人精子携带的HBs和HBc基因在早期胚胎细胞中的蛋白表达

doi:10.3969/j.issn.1004-616x.2008.03.005

癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (3): 185-188.doi: 10.3969/j.issn.1004-616x.2008.03.005

人精子携带的HBs和HBc基因在早期胚胎细胞中的蛋白表达

张秋菊黄天华 谢庆东谭小方刘戈飞陈德宇周小玲

汕头大学医学院生殖医学研究中心，广东　汕头　515041

收稿日期:2008-01-29 修回日期:2008-02-28 出版日期:2008-05-30 发布日期:2008-05-30
通讯作者: 黄天华

Protein Expression of Human Spermatozoa-mediated HBs Gene and HBc Gene in Early Embryonic Cells

ZHANG Qiu-ju, HUANG Tian-hua, XIE Qing-dong ,TAN Xiao-fang, LIU Ge-fei, CHEN De-yu, ZHOU Xiao-ling

Research Center for Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong,China

Received:2008-01-29 Revised:2008-02-28 Online:2008-05-30 Published:2008-05-30
Contact: HUANG Tian-hua

摘要/Abstract

摘要： 背景与目的：探讨由人精子带入受精卵中的HBs和HBc基因能否在早期胚胎细胞中进行蛋白表达。材料与方法：人精子经重组质粒pIRES2-EGFP-HBV转染后，与金黄地鼠去透明带卵母细胞离体受精，选择带绿色荧光的2-细胞胚，用免疫荧光技术检测HBs和HBc基因在胚胎细胞中的蛋白表达，用ELISA方法分别对HBsAg和HBcAg进行半定量和定性分析。结果：在带绿色荧光的2-细胞胚中免疫荧光检测可见清楚的HBsAg和HBcAg阳性信号；ELISA结果表明，单个2-细胞胚内HBsAg的量小于0.064 ng/ml，对HBcAg的检测得到阳性结果。结论：人精子携带到卵内的HBV基因可在早期胚胎细胞中表达表面抗原和核心抗原。

关键词: HBV基因, 人精子, 胚胎细胞, 蛋白表达

Abstract: BACKGROUND AND AIM: To study the protein expression of HBs and HBc genes brought into embryos via human spermatozoa. MATERIALS AND METHODS: Human spermatozoa were transfected with recombinant plasmid pIRES2-EGFP-HBV and were then fertilized with zona pellucida-free golden hamster ova. The 2-cell embryos with and without green fluorescence were collected by immunofluorescence assay and ELISA analysis, respectively. RESULTS: The immunofluorescence assay showed that no HBsAg- and HBcAg- positive signal was observed in 2-cell embryos without green fluorescence and the clear HBsAg- and HBcAg- positive signals were detected in ones with green fluorescence. ELISA analysis showed that the amount of HBsAg in a single 2-cell embryo was about 0.064 ng/ml and the positive result for HBcAg was detected. CONCLUSION: Human sperm-mediated HBs and HBc genes are able to express their proteins in early embryonic cells.

Key words: HBV gene, human spermatozoa, embryonic cells, protein expression

张秋菊黄天华 谢庆东谭小方刘戈飞陈德宇周小玲. 人精子携带的HBs和HBc基因在早期胚胎细胞中的蛋白表达[J]. 癌变·畸变·突变, 2008, 20(3): 185-188.

ZHANG Qiu-ju, HUANG Tian-hua, XIE Qing-dong , TAN Xiao-fang, LIU Ge-fei, CHEN De-yu, ZHOU Xiao-ling. Protein Expression of Human Spermatozoa-mediated HBs Gene and HBc Gene in Early Embryonic Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(3): 185-188.

[1]	王丹, 耿兴超, 李波, 王雪, 文海若. 体外细胞转化试验的研究与应用进展[J]. 癌变·畸变·突变, 2018, 30(4): 310-314.
[2]	程琳, 孙平楠, 谢庆东, 周小玲. 预扩增qPCR方法检测少量小鼠早期胚胎细胞中DNA甲基化相关基因的表达[J]. 癌变·畸变·突变, 2016, 28(1): 51-55.
[3]	周小玲, 孙平楠, 康祥锦, 刘冬玲, 谢庆东. 乙肝病毒表面蛋白对人精子活力及线粒体膜电位的影响[J]. 癌变·畸变·突变, 2010, 22(2): 100-103.
[4]	王丽, 张学红, 刘金荣. 人精子穿去透明带金黄地鼠卵实验和精子DNA完整性检测在体外受精-胚胎移植中的应用[J]. 癌变·畸变·突变, 2010, 22(1): 43-0047.
[5]	孙平楠, 周小玲, 黄天华, 谢庆东, 陈惠芳, 康祥锦. FITC标记无唾液酸胎球蛋白对人精子去唾液酸糖蛋白受体的定位[J]. 癌变·畸变·突变, 2009, 21(2): 132-134.
[6]	刘　婷1;王新允2;朱丛中2;李　艳2;王爱香2;孙翠云2;赵凤云2. 肺癌组织中MRP-1和FAK蛋白的表达及其临床意义的探讨[J]. , 2006, 18(2): 96-099.
[7]	方小武　黄天华　黄建民　刘鸿禧　陶学训　余云香　庄天罡. 人精子诱变检测中新体外代谢活化系统的研究[J]. , 1999, 11(3): 116-118.
[8]	黄建民1 　黄天华1 　邱焕英2 　邱杰文3 　方小武1 　陈晓红1 　庄天罡1 　蔡树深1 　王永华2 　刘鸿禧1 　杨风霞2 　刘卫东2. 乙型肝炎病毒对精子染色体的影响[J]. , 1999, 11(2): 72-074.
[9]	郑履康　邓丽霞　张　桥. 荧光原位杂交(FISH) 检测人精子染色体非整倍体率方法的研究[J]. , 1998, 10(5): 267-271.
[10]	傅宝华1 　吕玉民1 　刘金荣2 　陈玉浩1 　任春仙2 　赵凤玲1. 60Coγ射线全身照射与离体照射诱发人精子染色体畸变研究[J]. , 1998, 10(1): 31-034.
[11]	余云香1 　黄天华2 　黄建民2　刘鸿禧2　方小武2. DNA诱发损伤的修复与染色体畸变的研究——咖啡因对人精子染色体的影响[J]. , 1997, 09(1): 13-19.
[12]	高晓平，李丹，吴无畏，吴德光. 地鼠血清培养异合卵制备人精子染色体的研究[J]. , 1996, 08(2): 117-121.

人精子携带的HBs和HBc基因在早期胚胎细胞中的蛋白表达

Protein Expression of Human Spermatozoa-mediated HBs Gene and HBc Gene in Early Embryonic Cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

本文评价