›› 2011, Vol. 23 ›› Issue (4): 291-293.doi: 10.3969/j.issn.1004-616x.2011.04.011

• 论著 • 上一篇    下一篇

宫颈癌放疗患者外周血淋巴细胞DNA 损伤的研究

武丽蕊, 李红霞, 王兰朋, 王德华, 孙 莎   

  1. 1.中国石油天然气集团公司中心医院妇产科,河北 廊坊 065000;2. 廊坊市人民医院胸外科,河北 廊坊 065000;3. 天津医科大学总医院妇产科,天津 300070
  • 收稿日期:2010-12-29 修回日期:2011-04-20 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 武丽蕊

Evaluation of DNA damage induced by radiotherapy in cervical cancer patients

WU Li-rui,LI Hong-xia,WANG Lan-peng,WANG De-hua,SUN Sha   

  1. 1. Department of Gynecology and Obstetrics, China National Petroleum Company Central Hospital, Langfang 065000,Hebei;
  • Received:2010-12-29 Revised:2011-04-20 Online:2011-07-30 Published:2011-07-30
  • Contact: WU Li-rui

摘要: 目的: 评价宫颈癌放疗患者外周血淋巴细胞的DNA 损伤。方法:取15例宫颈鳞癌放疗患者放疗前及放疗累积剂量为10、20、30、40、50、60 Gy时的外周静脉血,以彗星实验检测淋巴细胞的DNA损伤。结果:各累积剂量组淋巴细胞DNA拖尾率比照射前显著升高 (P<0.01),并且在0~60 Gy之间,拖尾率与累积照射剂量之间成线性关系,回归方程为y=12.133+0.230x,相关系数r =0.964 (P<0.05)。各累积剂量组尾长比照射前均增加 (P<0.01),在照射剂量累积30 Gy时,尾长达最大。之后随照射累积剂量的进一步增加,尾长稍有下降,但是与照射前相比仍持续在较高的水平 (P<0.01),尾长与累积剂量间不成线性关系。结论:宫颈癌患者放疗后可造成外周血淋巴细胞DNA损伤。

关键词: 宫颈癌, 放疗, 彗星实验, 放疗损伤, 剂量-效应关系

Abstract: OBJECTIVE: To assess the DNA damage by comet assay in cervical cancer patients undergoing radiotherapy. METHODS:Blood samples were collected from 15 cervical cancer patients exposed to radiation at the accumulated dose of 0,10,20,30,40,50,60 Gy. DNA damage was measured by comet assay. RESULTS: Rate of lymphocyte DNA tailing in radiation groups increased significantly compared with to pre-radiation (P<0.01),showing a linear relationship from 0 to 60 Gy. The regression equation was y =12.133+0.230x,r =0.964 (P<0.05). The tail length showed significant increase in radiation groups compared with pre-radiation (P<0.01). At the accumulated dose of 30 Gy,the tail length reached the peak value. The tail length decreased a little,but remained at a higher level still when dose was higher than 30 Gy, compared to pre-radiation (0 Gy)(P<0.01). There was no dose-effect relationship between tail length and accumulated dose. CONCLUSION:Radiotherapy may induce DNA damage in cervical cancer patients. Evaluation of the radiation- damage by comet assay has a high sensitivity.

Key words: cervical cancer, radiotherapy, comet assay, radiation-induced damage, dose-effect relationship