血浆中apoAⅠ和mTOR蛋白在维吾尔族妇女宫颈病变进程中的动态变化及意义

doi:10.3969/j.issn.1004-616x.2014.03.006

癌变·畸变·突变

血浆中apoAⅠ和mTOR蛋白在维吾尔族妇女宫颈病变进程中的动态变化及意义

郭霞¹，金华²，玛依拉·卡米力江¹，刘开江²，阿仙姑·哈斯木³，哈丽丹·热依木¹，武贵臻¹，Abulizi·ABUDULA ^1，*

新疆医科大学新疆地方病分子生物学重点实验室，新疆乌鲁木齐 830011

收稿日期:2013-06-06 修回日期:2014-03-21 出版日期:2014-05-30 发布日期:2014-05-30
通讯作者: Abulizi·ABUDULA，E-mail：abulizi_de@126.com
作者简介:郭霞(1980－ )，女，陕西省人，博士，助理研究员，研究方向：宫颈癌的发生机制与预警。E-mail：myshow0504@163.com
基金资助:
国家自然科学基金项目(81260380)

Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women

GUO Xia¹，JIN Hua²，Mayila·KAMILIJIANG¹，LIU Kai-jiang²，Axiangu·HASIMU ³，Halidan·REYIMU¹，WU Gui-zhen¹，Abulizi·ABUDULA^1，*

Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi 830011

Received:2013-06-06 Revised:2014-03-21 Online:2014-05-30 Published:2014-05-30

摘要/Abstract

摘要：

目的：探讨血浆中载脂蛋白AⅠ(apoAⅠ)含量和哺乳动物雷帕霉素靶蛋白(mTOR)活性在维吾尔族妇女宫颈病变进程中的动态变化及评价其对于宫颈癌预警的灵敏度、特异性和准确性。方法：收集新疆地区维吾尔族宫颈病变血浆样本共82例(宫颈炎22例，CINⅡ~Ⅲ20例，宫颈癌早期20例，宫颈癌晚期20例)，用酶联免疫法检测血浆中apoAⅠ蛋白含量和mTOR活性，分析其与宫颈病变进程的关系及相关性，评价其对于宫颈癌预警的灵敏度、特异性和准确性。结果：随着宫颈炎到CINⅡ~Ⅲ、宫颈癌早期和晚期发病过程，血浆apoAⅠ含量明显降低而mTOR活性上升，与正常对照宫颈炎组比较，其差异均有统计学意义(P＜0.05)；apoAⅠ的表达与mTOR活性呈低度负相关(r＜0.4，P＜0.05)；血浆mTOR活性的预警灵敏度、特异度和准确度较高，分别为94.0%、60.0%、80.0%，而apoAⅠ分别为70.1%、60.0%、29.0%。从不同指标联合检测的角度分析，apoAⅠ和mTOR联合检测的灵敏度、特异度和准确度分别为82.1%、60.0%、54.5%。结论：维吾尔族妇女宫颈癌发生发展与血浆中apoAⅠ含量和mTOR活性动态变化密切相关，可以成为宫颈癌早期诊断的预警或疗效评估的重要指标。

关键词: 维吾尔族妇女, 宫颈病变, 载脂蛋白AⅠ, 哺乳动物雷帕霉素靶蛋白, 血浆生物标志物

Abstract:

OBJECTIVE: To explore the relationships of the dynamic changes of plasma apoAⅠcontent and mTOR activity with cervical carcinogenesis development in Uyghur women. METHODS：We collected 82 plasma samples from Uyghur women with cervicitis (CV) (22 cases)，cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ (20 cases)，early stage (Ⅰ-Ⅱ) (20 cases) and advanced stage (ⅡB-Ⅳ) (20 cases) cervical squamous cell carcinoma， quantitatively determined the content of plasma proteins apoAⅠand the activity of mTOR by enzyme linked immunosorbent assay (ELISA) and statistical analysis，in order to analyze the correlation with the progression of cervical lesion and evaluate the accuracy，specificity and sensitivity of candidate plasma protein markers for early warning of cervical cancer. RESULTS：With the development of cervicitis to CINⅡ-Ⅲ，early stage or advanced stage cervical cancer，the changes of two plasma proteins altered gradually，with obvious decrease in the content of apoAⅠ，and increase in the activity of mTOR，with statistical differences for all (P<0.05). Correlation analysis of cervical cancer-specific protein indicators found that mTOR and apoAⅠshowed a low negative correlation (r＜0.4，P＜0.05). The sensitivity，specificity，and accuracy of detection，for early warning of cervical cancer of the mTOR protein were 94.0%， 60.0% and 80.0%，respectively；and for apoAⅠwere 70.1%， 60.0% and 29.0%，respectively. apoAⅠ/mTOR combined detection of sensitivity，specificity， and accuracy were 82.1%，60.0% and 54.5%，respectively. CONCLUSION：The dynamic changes of apoAⅠcontent and mTOR activity were important indicators of the procession of cervical lesions，and expected to assist with the early diagnosis of cervical cancer.

Key words: Uyghur women, cervical carcinogenesis, apoAⅠ, mTOR, plasma biomarker

郭霞，金华，玛依拉·卡米力江，刘开江，阿仙姑·哈斯木，哈丽丹·热依木，武贵臻，Abulizi·ABUDULA . 血浆中apoAⅠ和mTOR蛋白在维吾尔族妇女宫颈病变进程中的动态变化及意义[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2014.03.006.

GUO Xia，JIN Hua，Mayila·KAMILIJIANG，LIU Kai-jiang，Axiangu·HASIMU,Halidan·REYIMU，WU Gui-zhen，Abulizi·ABUDULA. Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.03.006.

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血浆中apoAⅠ和mTOR蛋白在维吾尔族妇女宫颈病变进程中的动态变化及意义

Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women

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