›› 2011, Vol. 23 ›› Issue (4): 260-262.doi: 10.3969/j.issn.1004-616x.2011.04.004

• 论著 • 上一篇    下一篇

环六亚甲基双乙酰胺诱导EC9706细胞分化中细胞增殖与形态结构的变化

高 升, 杨海波, 王一雯, 刘亚军, 余淮斌, 孙 婕, 谢朝晖, 陈兰英*   

  1. 河南城建学院生物工程系，河南 平顶山 467036
  • 收稿日期:2011-01-24 修回日期:2011-04-07 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 陈兰英

Proliferative and morphological changes of EC9706 cells induced by hexamethylene bisacetamide

GAO Sheng，YANG Hai-bo，WANG Yi-wen，LIU Ya-jun，YU Huai-bin，SUN Jie，XIE Zhao-hui，CHEN Lan-ying*   

  1. Department of Bioengineering, Henan University of Urban Construction, Pingdingshan 467036, Henan, China
  • Received:2011-01-24 Revised:2011-04-07 Online:2011-07-30 Published:2011-07-30
  • Contact: CHEN Lan-ying

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摘要： 目的： 观察环六亚甲基双乙酰胺 (hexamethylene bisacetamide，HMBA）对人食管癌 EC9706细胞的增殖以及形态结构的影响。 方法：采用不同浓度的HMBA (3、5、8、10 mmol/L）处理人食管癌EC9706细胞，实验同时设立对照组(未加HMBA)。测定各组细胞的生长曲线、分裂指数，并在光镜下观察HMBA诱导前后细胞增殖以及形态结构的变化。 结果：与对照组比较，经3、5、8、10 mmol/L的 HMBA处理后，EC9706细胞的生长和增殖受到抑制，其抑制率依次分别为52.875％、80.579％、83.617％、90.272％，差异均具有统计学意久(P＜0.05或P＜0.01)，且呈浓度-效应关系。光镜下观察HMBA处理组的EC9706细胞较对照组细胞的体积增大，且趋于扁平铺展状态，细胞大小较为一致，排列较为规则。 结论：经HMBA诱导处理后的人食管癌EC9706细胞出现了与同组织来源的正常上皮细胞相似的形态与结构特征，表现出肿瘤细胞恶性表型逆转的特征。

关键词: 细胞增殖, 细胞形态结构, 环六亚甲基双乙酰胺, 食管癌, EC9706细胞

Abstract: OBJECTIVE: To observe proliferative and morphological changes in human esophageal cancer cell differentiation induced by Hexamethylene Bisacetamide (HBMA). METHODS：EC9706 cells were treated by different concentration of HMBA(0，3，5，8，10 mmol/L) and the changes in proliferation and morphology before and after treatment were examined under light microscope ，while the effects were detected by growth curve，division index and HE staining. RESULTS：After treatment with HMBA the proliferation and growth of EC9706 cells were inhibited.，and the inhibitory rate rose with the increase of HBMA concentration. EC9706 cells tended to spread out flat，cell size was more consistent and regular under light microscope. CONCLUSION：Human esophageal cancer cells treated with HMBA appeared similar to normal epithelial cell morphology and structural features，displaying malignant phenotype reversal of tumor cells.

Key words: cell proliferation, cell morphological and structure, hexamethylene bisacetamide, esophageal cancer EC9706

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