GDIα in L-02 liver cells ,which were treated with DMSO and high concentrations of TCE. β-actin was selected as internal standard. RESULTS: The protein level of Rho GDIα was significantly up-regulated while its mRNA was significantly reduced. CONCLUSION: The Rho GDIα gene expression level showed specific changes after treatment with high concentration of TCE, providing important clues to the mechanism of TCE-induced damage in humans.

Key words: trichloroethylene, Rho GDIα, western blot, real time quantitative RT-PCR

邢秀梅, 张坚松, 刘建军, 席仁荣, 黄海燕. 高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响[J]. 癌变·畸变·突变, 2008, 20(1): 15-018.

XING Xiu-mei, ZHANG Jian-song, , LIU Jian-jun, XI Ren-rong, HUANG Hai-yan. Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(1): 15-018.

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高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响

Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene

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