癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (2): 146-149,153.doi: 10.3969/j.issn.1004-616x.2015.02.015

• 检测研究 • 上一篇    下一篇

海滩牵牛正丁醇提取物化学成分的抗炎活性研究

郑付春1, 陈一村2, 秦永洁2, 蔡聪艺2,3   

  1. 1. 汕头大学医学院第一附属医院药剂科, 广东汕头 515041;
    2. 汕头大学医学院药理学教研室, 广东 汕头 515041;
    3. 汕头大学医学院第二附属医院药剂科, 广东 汕头 515041
  • 收稿日期:2014-12-30 修回日期:2015-02-02 出版日期:2015-03-31 发布日期:2015-03-31
  • 通讯作者: 蔡聪艺,E-mail:skyccy@sina.com E-mail:skyccy@sina.com
  • 作者简介:郑付春,E-mail:zhengfc@stu.edu.cn
  • 基金资助:

    广东省自然科学基金-团队项目(9351503102000001);中央财政支持地方高校发展专项资金

Anti-inflammatory activity of chemical components from n-butanol extract of Ipomoea stolonifera

ZHENG Fuchun1, CHEN Yicun2, QIN Yongjie2, CAI Congyi2,3   

  1. 1. Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College, Shantou 515041;
    2. Department of Pharmacology, Medical College, Shantou University, Shantou 515041;
    3. Department of Pharmacy, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2014-12-30 Revised:2015-02-02 Online:2015-03-31 Published:2015-03-31
  • About author:10.3969/j.issn.1004-616x.2015.02.015

摘要:

目的:对潮汕地区草药海滩牵牛(IS)正丁醇提取物(BE-IS)化学成分进行分离纯化,并研究这些成分在体内的抗炎活性。方法:利用多种色谱法对BE-IS中的化学成分进行分离,通过波谱解析方法确定它们的结构;在巴豆油致小鼠耳肿胀实验、角叉菜胶致大鼠足肿胀实验等炎症模型上评价BE-IS化学成分的抗炎活性。结果:BE-IS分离得到5个单体,分别为东莨菪亭、伞形花内酯、秦皮乙素、橙皮素和姜黄素。与对照组比较,BE-IS单体均能不同程度地抑制巴豆油所致小鼠耳肿胀、角叉菜胶所致大鼠足肿胀,差异均有统计学意义(P<0.01或P<0.05)。结论:首次从海滩牵牛分离得到的5个单体化合物具有较好的体内抗炎活性,揭示了海滩牵牛抗炎活性的物质基础。

关键词: 海滩牵牛, 化学成分, 抗炎活性, 香豆素, 姜黄素

Abstract:

OBJECTIVE:To separate and refine the chemical components from n-butanol extract of Ipomoea stolonifera (BE-IS) in Chaoshan area, and investigate their anti-inflammatory activities in vivo. METHODS:The chemical components from BE-IS were separated by different kinds of chromatography and identified by spectrum analysis methods. Then anti-inflammatory activity was evaluated in croton oil-induced ear edema model and carrageenan-induced paw edema model. RESULTS:Scopoletin, umbelliferone, esculetin, hesperetin and curcumin were extracted from BE-IS. Compared to the control group, compounds from BE-IS could inhibit the edema induced by croton oil or carrageenan to different degree, and the difference was statistically significant (P<0.01 or P<0.05). CONCLUSION: The chemical components from BE-IS have anti-inflammatoty activities, which explained why Ipomoea stolonifera possess strong anti-inflammatoty activity.

Key words: Ipomoea stolonifera, chemical components, anti-inflammatory activity, coumarin, curcumin

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