癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (3): 221-225.doi: 10.3969/j.issn.1004-616x.2010.03.015

• 检测研究 • 上一篇    下一篇

NJY冻干粉对生理机能的影响及其免疫调节和抗肿瘤作用

展丽娟1;王爱平1;侯晓明1;林晓琪1;李偃2   

  1. 1．中国医学科学院中国协和医科大学新药安全评价中心，北京 100050；2．西安新星医药研究所，陕西 西安 710065
  • 收稿日期:2009-10-20 修回日期:2010-03-19 出版日期:2010-05-30 发布日期:2010-05-30
  • 通讯作者: 王爱平

The physical tolerance, immune-regulating and antineoplastic effects of NJY lyophilized powder

ZHAN Li-juan1; WANG Ai-ping1;HOU Xiao-ming1;LIN Xiao-qi1;LI Yan2   

  1. 1. Institute of Materia Medica, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100050; 2. Xi'an New Star Research Institute of Chinese Medicine, Xi'an 710065,Shaanxi, China
  • Received:2009-10-20 Revised:2010-03-19 Online:2010-05-30 Published:2010-05-30
  • Contact: WANG Ai-ping

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摘要： 目的： 研究NJY冻干粉对机体体能的影响及其免疫调节作用和抗肿瘤作用。 方法： 分别按NJY冻干粉16、32、64 mg/kg剂量给ICR种小鼠灌胃给药，进行负重游泳实验、耐缺氧实验、迟发超敏反应实验和巨噬细胞吞噬功能实验，观察NJY冻干粉对小鼠生理机能及免疫功能的影响。用NJY冻干粉给S180荷瘤昆明小鼠灌胃，观察对肉瘤生长的影响。另用NJY冻干粉给S180荷瘤昆明小鼠灌胃，并给小鼠腹腔一次性注射环磷酰胺，观察NJY冻干粉对环磷酰胺化疗作用的影响，以及NJY冻干粉对移植性S180腹水瘤小鼠存活时间的影响。 结果： NJY冻干粉16 mg/kg和64 mg/kg组小鼠抗疲劳能力较对照组明显提高(P<0.05)； 32 mg/kg和64 mg/kg组小鼠耐缺氧能力较对照组明显提高(P<0.05)；NJY冻干粉32 mg/kg和64 mg/kg组小鼠耳肿胀率较对照组明显增加(P<0.05)；NJY冻干粉给药组巨噬细胞吞噬指数与对照组比较差异无统计学意义(P>0.05)。NJY冻干粉给药组小鼠S180型肉瘤抑瘤率与对照组比较差异无统计学意义(P>0.05)；NJY冻干粉给药组腹水瘤小鼠生命延长率与对照组比较差异无统计学意义(P>0.05)；环磷酰胺60mg/kg与NJY冻干粉合用后，抑瘤率较环磷酰胺对照组显著提高(P<0.05)。 结论： NJY冻干粉具有免疫增强作用, 可以提高小鼠的抗疲劳能力和耐缺氧能力, 能增强环磷酰胺的抗肿瘤作用。

关键词: NJY冻干粉, 生理机能, 免疫调节, 抗肿瘤作用

Abstract: OBJECTIVE: To study the effects of NJY lyophilized powder on physical tolerance and its immune- regulating, antineoplastic effects. METHODS: Loaded-swimming test, hypoxia tolerance test, delayed hypersensitive reaction(DHR) experiment, and macrophage phagocytosis experiments were conducted to investigate the effects of NJY on immune function. By transplanting S180 (1.0×107/ml) sarcoma at the axilla of Kunming mice, the inhibitory effect of NJY on its growth was observed, promotion of the antineoplastic effect of cyclophosphamide, and the possibility of extending mice survival were examined. RESULTS: NJY 16 mg/kg and 64 mg/kg exerted an obvious anti-fatigue effect on mice, with significant difference(P<0.05) from the control group; the survival time of mice with hypoxia in NJY 32 mg/kg group and 64 mg/kg group was significantly increased, with significant difference(P<0.05) from the control group. DHR: swelling extent of mice ear in NJY 32 mg/kg and 64 mg/kg groups was significantly increased compared with the model control group, with significant difference(P<0.05). Macrophage phagocytosis experiment: the phagocytic index of NJY dose groups had no significant difference(P>0.05). The inhibiton rates of NJY dose groups had no significant difference(P>0.05), compared with control group. The life extension rates of NJY dose groups were not prolonged, without significant difference(P>0.05). Combined administration of NJY with cyclophosphamide significantly increased the antineoplastic effect of cyclophosphamide. The inhibition rates were significantly increased compared with the control group after combined administration of cyclophosphamide 60 mg/kg and NJY 16, 32 and 64 mg/kg dose, with significant difference (P<0.05).CONCLUSION:NJY lyophilized powder has immune enhancing effect; it can enhance the anti-fatigue ability of mice and anti-anoxia capacity; markedly enhanced anti-tumor effect of cyclophosphamide.

Key words: NJY lyophilized powder, physical tolerance, immune regulation, antineoplastic effect

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