关键词: 维生素E琥珀酸酯, 活性氧, Ca2＋, 氧化损伤

Abstract: OBJECTIVE: To study the apoptosis effect of SGC-7901 cells induced by Vitamin E succinate(VES), and explore the involvement and mechanisms of oxidative damage. METHODS: After treatment by 0, 5, 10, 20 μg/ml VES for 24 h, methods of flow cytometry, confocal laser scanning microscopy and western blot were used to measure the mitochondrial oxidative damage and apoptosis induced by VES of SGC-7901 cells. The corresponding detection kits were used to detect the reactive oxygen species and Ca2＋ content. At the same time, SGC-7901 cells were treated with 1 mmol/L of mannitol for 2 h, and then 20 μg/ml VES for 24 h, and the above detection methods were used. RESULTS: With the increasing concentration of VES, apoptosis showed an increasing trend. Compared with the negative control group, ΔΨm of cells in 10, 20 μg/ml VES groups were significantly decreased, while the active oxygen content and the level of Ca2＋ significantly was increased. 1 mmol/L of mannitol could impact the cells of oxygen and Ca2＋ contents significantly, while significantly reduce mitochondrial oxidative damage and apoptosis caused by VES. CONCLUSION: VES could significantly increase SGC-7901 apoptosis, and with increasing doses of VES, the apoptosis rate was higher. Oxidative damage caused by raised active oxygen and Ca2＋ content increasing and ΔΨm depletion in cells may be one of the mechanisms of apoptosis induced by VES.

Key words: Vitamin E succinate, active oxygens, Ca2＋, oxidative damage