含有机锡聚氯乙烯膜作为细胞毒性试验阳性对照材料的研究

doi:10.3969/j.issn.1004-616x.2015.06.016

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (6): 480-483.doi: 10.3969/j.issn.1004-616x.2015.06.016

含有机锡聚氯乙烯膜作为细胞毒性试验阳性对照材料的研究

付步芳¹, 林红赛², 王春仁¹, 冯晓明¹

1. 中国食品药品检定研究院, 北京 100050;
2. 北京市医疗器械检验所, 北京 101111

收稿日期:2015-06-15 修回日期:2015-10-20 出版日期:2015-11-30 发布日期:2015-11-30
作者简介:付步芳,E-mail:fbf377@163.com;林红赛,E-mail:lhs.7.17@163.com。
基金资助:
国家科技支撑计划重点专项(2012BA122B01)资助

Study of positive reference material for cytotoxicity test

FU Bufang¹, LIN Hongsai², WANG Chunren¹, FENG Xiaoming¹

1. National Institutes for Food and Drug Control, Beijing 100050;
2. Beijing Institute of Medical Device Testing, Beijing 101111, China

Received:2015-06-15 Revised:2015-10-20 Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 目的:以医用聚氯乙烯作为基质加入马来酸二丁基锡制备细胞毒性阳性对照材料。方法:对原材料进行鉴别和杂质检查后,采用溶剂流延法来制备含有机锡聚氯乙烯膜。制备样品浸提液,并用其处理L929细胞,通过观察细胞形态和MTT试验选择适合的有机锡浓度。并对有机锡聚氯乙烯膜的均匀性和稳定性进行考察。结果:溶剂流延法制备的含0.005%有机锡聚氯乙烯膜浸提液的细胞毒性与浸提液的浓度呈明显的相关性,具有毒性梯度。均匀性试验结果显示,各组浸提液中锡元素统计量F值=1.07,小于F临界值3.22。稳定性试验结果显示,50℃老化条件下,不同老化时间样品浸提出的锡元素含量与老化前比较差异均无统计学意义(P>0.05)。结论:含0.005%有机锡聚氯乙烯膜浸提液呈现稳定的细胞毒性反应,含有机锡聚氯乙烯膜的均匀性和稳定性良好,适宜作为细胞毒性阳性对照材料。

关键词: 对照材料, 细胞毒性试验, 聚氯乙烯, 有机锡

Abstract: OBJECTIVE: Polyvinyl chloride(PVC) was used as matrix to prepare the positive reference material by adding dibutyl tin maleate. METHODS: Identification and impurity inspection were carried out for the matrix. The positive reference material,PVC film containing organic tin,was prepared by tape casting process. Sample extract was prepared for L929 cell culture medium. The optimal organic tin concentration was determined by cell morphology examination and MTT test. Homogeneity and stability test were performed for the positive reference material. RESULTS: Extract of PVC film containing 0.005% organic tin exhibited significant cytotoxicity in a dose-effect manner. The homogeneity test showed the statisticd F was 1.07 which was less than the threshold value F. PVC film containing 0.005% organic tin exhibited stable positive reactions. Whilst the materials before and after aging test revealed that the tin contentobtained from extraction was uniform(P>0.05). CONCLUSION: Extract of PVC film containing 0.005% organic tin showed appropriate cytotoxicity,PVC film containing organic tin had a good uniformity and stability,so it could suitable as a cytotoxic reference material.

Key words: reference material, cytotoxicity test, polyvinyl chloride, organic tin

中图分类号:

R994.3

付步芳, 林红赛, 王春仁, 冯晓明. 含有机锡聚氯乙烯膜作为细胞毒性试验阳性对照材料的研究[J]. 癌变·畸变·突变, 2015, 27(6): 480-483.

FU Bufang, LIN Hongsai, WANG Chunren, FENG Xiaoming. Study of positive reference material for cytotoxicity test[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 480-483.

[1]	崔胜金, 陈泽衍, 曹朝鹏, 黄荣, 刘青, 周义文. 纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究[J]. 癌变·畸变·突变, 2017, 29(5): 374-378.
[2]	马茂才1;黄萍1;晏辉2;陶云1;邓雅斌 1;李东辉1. 纳米二氧化钛和纳米氧化锌的Ames试验[J]. , 2010, 22(4): 302-304.
[3]	彭国庆1;2;钟才高1;张琼2;关岚1;余剑英2;肖芳1. 甲醛对雌性大鼠卵巢储备功能的影响[J]. , 2010, 22(1): 32-0034.
[4]	武红叶(综述)；曾　明(审校). 六价铬致癌机制的研究进展[J]. , 2006, 18(6): 491-493.
[5]	王　敏;吕嘉春. 结晶型硫化镍诱发细胞恶变过程中端锚聚合酶mRNA的表达[J]. , 2006, 18(4): 314-315.

含有机锡聚氯乙烯膜作为细胞毒性试验阳性对照材料的研究

Study of positive reference material for cytotoxicity test

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价