Notch3增强三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性

doi:10.3969/j.issn.1004-616x.2016.02.006

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (2): 111-114,120.doi: 10.3969/j.issn.1004-616x.2016.02.006

Notch3增强三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性

吴华涛¹, 沈家欣^2,3, 刘静^2,3

1. 汕头大学医学院第一附属医院普通外科, 广东汕头 515041;
2. 汕头大学医学院长江学者实验室, 广东汕头 515041;
3. 广东省乳腺癌诊治研究重点实验室, 广东汕头 515041

收稿日期:2015-10-10 修回日期:2015-12-21 出版日期:2016-03-31 发布日期:2016-03-31
通讯作者: 刘静,E-mail:jliu12@stu.edu.cn E-mail:jliu12@stu.edu.cn
作者简介:吴华涛,E-mail:wuhuatao@126.com
基金资助:
国家自然科学基金(81501539);广东省自然科学基金(2015A030310211);广东省创新人才项目(2014KQNCX078)和人才培育项目;汕头市科技计划项目(汕府科[2014]62号)

Overexpression of Notch3 enhance the efficacy of cisplatin to MDA-MB-231

WU Huatao¹, SHEN Jiaxin^2,3, LIU Jing^2,3

1. Department of General Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041;
2. Changjiang Scholar's Laboratory, Shantou University Medical College, Shantou 515041;
3. Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Breast Cancer, Shantou 515041, Guangdong, China

Received:2015-10-10 Revised:2015-12-21 Online:2016-03-31 Published:2016-03-31

摘要/Abstract

摘要： 目的: 探讨干细胞相关因子Notch3在三阴性乳腺癌MDA-MB-231细胞对顺铂化疗敏感性中的作用。方法: 用Lipofectamine 2000试剂转染乳腺癌MDA-MB-231细胞并用G418筛选稳定转染细胞系,根据转染质粒不同分为MDA-MB-231-PCLE组(对照组)和MDA-MB-231-N3ICD组(过表达Notch3组),应用实时荧光定量PCR和Western blot方法检测不同组间Notch3表达的差异;用CCK8法检测各组MDA-MB-231细胞对顺铂敏感性的差异;同时利用Lipofectamine 2000转染Notch3的小干扰序列,根据转染序列不同分为T47D-NC(对照组)和T47D-siNotch3(Notch3敲减组);采用免疫印迹检测Notch3表达改变后凋亡相关因子caspase-3的表达情况。结果: 与对照组比较,高表达Notch3后三阴性乳腺癌对顺铂的敏感性增加(P<0.05),同时caspase-3的表达随着Notch3的增加而增加,而在高表达Notch3的乳腺癌T47D细胞中,敲减Notch3的表达使caspase-3表达减少。结论: 高表达Notch3可以增加三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性,从而增强患者化疗疗效。

关键词: Notch3, 顺铂, 乳腺癌, 化疗

Abstract: OBJECTIVE: To investigate the effect of stem cell-related factor, Notch3 on human triple negative breast cancer cell, MDA-MB-231 and its resistance to cisplatin, and explore if overexpressed Notch3 can enhance the sensitivity of resistance cells to cisplatin. METHODS: Lipofectamine 2000 was applied to transfect Notch3 plasmid into MDA-MB-231. These cells were divided into MDA-MB-231-PCLE group and MDA-MB-231-N3ICD group, according to the use of different plasmids. RT-PCR and Western blot methods were used to analyze the expression of Notch3 in different groups. The effects of cisplatin on cell proliferation were assessed by CCK8 assay and evaluated by GraphPad Prism 5. Lipofectamine 2000 was also applied to transfect the siNotch3 or scramble to cells and they were divided into T47D-NC and T47D-siNotch3 groups. The protein levels of apoptosis-related factor were detected using RT-PCR methods and Western blot. RESULTS: Then efficacy of cisplatin to triple negative breast cancer cells was enhanced by overexpression of Notch3. Apoptosis-related factor, caspase-3 was up-regulated by Notch3 overexpression. However, siNotch3 induced the downregulation of caspase-3 in T47D, in which the expression of Notch3 was high. CONCLUSION: Overexpression of Notch3 enhanced the sensitivity of MDA-MB-231 to cisplatin which suggests that this may improve the efficiency of chemotherapy in patients with breast cancer.

Key words: Notch3, cisplatin, breast cancer, chemotherapy

中图分类号:

吴华涛, 沈家欣, 刘静. Notch3增强三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性[J]. 癌变·畸变·突变, 2016, 28(2): 111-114,120.

WU Huatao, SHEN Jiaxin, LIU Jing. Overexpression of Notch3 enhance the efficacy of cisplatin to MDA-MB-231[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 111-114,120.

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Notch3增强三阴性乳腺癌MDA-MB-231细胞对顺铂的敏感性

Overexpression of Notch3 enhance the efficacy of cisplatin to MDA-MB-231

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