纳米SiO2颗粒对斑马鱼行为变化的影响

doi:10.3969/j.issn.1004-616x.2015.06.013

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 467-471,476.doi: 10.3969/j.issn.1004-616x.2015.06.013

纳米SiO₂颗粒对斑马鱼行为变化的影响

李旭玲^1,2, 吴德生², 洪文旭³, 刘建军², 柯跃斌², 袁建辉², 黎双飞¹

1. 深圳大学生命与海洋科学学院, 广东深圳 518060;
2. 深圳市疾病预防控制中心, 广东深圳 518055;
3. 深圳市人口和计划生育科学研究所, 广东深圳 518040

收稿日期:2015-09-15 修回日期:2015-10-25 出版日期:2015-11-30 发布日期:2015-11-30
通讯作者: 黎双飞,Email:sfli@szu.edu.cn;袁建辉,E-mail:jianhui_yuan@126.com E-mail:sfli@szu.edu.cn;jianhui_yuan@126.com
作者简介:李旭玲,E-mail:541327143@qq.com。
基金资助:
深圳市科技研发资金资助项目(JCYJ20140410164217365,JCY20140410171018510);广东省人口计生委科研项目(20132016);深圳市卫生计生委科研项目(201506064,201506065);深圳市城管局科研项目(201508)

Effects of nano-SiO₂ particles on zebrafish behavior response

LI Xuling^1,2, WU Desheng², HONG Wenxu³, LIU Jianjun², KE Yuebin², YUAN Jianhui², LI Shuangfei¹

1. College of Life and Marine Science, Shenzhen University, Shenzhen 518060;
2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055;
3. Shenzhen Research Institute of Population and Family Planning, Shenzhen 518040, Guangdong, China

Received:2015-09-15 Revised:2015-10-25 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:研究纳米SiO₂颗粒(SiO₂-NPs)暴露下斑马鱼行为学的变化,并对其变化规律进行分析,以期对突发的SiO₂-NPs污染实现早期的预警。方法:利用生物在线监测仪,以正常水质中斑马鱼的行为变化为基础,比较300、600、1200 mg/L SiO₂-NPs暴露下斑马鱼的个体行为(包括游动速度、游动高度、转弯次数和加速度)和群体行为(包括平均距离和分散度)的变化规律。结果:与正常水质比较,600和1200 mg/L SiO₂-NPs暴露下,斑马鱼的游动行为发生改变,游动速度在6 h时的降幅达到30%~50%,与暴露前相比差异显著(P均<0.01);游动高度的改变稍滞后于游动速度,在19 h时迅速下降,24 h时降幅达到90%以上(P<0.01);斑马鱼原有群体行为也发生改变,平均距离和分散度在24 h时均显著下降(P均<0.01)。结论:SiO₂-NPs暴露下,斑马鱼的行为短时间内变化明显,且随着浓度的增加,变化越明显,基本符合环境压力模型。

关键词: 纳米SiO₂颗粒, 斑马鱼, 行为学, 生物在线检测

Abstract: OBJECTIVE: To establish the early warning of the contamination of nano-SiO₂ particle,swimming behavior changes of zebrafish under nano-SiO₂ particle exposure were studied. METHODS: Based on behavior response of zebrafish,effects of nano-SiO₂particles,including individual endpoints(swimming speed,swimming depth,turning frequency and acceleration) and group endpoints(distance and dispersion),were investigated by using Online Bio monitoring. RESULTS: Swimming behavior changes of zebrafish were affected significantly in 600 and 1200 mg/L exposure dose groups. Swimming speed was decreased 30%-50% 6 h after exposure. Changes of swimming depth lagged behind changes of swimming speed,which were decreased by more than 90% 24 h after exposure. Group behavior of zebrafish was also changed,distance and dispersion were significantly decreased(all P<0.01). CONCLUSION: After exposure,swimming behavior of zebrafish(swimming speed,swimming depth,turning frequency,distance and dispersion) were affected significantly wie increase of nano-SiO₂ particles concentration,which is consistend wie Stepwise Stress Model.

Key words: nano-SiO₂ particles, zebrafish, behavior, biological monitoring

中图分类号:

X835

李旭玲, 吴德生, 洪文旭, 刘建军, 柯跃斌, 袁建辉, 黎双飞. 纳米SiO₂颗粒对斑马鱼行为变化的影响[J]. 癌变·畸变·突变, 2015, 27(6): 467-471,476.

LI Xuling, WU Desheng, HONG Wenxu, LIU Jianjun, KE Yuebin, YUAN Jianhui, LI Shuangfei. Effects of nano-SiO₂ particles on zebrafish behavior response[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 467-471,476.

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纳米SiO₂颗粒对斑马鱼行为变化的影响

Effects of nano-SiO₂ particles on zebrafish behavior response

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