癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 467-471,476.doi: 10.3969/j.issn.1004-616x.2015.06.013

• 检测研究 • 上一篇    下一篇

纳米SiO2颗粒对斑马鱼行为变化的影响

李旭玲1,2, 吴德生2, 洪文旭3, 刘建军2, 柯跃斌2, 袁建辉2, 黎双飞1   

  1. 1. 深圳大学生命与海洋科学学院, 广东 深圳 518060;
    2. 深圳市疾病预防控制中心, 广东 深圳 518055;
    3. 深圳市人口和计划生育科学研究所, 广东 深圳 518040
  • 收稿日期:2015-09-15 修回日期:2015-10-25 出版日期:2015-11-30 发布日期:2015-11-30
  • 通讯作者: 黎双飞,Email:sfli@szu.edu.cn;袁建辉,E-mail:jianhui_yuan@126.com E-mail:sfli@szu.edu.cn;jianhui_yuan@126.com
  • 作者简介:李旭玲,E-mail:541327143@qq.com。
  • 基金资助:
    深圳市科技研发资金资助项目(JCYJ20140410164217365,JCY20140410171018510);广东省人口计生委科研项目(20132016);深圳市卫生计生委科研项目(201506064,201506065);深圳市城管局科研项目(201508)

Effects of nano-SiO2 particles on zebrafish behavior response

LI Xuling1,2, WU Desheng2, HONG Wenxu3, LIU Jianjun2, KE Yuebin2, YUAN Jianhui2, LI Shuangfei1   

  1. 1. College of Life and Marine Science, Shenzhen University, Shenzhen 518060;
    2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055;
    3. Shenzhen Research Institute of Population and Family Planning, Shenzhen 518040, Guangdong, China
  • Received:2015-09-15 Revised:2015-10-25 Online:2015-11-30 Published:2015-11-30

摘要: 目的:研究纳米SiO2颗粒(SiO2-NPs)暴露下斑马鱼行为学的变化,并对其变化规律进行分析,以期对突发的SiO2-NPs污染实现早期的预警。方法:利用生物在线监测仪,以正常水质中斑马鱼的行为变化为基础,比较300、600、1200 mg/L SiO2-NPs暴露下斑马鱼的个体行为(包括游动速度、游动高度、转弯次数和加速度)和群体行为(包括平均距离和分散度)的变化规律。结果:与正常水质比较,600和1200 mg/L SiO2-NPs暴露下,斑马鱼的游动行为发生改变,游动速度在6 h时的降幅达到30%~50%,与暴露前相比差异显著(P均<0.01);游动高度的改变稍滞后于游动速度,在19 h时迅速下降,24 h时降幅达到90%以上(P<0.01);斑马鱼原有群体行为也发生改变,平均距离和分散度在24 h时均显著下降(P均<0.01)。结论:SiO2-NPs暴露下,斑马鱼的行为短时间内变化明显,且随着浓度的增加,变化越明显,基本符合环境压力模型。

关键词: 纳米SiO2颗粒, 斑马鱼, 行为学, 生物在线检测

Abstract: OBJECTIVE: To establish the early warning of the contamination of nano-SiO2 particle,swimming behavior changes of zebrafish under nano-SiO2 particle exposure were studied. METHODS: Based on behavior response of zebrafish,effects of nano-SiO2particles,including individual endpoints(swimming speed,swimming depth,turning frequency and acceleration) and group endpoints(distance and dispersion),were investigated by using Online Bio monitoring. RESULTS: Swimming behavior changes of zebrafish were affected significantly in 600 and 1200 mg/L exposure dose groups. Swimming speed was decreased 30%-50% 6 h after exposure. Changes of swimming depth lagged behind changes of swimming speed,which were decreased by more than 90% 24 h after exposure. Group behavior of zebrafish was also changed,distance and dispersion were significantly decreased(all P<0.01). CONCLUSION: After exposure,swimming behavior of zebrafish(swimming speed,swimming depth,turning frequency,distance and dispersion) were affected significantly wie increase of nano-SiO2 particles concentration,which is consistend wie Stepwise Stress Model.

Key words: nano-SiO2 particles, zebrafish, behavior, biological monitoring

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