›› 2011, Vol. 23 ›› Issue (3): 194-198.doi: 10.3969/j.issn.1004-616x.2011.03.008

• 论著 • 上一篇    下一篇

苯并(a)芘和滴滴涕联合暴露对小鼠肝脏细胞凋亡的影响

赵 苒,郭向宇,王 娟,苏艳华,郑 芳,范 春*   

  1. 厦门大学医学院预防医学系，福建 厦门 361005
  • 收稿日期:2010-12-16 修回日期:2011-01-26 出版日期:2011-05-30 发布日期:2011-05-30
  • 通讯作者: 范 春

Effects of benzo(a)pyrene and chlorophenothane exposure on apoptosis of liver cells in mice

ZHAO Ran，GUO Xiang-yu，WANG Juan，SU Yan-hua，ZHENG Fang，FAN Chun *   

  1. Department of Preventive Medicine, Medical College, Xiamen University, Xiamen 361005, Fujian, China
  • Received:2010-12-16 Revised:2011-01-26 Online:2011-05-30 Published:2011-05-30
  • Contact: FAN Chun

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摘要： 探讨不同剂量苯并(a)芘[benzo(a)pyrene， B(a)P]、滴滴涕(chlorophenothane， DDT)单独及联合暴露对小鼠肝脏细胞的毒性效应。 方法： 成年雌性昆明种小鼠66只，随机分为11组：分别为0.5、5、50 mg/(kg·d) B(a)P染毒组，0.025、0.25、2.5 mg/(kg·d) DDT染毒组，0.5 mg/(kg·d) B(a)P + 0.025 mg/(kg·d) DDT联合染毒组，5 mg/(kg·d) B(a)P + 0.25 mg/(kg·d) DDT联合染毒组，50 mg/(kg·d) B(a)P + 2.5 mg/(kg·d) DDT联合染毒组，空白对照组(正常饲养)和溶剂对照组(植物油处理)。染毒组用含B(a)P、DDT的食用油进行腹腔注射，每天1次，连续21 d，于末次给药24 h后处死小鼠。取肝脏制作冰冻切片，利用原位缺口末端标记(terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling， TUNEL)法检测肝细胞凋亡情况。 结果： 5和50 mg/(kg·d) B(a)P染毒组小鼠肝脏细胞凋亡率显著高于对照组 (P<0.05)，且50 mg/(kg·d)剂量组显著高于0.5和5 mg/(kg·d)剂量组(P<0.05)；各DDT染毒组与对照组比较，差异无统计学意义(P>0.05)；各浓度联合染毒组小鼠肝脏细胞凋亡率均高于对照组(P<0.01)，各联合染毒组细胞凋亡率间的差异无统计学意义(P>0.05)，联合染毒组细胞凋亡率和相应的B(a)P染毒组比较，差异无统计学意义(P>0.05)。 结论： B(a)P的单独暴露以及与DDT的联合暴露均可导致小鼠肝脏细胞凋亡的发生，并可能引发其他毒性效应。

关键词: 苯并(a)芘, 滴滴涕, 联合暴露, 肝细胞凋亡

Abstract: To study the effects of benzo(a)pyrene [B(a)P] and chlorophenothane(DDT) exposure alone or in combination on apoptosis of liver cells in mice. METHODS： 66 healthy female Kunming mice were randomly divided into 11 groups： 0.5， 5， 50 mg/(kg·d) B(a)P groups; 0.025， 0.25， 2.5 mg/(kg·d) DDT groups;0.5 mg/(kg·d) B(a)P + 0.025 mg/(kg·d) DDT group; 5 mg/(kg·d) B(a)P + 0.25 mg/(kg·d) DDT group; 50 mg/(kg·d) B(a)P + 2.5 mg/(kg·d) DDT group; blank control group (normal feed) and solvent control group(equal volume of oil). Exposure groups were injected with oil containing B(a)P and DDT once a day. After 21-day intraperitoneal injection， livers were taken for frozen sections. Apoptosis of liver cells was examined by TUNEL method. RESULTS：The apoptotic rates of 5 and 50 mg/(kg·d) B(a)P group were significantly higher than that of the control groups (P<0.01 or P<0.05)， besides the apoptotic rate of the 50 mg/(kg·d) B(a)P group was higher than that of the 0.5 and 5 mg/(kg·d) B(a)P groups (P<0.05). The apoptotic rates of the DDT groups had no significant difference with the control groups (P>0.05). In the combined exposure groups， apoptosis was more serious than that of the control groups (P<0.01)， while the comparison between the combined exposure groups showed no statistical difference(P > 0.05)， same with the combined groups and the corresponding B(a)P groups(P > 0.05). CONCLUSION：In these experimental conditions， 5 and 50 mg/(kg·d) B(a)P groups could cause liver cell apoptosis， and the higher the dose of B(a)P， the higher the apoptotic rate. No apoptosis could be demonstrated in 0.025， 0.25， 2.5 mg/(kg·d) DDT groups. All of the combined exposure groups could cause liver apoptosis， but there was no clear dose-response relationship observed. Above all， either B(a)P exposure alone or combined with DDT could cause liver cell apoptosis in mice. Whether it could cause other toxicological effects would be discussed in the future.

Key words: benzo(a)pyrene, chlorophenothane, combined exposure, apoptosis of liver cells

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