玉麦须醇提物对L929细胞氧化应激损伤的保护作用

doi:10.3969/j.issn.1004-616x.2015.06.004

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 426-431.doi: 10.3969/j.issn.1004-616x.2015.06.004

玉麦须醇提物对L929细胞氧化应激损伤的保护作用

方从文, 柏桦, 张晓迪, 刘瑞, 海春旭

第四军医大学军事预防医学院军事毒理学教研室, 陕西西安 710032

收稿日期:2015-03-20 修回日期:2015-09-14 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 柏桦,E-mail:hua_bai@126.com E-mail:hua_bai@126.com
作者简介:方从文,E-mail:447354609@qq.com。

Protective effects of maize silks ethanol extract on oxidative injury in L929 cells

FANG Congwen, BAI Hua, ZHANG Xiaodi, LIU Rui, HAI Chunxu

Department of Military Toxicology, School of Military Prevention Medicine, the Fourth Military Medicine University, Xi'an 710032, Shannxi, China

Received:2015-03-20 Revised:2015-09-14 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:观察不同剂量玉麦须醇提物(MSE)对照射所致L929细胞氧化应激损伤的保护作用及其可能的机制。方法:⁶⁰Co照射L929细胞后,即分别给予0、10、50、250μg/mL MSE处理,阴性对照组屏蔽照射,检测细胞氧化损伤、抗氧化系统、炎症因子等相关氧化应激指标的变化。结果:⁶⁰Co照射能引起显著的细胞氧化应激损伤,MSE处理后能降低硫代巴比妥酸反应产物(TBARS)含量,提高过氧化氢酶和过氧化物歧化酶的活性、增加GSH/GSSG比值,且存在一定剂量效应关系。同时,MSE能上调Nrf2和γ谷氨酰半胱氨酸合成酶的表达,抑制转化生长因子β(TGF-β)的基因和蛋白表达,以中剂量组(50μg/mL)作用最为显著。结论:一定剂量的MSE可改善由照射引发的细胞氧化应激损伤,Nrf2和TGF-β可能为玉麦须有效的作用靶点。

关键词: 玉麦须, ⁶⁰Co照射, 氧化应激, 抗氧化

Abstract: OBJECTIVE: To investigate the protective effects of maize silks ethanol extract(MSE) against radiation-induced oxidative damage in cells. METHODS: γ-radiation was employed to induce oxidative stress in L929 cells. After radiation,cells were treated immediately with different doses of MSE(10,50,250μg/mL). The variation of oxidative damage indicator,antioxidant system and inflammatory factors were examined. RESULTS: Radiation caused obvious oxidative injury in cells. MSE treatment significantly abolished elevation of thiobarbituric acid reactive substances(TBARS) levels,increased hepatic GSH/GSSG ratio and activities of catalase and superoxide dismutase. Moreover,MSE up-regulated the gene expressions of NF-E2-related factor 2 and gamma glutamyl cysteine synthetase. The gene and protein expressions of transforming growth factor beta(TGF-β)were also inhibited. Interestingly,50μg/mL MSE treatment performed best in our most results. CONCLUSION: These findings proved the protective role of MSE against radiationinduced oxidative injury. Nrf2 and TGF-β could be the potential targets of maize silks.

Key words: maize silks, ⁶⁰Co radiation, oxidative stress, antioxidant

中图分类号:

Q345+.7

方从文, 柏桦, 张晓迪, 刘瑞, 海春旭. 玉麦须醇提物对L929细胞氧化应激损伤的保护作用[J]. 癌变·畸变·突变, 2015, 27(6): 426-431.

FANG Congwen, BAI Hua, ZHANG Xiaodi, LIU Rui, HAI Chunxu. Protective effects of maize silks ethanol extract on oxidative injury in L929 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 426-431.

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玉麦须醇提物对L929细胞氧化应激损伤的保护作用

Protective effects of maize silks ethanol extract on oxidative injury in L929 cells

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