用早熟凝集染色体环法研究中子诱发染色体畸变的剂量效应关系

doi:10.3969/j.issn.1004-616x.2011.06.011

›› 2011, Vol. 23 ›› Issue (6): 449-451.doi: 10.3969/j.issn.1004-616x.2011.06.011

用早熟凝集染色体环法研究中子诱发染色体畸变的剂量效应关系

王春燕,佟鹏,苟巧,郝述霞,齐雪松,吕慧敏,张伟

中国疾病预防控制中心辐射防护与核安全医学所，北京 100088

收稿日期:2011-09-19 修回日期:2011-10-12 出版日期:2011-11-30 发布日期:2011-11-30
通讯作者: 张伟

Dose-response study of neutron-induced human chromosome aberrations using prematurely condensed chromosomes ring method

WANG Chun-yan，TONG Peng，GOU Qiao，HAO Shu-xia，QI Xue-song，LU Hui-min，ZHANG Wei

National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China

Received:2011-09-19 Revised:2011-10-12 Online:2011-11-30 Published:2011-11-30
Contact: ZHANG Wei

摘要/Abstract

摘要： 目的：建立人外周血淋巴细胞早熟凝集染色体环 (prematurely condensed chromosomes ring，PCC-R)与中子辐射剂量之间的效应关系曲线。方法：选择健康成年男性2人，取静脉血 (肝素抗凝)，用中子反应堆分别照射0、1.0、 2.5、 5.0、7.5和10.0 Gy (剂量率为0.2 Gy/min)，照射后的血样在37 ℃恒温培养箱内静置3 h以进行细胞修复，然后将血样分别接种到RPMI 1640培养基中培养48 h。终止培养前1 h加入500 nmol/L蛋白磷酸酶抑制剂冈田酸，观察辐射诱发的人外周血淋巴细胞早熟凝集染色体环与照射剂量之间的效应关系。结果：500 nmol/L的冈田酸诱导的人外周血淋巴细胞早熟凝集染色体中早熟凝集染色体环随着照射剂量的增加而增加，并呈直线相关 (y=0.097 6x+0.000 3，R2=0.980 5)。结论：早熟凝集染色体环随着照射剂量的增加而增加，它可作为生物剂量指示计用于受到中子照射后的剂量估算，特别是在受到大剂量照射的情况。

关键词: 中子, 生物剂量, 外周血淋巴细胞, 早熟凝集染色体环

Abstract: OBJECTIVE: To establish dose-response relationship between prematurely condensed chromosome (PCC) rings of human peripheral lymphocytes and neutron radiation. METHODS：Heparinized peripheral blood was taken from two healthy adult females and irradiated by 0，1.0，2.5，5.0，7.5 and 10.0 Gy of neutron (dose rate 0.2 Gy/min). Blood was kept in incubator (37 ℃) for 3 h for cell repair. Then blood was cultured with RPMI 1640 for 48 hrs. 1 hr before the end of culture，Okadaic acid，at final concentration of 500 nmol/L， was added into culture medium to induce PCC rings. Yield of PCC rings at each dose point after staining with Giemsa was counted and the relationship between PCC rings of human peripheral lymphocytes induced by neutron radiation was examined. RESULTS：In 500 nmol/L Okadaic-acid-induced prematurely condensed chromosomes of human peripheral lymphocytes， the yield of PCC rings increased with the dose of radiation and showing a linear correlation (y=0.097 6x+0.000 3， R2=0.980 5). CONCLUSION： A good dose-response relationship was found between the yield of PCC rings and radiation dose from 0 to 10 Gy. The yield of PCC rings could be used as a biodosimeter in neutron irradiation，especially for high dose.

Key words: neutron, biodosimeter, peripheral blood lymphocytes, prematurely condensed chromosomes

王春燕,佟鹏,苟巧,郝述霞,齐雪松,吕慧敏,张伟. 用早熟凝集染色体环法研究中子诱发染色体畸变的剂量效应关系[J]. , 2011, 23(6): 449-451.

WANG Chun-yan，TONG Peng，GOU Qiao，HAO Shu-xia，QI Xue-song，LU Hui-min，ZHANG Wei. Dose-response study of neutron-induced human chromosome aberrations using prematurely condensed chromosomes ring method[J]. , 2011, 23(6): 449-451.

[1]	张忠新, 张睿凤, 张慧芳, 段志凯. 常用辐射生物剂量计的研究现状[J]. 癌变·畸变·突变, 2016, 28(4): 325-328.
[2]	姚波,李玉芳,白娟,周振山,王军良,满秋红,邱丽娟,刘广贤,郭梅,艾辉胜. 早熟凝集染色体环法在山西太原事故受照射者生物剂量估算中的应用[J]. 癌变·畸变·突变, 2013, 25(2): 91-95.
[3]	刘瑞祥1/任嘉红1,2/常惠丽3/霍转转1/刘彤1/闫科峰1. 浊漳河水体中有机污染物的遗传毒性研究[J]. 癌变·畸变·突变, 2012, 24(6): 465-469.
[4]	汪传高，王治东，陈茂胜,杜杰,张学清,王仲文,陈英. 提前获取早熟凝集染色体环快速估算受照剂量的可行性研究[J]. , 2011, 23(6): 446-448.
[5]	王雷,王治东,胡海亮,张学清,陈茂胜,薛绍礼,陈英. 外周血网织红细胞和T淋巴细胞亚群用于辐射损伤快速剂量估算的可行性研究[J]. , 2011, 23(6): 452-455.
[6]	段　链/张宏伟. 维生素C对水中有机物致小鼠外周血淋巴细胞DNA损伤的抑制作用[J]. , 2007, 19(3): 246-250.
[7]	艾军;梁索源;单保恩;何兰欣;任金荣. 食管癌患者外周血淋巴细胞替代肿瘤细胞体外药敏试验相关性研究[J]. , 2006, 18(1): 58-060.
[8]	孙立平，李德志，刘志谋，杨录军，刘晋，曹　佳. 人外周血网织红细胞微核作为辐射生物剂量仪的初步研究[J]. , 2004, 16(3): 163-166.
[9]	邢万金1, 王宇一2. 7 种农药对植物及人外周淋巴细胞SCE影响的比较观察[J]. , 2001, 13(3): 172-173.
[10]	吕玉民1 　韩　林1 　赵　燕2 　马良庆1. 印刷作业女工外周血淋巴细胞微核观察[J]. , 1999, 11(2): 101-103.

用早熟凝集染色体环法研究中子诱发染色体畸变的剂量效应关系

Dose-response study of neutron-induced human chromosome aberrations using prematurely condensed chromosomes ring method

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价