黄芪注射液对高糖所致血管内皮细胞损伤的保护作用

doi:10.3969/j.issn.1004-616x.2008.04.012

癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (4): 295-298.doi: 10.3969/j.issn.1004-616x.2008.04.012

黄芪注射液对高糖所致血管内皮细胞损伤的保护作用

范　可1 /陈培红2/ 吴　青3

1．华中科技大学同济医学院附属梨园医院,湖北　武汉　430000； 2.武汉市中医院,湖北　武汉430014；3.华中科技大学同济医学院附属协和医院，湖北　武汉　430022

收稿日期:2007-09-24 修回日期:2008-03-09 出版日期:2008-07-30 发布日期:2008-07-30

Protective Effects of Huangqi on Vascular Endothelial Cells Exposed to High Gluose

FAN Ke1,CHEN Pei_hong2,WU Qing3

1. Liyuan Hospital,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430000;2. Wuhan Hospital of Tradional Chinese Medicine, Wuhan 430014; 3.Institute of Hematotogy Union Hospital, Tongji Medical Colllege of Huazhong University of Science and Technology, Wuhan 430022,China

Received:2007-09-24 Revised:2008-03-09 Online:2008-07-30 Published:2008-07-30

摘要/Abstract

摘要： 背景与目的：研究黄芪注射液对50％葡萄糖所诱导的血管内皮细胞损伤的保护作用。材料与方法: 采用血管内皮细胞VEC_304,分为正常对照组、葡萄糖损伤对照组和加入3个不同黄芪浓度的实验组(100 mg/ml、200 mg/ml、300 mg/ml), 各组细胞孵育24 h后 ,除正常对照组外其余各组加入50％葡萄糖(终浓度为30 mg/L)诱导损伤，继续培养4 h。在倒置相差显微镜下观察细胞形态学变化并采用MTT光吸收法检测细胞活力、采用考马斯亮蓝法检测蛋白质含量, 用RT_PCR法和免疫细胞化学方法检测血管内皮生长因子(VEGF mRNA)及蛋白的表达。结果: 正常对照组及黄芪注射液各剂量组细胞活力、蛋白质含量均高于50％葡萄糖损伤对照组，其差异均具有统计学意义(P均<0.05)。显示高糖明显抑制VEGF的活性和表达,而黄芪则具有明显的对高糖损伤的修复作用。结论：黄芪注射液可以减轻高糖对内皮细胞的损伤,具有一定的保护作用。

关键词: 黄芪注射液, 高糖, VEC_304 细胞

Abstract: BACKGROUND AND AIM: To evaluate the protective effects of Huangqi on the damage of high glucose on vascular endothelial cell(VEC_304)in vitro． MATERIALS AND METHODS: The model of human umbilical vein endothelial cells (VEC_304) damage was induced by 50％ glucose for 4 h.The cells were pretreated with three concentrations of Huangqi solution for 24 h,before they were exposed to glucose. MTT and protein levels in cells were determined after 4 h. The expression of VEGF was investigated by RT_PCR and immunohistochemistry methods. RESULTS: MTT and protein levels in cells of the normal control group amd Huangqi_treated groups were higher than in glucose_damaged group without huangqi(P<0.05). The expression of VEGF was inhibited by glucose. CONCLUSION: Huangqi could protect the damages of VEC_304 endothelial cells induced by high glucose.

Key words: Huangqi, glucose, vascular endothelial cells 304

范　可 /陈培红/ 吴　青. 黄芪注射液对高糖所致血管内皮细胞损伤的保护作用[J]. 癌变·畸变·突变, 2008, 20(4): 295-298.

FAN Ke, CHEN Pei_hong, WU Qing. Protective Effects of Huangqi on Vascular Endothelial Cells Exposed to High Gluose[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(4): 295-298.

黄芪注射液对高糖所致血管内皮细胞损伤的保护作用

Protective Effects of Huangqi on Vascular Endothelial Cells Exposed to High Gluose

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