癌变·畸变·突变 ›› 2009, Vol. 21 ›› Issue (2): 145-148.doi: 10.3969/j.issn.1004-616x.2009.02.016

• 肿瘤防治 • 上一篇    下一篇

循环肿瘤细胞p53基因表达和端粒酶活性在大肠癌临床病理学中的意义

周建农1,马国建2,何晓松2,沈宗丽2,薛开先2,   

  1. 1.江苏省肿瘤医院普外科, 江苏 南京 210009; 2.江苏省肿瘤防治研究所遗传学
  • 收稿日期:2008-05-12 修回日期:2008-11-25 出版日期:2009-03-30 发布日期:2009-03-30

Clinicopathological Significance of p53 Gene Expression and Telomerase Activity in

ZHOU Jian-nong1, MA Guo-jian2,HE Xiao-song2,SHEN Zong-li2,XUE Kai-xian2   

  1. 1. Department of General Surgery, Jiangsu Cancer Hospital, Nanjing 210009; 2. Laboratory of Genetics, Jiangsu Institute for Cancer Research, Nanjing 210009,Jiangsu,China
  • Received:2008-05-12 Revised:2008-11-25 Online:2009-03-30 Published:2009-03-30

摘要: 背景与目的: 探讨大肠癌患者循环肿瘤细胞p53基因的表达和端粒酶活性的变化及其临床病理学意义。 材料与方法: 113例大肠癌患者、13例良性大肠肿瘤患者术前的外周血和28例健康供血员的外周血,用分层液分离含有循环肿瘤细胞的单个核细胞,应用流式细胞仪和TRAP-ELISA法,分别检测p53基因表达和端粒酶活性。 结果: 在105例有信息的大肠癌患者中,p53基因表达和端粒酶活性的阳性率分别为74.3%(78/105) 和 61.0%(64/105)。异常的p53基因表达和端粒酶活性水平均与大肠癌患者的淋巴结转移显著相关(P<0.05),两者的联合检测提高了对大肠癌淋巴结转移的预测水平。p53基因异常表达还与大肠癌细胞分化程度显著相关(P=0.003)。 结论: 循环肿瘤细胞异常的p53基因表达和端粒酶活性是大肠癌的恶性生物学标志,两者密切相关,联合检测可提高对淋巴结转移状态的预测水平。

关键词: 大肠癌, p53基因, 端粒酶, 转移, 循环肿瘤细胞

Abstract: BACKGROUND AND AIM: To study the clinicopathological significance of p53 gene expression and telomerase activity in circulating tumor cells of patients with colorectal carcinoma. MATERIALS AND METHODS: Mononuclear cells containing circulating tumor cells were isolated by Ficoll-Hypaque gradient centrifugation in peripheral blood from 113 preoperative patients with colorecatal carcinomas,13 patients with benign colorectal disease, and 28 healthy volunteers. FCM and TRAP-ELASA were used to evaluate p53 gene expression and telomerase activity,respectively. Experimental data were analyzed by SPSS 11.0 software and χ2 test. RESULTS: Among 105 informative patients with colorectal carcinoma, positive rate of p53 expression and telomerse activity were 74.3%(78/105) and 61.0%(64/105),respectively. Aberrant expression levels of both p53 gene and telomerase in patients with colorectal cancer were significantly correlated with lymph node metastasis (P<0.05) and their simultaneous measurement could raise the prediction accuracy of metastasis. Expression of p53 gene was also significantly correlated with differentiation degree of colorectal carcinoma (P=0.003). CONCLUSION: Aberrant p53 gene expression and telomerase activity in circulating tumor cells might be malignant biomarkers. Both were closely related and could predict nodal involvement.

Key words: colorectal carcinoma, p53 gene, telomerase, metastasis, circulating tumor cells