nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响

doi:10.3969/j.issn.1004-616x.2015.02.012

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (2): 134-136,141.doi: 10.3969/j.issn.1004-616x.2015.02.012

nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响

郑海霞¹, 崔永言², 申东兰³, 彭安³, 何艳玲³

1. 北京大学深圳医院体检科, 广东深圳 518036;
2. 北京大学深圳医院整形外科, 广东深圳 518036;
3. 北京大学深圳医院肿瘤内科, 广东深圳 518036

收稿日期:2014-06-04 修回日期:2014-12-03 出版日期:2015-03-31 发布日期:2015-03-31
通讯作者: 崔永言,E-mail:cyy8188@163.com E-mail:cyy8188@163.com
作者简介:郑海霞,Tel:13828736582,E-mail:zhenghx200878@163.com
基金资助:
国家自然科学基金资助项目(30070333)

Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981

ZHENG Haixia¹, CUI Yongyan², SHEN Donglan³, PENG An³, HE Yanling³

1. Department of Health Examination, Peking University Shenzhen Hospital, Shenzhen 518036;
2. Department of Plastic Surgery, Peking University Shenzhen Hospital, Shenzhen 518036;
3. Department of Oncology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China

Received:2014-06-04 Revised:2014-12-03 Online:2015-03-31 Published:2015-03-31
About author:10.3969/j.issn.1004-616x.2015.02.012

摘要/Abstract

摘要：

目的:探讨nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响。方法:利用细胞黏附实验、Boyden小室法研究转染前后肺癌L9981细胞黏附、侵袭能力的改变。分别利用逆转录-PCR(RT-PCR)和流式细胞术检测转染前后E-cadherin、integrin β1和integrin β3 mRNA和蛋白的表达。结果:转染后L9981细胞株黏附性以及侵袭性均降低。与转染空载体组相比,转染nm23-H1质粒后L9981细胞E-cadherin mRNA和蛋白表达均增强,integrin β1和integrin β3 mRNA和蛋白表达均减弱,差异均具有统计学意义(P均<0.05)。结论:nm23-H1基因具有逆转肺癌恶性转移表型的能力,其对肺癌L9981细胞株E-cadherin表达具有正调控作用,对integrin β1和integrin β3表达具有负调控作用。

关键词: nm23-H1, 肺癌细胞, 钙调素, 整合素, 肿瘤转移

Abstract:

OBJECTIVE:To study influences of nm23-H1 gene transfection on the expressions of adhesion molecules in lung cancer cell line L9981. METHODS:Cell adhesion experiment and Boyden room were used to evaluate the change of cell adhesion and invasion. Semi-RT-PCR and flow cytometry were used to detect the mRNA and protein expressions of E-cadherin, integrin β1 and integrin β3 betwteen transfected and non-transfected cell lines. RESULTS: nm23-H1 cell line in vitro adhesion and invasion were all down-regulated. mRNA of E-cadherin in the transfected cell line was stronger than that of non-transfected cell line, but mRNAs of integrin β1 and integrin β3 of transfected cell line were weaker than that of non-transfected cell line. Flow cytometry showed similar results as above. CONCLUSION: nm23-H1 could reverse lung cancer malignant phenotype. nm23-H1 could up-regulate E-cadherin expression, while down-regulate expressions of integrins β1 and β3, thus inhibit the metastasis of lung cancer cells.

Key words: nm23-H1, lung cancer cells, E-cadherin, integrins, neoplastic metastasis

中图分类号:

R730.2

郑海霞, 崔永言, 申东兰, 彭安, 何艳玲. nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响[J]. 癌变·畸变·突变, 2015, 27(2): 134-136,141.

ZHENG Haixia, CUI Yongyan, SHEN Donglan, PENG An, HE Yanling. Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 134-136,141.

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nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响

Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981

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