癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (6): 455-457.doi: 10.3969/j.issn.1004-616x.2010.06.011

• 论著 • 上一篇    下一篇

人免疫缺陷病毒_1 gag基因在人成熟精子中的表达

王 典1,2康祥锦1,谢庆东1,黄天华1   

  1. 1. 汕头大学医学院生殖医学研究中心; 2. 汕头大学医学院法医学教研室, 广东汕头 515041
  • 收稿日期:2010-09-07 修回日期:2010-09-21 出版日期:2010-11-30 发布日期:2010-11-30
  • 通讯作者: 王 典

The expression of human immunodeficiency virus _1 gag gene in mature human spermatozoa

WANG Dian1,2, KANG Xiang-jin1, XIE Qing-dong1, HUANG Tian-hua1,   

  1. 1. Research Center for Reproductive Medicine; 2. Forensic Medicine Department, Shantou University Medical College, Shantou 515041,Guangdong, China
  • Received:2010-09-07 Revised:2010-09-21 Online:2010-11-30 Published:2010-11-30
  • Contact: WANG Dian1

摘要: 目的: 探讨人免疫缺陷病毒_1(HIV_1) gag基因在人成熟精子中实现mRNA水平表达的可能性。 方法: 构建含HIV_1 gag 基因DNA的质粒,转染入人成熟精子,培养24 h后用RT_PCR法检测精子gag mRNA的表达。 结果: 在转染质粒后的人成熟精子中检测到HIV_1 gag mRNA的表达。 结论: HIV基因可在人成熟精子中实现mRNA水平的表达。

关键词: 人免疫缺陷病毒_1, 人成熟精子, mRNA

Abstract: OBJECTIVE: To explore the possibility of HIV gag gene mRNA expression in mature human spermatozoa. METHODS: Plasmid containing HIV gag DNA were constructed, and then transfected into the mature human spermatozoa. After 24 h culture, HIV gag mRNA was detected by RT_PCR. RESULTS: HIV gag mRNA could be detected in transfected mature human spermatozoa. CONCLUSION: HIV gag gene could be expressed at mRNA level in mature human spermatozoa.

Key words: human immunodeficiency virus_1, mature human spermatozoa, mRNA