脂质体介导pcDNA3.1-IDO转染hepG2细胞的初步研究

doi:10.3969/j.issn.1004-616x.2010.03.005

癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (3): 179-181.doi: 10.3969/j.issn.1004-616x.2010.03.005

脂质体介导pcDNA3.1-IDO转染hepG2细胞的初步研究

冯惠枝1;王琦2

1. 山西医科大学，山西太原 030001； 2. 山西医科大学附属第二临床医学院消化内科，山西太原 030001

收稿日期:2009-12-29 修回日期:2010-03-06 出版日期:2010-05-30 发布日期:2010-05-30
通讯作者: 王琦

Preliminary study of human hepatoma carcinoma cell line hepG2 transfected with plasmid pcDNA3.1-IDO by lipofectamine

FENG Hui-zhi1;WANG Qi2

1.Shanxi Medical University,Taiyuan 030001; 2.Department of Gastroenterology,Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China

Received:2009-12-29 Revised:2010-03-06 Online:2010-05-30 Published:2010-05-30
Contact: WANG Qi

摘要/Abstract

摘要： 目的：建立脂质体介导pcDNA3.1-IDO转染人肝癌细胞株hepG2细胞的转染方法。方法：在大肠杆菌中扩增pcDNA3.1-IDO质粒，通过lipofectamineTM2000转染试剂将pcDNA3.1-IDO转入人肝癌细胞hepG2，同时设置空质粒转染组(pcDNA3.1- hepG2细胞)和空白对照组(hepG2细胞)。分别应用RT-PCR和Western blot方法检测吲哚胺2,3-双加氧酶(IDO)基因及IDO蛋白在hepG2细胞中的表达。结果：经RT-PCR和Western blot检测证实空质粒转染组和空白对照组hepG2细胞无IDO基因及蛋白的表达，而重组质粒组的hepG2细胞均表达IDO基因和蛋白。结论：通过脂质体介导成功地将pcDNA3.1-IDO转染入肝癌细胞株hepG2细胞。这为研究IDO基因奠定了基础。

关键词: 基因转染, 人类吲哚胺2, 3-双加氧酶, hepG2细胞

Abstract: OBJECTIVE: To determine the transfection expression of plasmid pcDNA3.1-IDO in human hepatoma carcinoma cell line hepG2, and to establish a transfection method of hepG2. METHODS: The plasmid pcDNA3.1-IDO was amplified in Escherichia coli. The cultured hepG2 cells were transfected with pcDNA3.1-IDO by lipofectamineTM2000 reagent. The hepG2 cells and hepG2 cells trancfected with blank plasmid pcDNA3.1(pcDNA3.1- hepG2 cell) were used as control group. The transient expression of IDO in hepG2 cells transfected with recombinant plasmid was determined by RT-PCR and Western blot. RESULTS: IDO gene and protein were expressed transiently in hepG2 cells transfected with recombinant plasmid shown by RT-PCR and Western blot,respectively. CONCLUSION: The IDO gene was successfully transfected into human hepatoma carcinoma cell line hepG2 by means of lipofectamineTM2000 reagent, which provides the basis for further studies of IDO gene.

Key words: gene transfection, human indoleamine 2, 3-dioxygenase, hepG2 cell

中图分类号:

Q813

冯惠枝, 王琦. 脂质体介导pcDNA3.1-IDO转染hepG2细胞的初步研究[J]. 癌变·畸变·突变, 2010, 22(3): 179-181.

FENG Hui-zhi, WANG Qi. Preliminary study of human hepatoma carcinoma cell line hepG2 transfected with plasmid pcDNA3.1-IDO by lipofectamine[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(3): 179-181.

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脂质体介导pcDNA3.1-IDO转染hepG2细胞的初步研究

Preliminary study of human hepatoma carcinoma cell line hepG2 transfected with plasmid pcDNA3.1-IDO by lipofectamine

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