VEGF介导微囊藻毒素-LR促进结直肠癌细胞DLD-1侵袭迁移的研究

doi:10.3969/j.issn.1004-616x.2015.06.007

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 441-445.doi: 10.3969/j.issn.1004-616x.2015.06.007

VEGF介导微囊藻毒素-LR促进结直肠癌细胞DLD-1侵袭迁移的研究

任岩, 秦伟, 朱强强, 周丽华, 王婷

南京医科大学基础医学院细胞生物学系, 江苏南京 210029

收稿日期:2015-06-15 修回日期:2015-10-30 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 王婷,E-mail:wangting@njmu.edu.cn E-mail:wangting@njmu.edu.cn
作者简介:任岩,E-mail:renyanren@yeah.net;秦伟,E-mail:qinweiab@foxmail.com。
基金资助:
2014江苏省大学生创新训练计划项目(KY101J201411)

VEGF expression in microcystin-LR promotion of invasion and migration in DLD-1 cells

REN Yan, QIN Wei, ZHU Qiangqiang, ZHOU Lihua, WANG Ting

Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2015-06-15 Revised:2015-10-30 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:研究微囊藻毒素-LR是否调节结直肠癌细胞VEGF的表达,进而影响肿瘤细胞的侵袭与迁移。方法:结直肠癌细胞DLD-1经过不用浓度微囊藻毒素-LR处理后,Transwell实验分析其侵袭、迁移能力的改变。实时荧光定量PCR(qPCR)、Westernblot以及ELISA实验检测DLD-1细胞中VEGF mRNA和蛋白表达水平的变化。结果:Transwell实验结果表明,与对照组比较,微囊藻毒素-LR处理组细胞侵袭、迁移能力明显提高,当微囊藻毒素-LR的浓度达到12.5 nmol/L时,侵袭、迁移能力分别提高了2.1和2.0倍;qPCR、Western blot结果显示微囊藻毒素-LR上调了结直肠癌细胞DLD-1中VEGF mRNA以及蛋白的表达水平,当微囊藻毒素-LR的浓度达到12.5和25 nmol/L时,DLD-1细胞中VEGF mRNA和蛋白水平分别达到最大值,上升了1.7和2.8倍;同时ELISA实验也检测到在细胞培养液中DLD-1细胞分泌的VEGF提高了1.5倍。结论:微囊藻毒素-LR可以显著上调DLD-1细胞中VEGF的表达,进而介导肿瘤细胞发生侵袭与迁移。

关键词: 微囊藻毒素-LR, 结直肠癌, 侵袭迁移, 血管内皮生长因子

Abstract: OBJECTIVE: This study investigated whether microcystin-LR regulated the expression of VEGF and promoted invasion and migration in colorectal DLD-1 cells. METHODS: Transwell experiments were used to analyze the invasion and migration in colorectal cancer DLD-1 cells treated with different concentrations of microcystin-LR. Expression of VEGF in treated cells was evaluated by real-time quantitative PCR(qPCR),Western blot and ELISA. RESULTS: That the abilities of invasion and migration improved significantly in microcystin-LR-treated groups,compared wiose in the control group. When the concentration of microcystin-LR reached 12.5 nmol/L,invasion and migration were increased to 2.1 and 2.0 folds,respectively. The qPCR and Western blot results suggested that microcystin-LR upregulated the expression level of VEGF mRNA and protein in DLD-1 cells. When microcystin-LR concentration reached 12.5 and 25 nmol/L,the expression levels of the VEGF protein and mRNA in DLD-1 cells reached a maximum of 1.7 and 2.8 folds increases,respectively. Mean while,the significant 1.5-foldelevated VEGF production in the culture medium was also detected. CONCLUSION: Microcystin-LR upregulation of VEGF was involved in promoting invasion and migration in colorectal cancer cells DLD-1.

Key words: microcystin-LR, colorectal cancer, invasion and migration, vascular endothelial growth factor

中图分类号:

R73-37

任岩, 秦伟, 朱强强, 周丽华, 王婷. VEGF介导微囊藻毒素-LR促进结直肠癌细胞DLD-1侵袭迁移的研究[J]. 癌变·畸变·突变, 2015, 27(6): 441-445.

REN Yan, QIN Wei, ZHU Qiangqiang, ZHOU Lihua, WANG Ting. VEGF expression in microcystin-LR promotion of invasion and migration in DLD-1 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 441-445.

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VEGF介导微囊藻毒素-LR促进结直肠癌细胞DLD-1侵袭迁移的研究

VEGF expression in microcystin-LR promotion of invasion and migration in DLD-1 cells

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