TMEM196基因在肺癌中的甲基化修饰与表达调控

doi:10.3969/j.issn.1004-616x.2015.05.005

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (5): 353-356.doi: 10.3969/j.issn.1004-616x.2015.05.005

TMEM196基因在肺癌中的甲基化修饰与表达调控

赵欢^1,2, 刘文斌¹, 陈洪强¹, 韩飞¹, 曹佳¹, 刘晋祎¹

1. 第三军医大学军事预防医学院毒理研究所, 重庆 400038;
2. 第三军医大学第一附属医院, 重庆 400038

收稿日期:2015-08-15 修回日期:2015-09-12 出版日期:2015-09-30 发布日期:2015-09-30
通讯作者: 刘晋祎,E-mail:jinyiliutmmu@163.com E-mail:jinyiliutmmu@163.com
作者简介:赵欢,E-mail:merryjojo@163.com。
基金资助:
国家自然科学基金(81202238)

TMEM196 gene methylation and expression in lung cancer

ZHAO Huan^1,2, LIU Wenbin¹, CHEN Hongqiang¹, HAN Fei¹, CAO Jia¹, LIU Jinyi¹

1. Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing 400038;
2. The First Affiliated Hospital of Third Military Medical University, Chongqing 400038, China

Received:2015-08-15 Revised:2015-09-12 Online:2015-09-30 Published:2015-09-30

摘要/Abstract

摘要：

目的:分析TMEM196基因在肺癌组织中的甲基化情况以及甲基化发生对TMEM196基因表达的影响。方法:采用MSP方法检测肺癌组织、癌旁正常对照组织及肺癌细胞株TMEM196基因甲基化率;用去甲基化药物5-氮-2'-脱氧胞苷(5-aza-dC)处理肺癌细胞后,采用RT-PCR法检测TMEM196 mRNA表达水平。结果:肺癌组织中TMEM196基因甲基化发生率为57%(28/49),而正常对照组织中该基因甲基化发生率为0(0/20)。TMEM196基因甲基化与肿瘤的分化程度(P=0.012)和临床分期显著相关(P=0.007),而与患者的年龄、性别、吸烟以及肿瘤的病理分类无显著相关(P>0.05)。TMEM196基因在肺癌细胞株H1975和H1650中高甲基化且表达缺失,去甲基化药物(5-aza-dC)处理细胞后TMEM196 mRNA表达明显升高,说明TMEM196基因表达可能受甲基化调控。结论:TMEM196基因甲基化与肺癌的发生发展密切相关,推测该基因通过DNA甲基化失活参与了肿瘤的发生。

关键词: TMEM196基因, 肺癌, DNA甲基化, 表达

Abstract:

OBJECTIVE: To analyze TMEM196 gene methylation and expression in lung cancer. METHODS: Methylation-specific PCR was used to detect the DNA methylation status of TMEM196 gene in normal and tumor tissues and cell lines. RT-PCR was used to analyze the expression of TMEM196 gene in tumor cells and also in 5-aza-dC-treated tumor cells. RESULTS:The hypermethylation of TMEM196 was detected in lung cancer tissues (57%, 28/49), but not in normal tissues (0, 0/20). Methylation of TMEM196 gene was significantly correlated with tumor differentiation (P=0.012) and the clinical stages of lung cancer patients (P=0.007), but not correlated with the patient's age, sex, smoking, and pathological classification (P>0.05). TMEM196 showed hypermethylation and its expression decreased in lung cancer cell lines H1975 and H1650. After treatment with demethylation drug, expression of TMEM196 gene increased significantly in these cell lines. CONCLUSION:The expression of TMEM196 gene was inhibited through hypermethylation in lung cancer. This process may play an important role in lung cancer.

Key words: TMEM196 gene, lung cancer, DNA methylation, expression

中图分类号:

R734.2

赵欢, 刘文斌, 陈洪强, 韩飞, 曹佳, 刘晋祎. TMEM196基因在肺癌中的甲基化修饰与表达调控[J]. 癌变·畸变·突变, 2015, 27(5): 353-356.

ZHAO Huan, LIU Wenbin, CHEN Hongqiang, HAN Fei, CAO Jia, LIU Jinyi. TMEM196 gene methylation and expression in lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 353-356.

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TMEM196基因在肺癌中的甲基化修饰与表达调控

TMEM196 gene methylation and expression in lung cancer

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