铅暴露工人外周血淋巴细胞遗传损伤与端粒长度的改变

doi:10.3969/j.issn.1004-616x.2016.03.004

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (3): 179-184.doi: 10.3969/j.issn.1004-616x.2016.03.004

铅暴露工人外周血淋巴细胞遗传损伤与端粒长度的改变

陆垚¹, 刘新霞², 邢秀梅¹, 赵志强¹, 欧晓燕¹, 苏晓琳¹, 孙易¹, 陈静莉¹, 申碧羚¹, 蒋骏¹, 何云¹

1. 中山大学公共卫生学院, 广东广州 510080;
2. 中山市疾病预防控制中心职业病防治所, 广东中山 528400

收稿日期:2016-03-04 修回日期:2016-04-05 出版日期:2016-05-31 发布日期:2016-05-31
通讯作者: 何云,E-mail:heyun7@mail.sysu.edu.cn E-mail:heyun7@mail.sysu.edu.cn
作者简介:陆垚,E-mail:lu409010@sina.com。
基金资助:
国家自然科学基金(81273097,81472998);中山大学"百人计划"引进人才科研启动基金(18801031)

DNA damage and telomere length changes in peripheral leukocytes from lead exposed workers

LU Yao¹, LIU Xinxia², XING Xiumei¹, ZHAO Zhiqiang¹, OU Xiaoyan¹, SU Xiaolin¹, SUN Yi¹, CHEN Jingli¹, SHEN Biling¹, JIANG Jun¹, HE Yun¹

1. School of Public Health, Sun Yat-sen University, Guangzhou 510080;
2. Prevention and Control Center for Occupational Diseases, Zhongshan Center for Disease Control and Prevention, Zhongshan 528400, Guangdong, China

Received:2016-03-04 Revised:2016-04-05 Online:2016-05-31 Published:2016-05-31

摘要/Abstract

摘要： 目的:探讨铅暴露工人外周血淋巴细胞遗传损伤情况与端粒长度改变之间的关系。方法:以珠三角某蓄电池厂的21名铅暴露工人为铅暴露组,26名无铅接触史的工人作为对照组。针对工人开展问卷调查并采集肝素抗凝外周血。检测工人外周血中血铅浓度、淋巴细胞的彗星试验指标、胞质分裂阻滞微核试验指标以及相对端粒长度改变情况。结果:铅暴露工人的血铅浓度为(3.08±1.86) mg/mL,与对照人群(0.26±0.25) mg/mL相比明显升高(P<0.05)。彗星试验中,铅暴露组工人的慧星尾长高于对照组(P<0.05);胞质分裂阻滞微核试验中,暴露组工人双核微核率和微核细胞率均高于对照组(P<0.05);铅暴露工人的外周血相对端粒长度较对照人群缩短(P<0.05)。相关分析结果显示,胞质阻滞微核试验的指标中双核微核率、微核细胞率、核桥率与工龄呈正相关(r_s分别为0.447、0.432、0.351,P均<0.05)。外周血相对端粒长度与工人工龄、血铅水平均呈负相关(r_s分别为-0.306、-0.394,P均<0.05)。结论:铅暴露可致工人外周血淋巴细胞的遗传损伤以及相对端粒长度的缩短。

关键词: 端粒长度, 遗传损伤, 铅暴露, 彗星试验, 胞质分裂阻滞微核试验

Abstract: OBJECTIVE:To investigate the induction of DNA damage and telomere length changes in peripheral leukocytes from lead exposed works in comparison with un-exposed controls.METHODS:21 lead exposed workers from a battery plant in the Pearl River Delta Region and 26 non-exposed workers were investigated. Questionnaires were surveyed. Blood samples and peripheral leukocytes were analyzed for blood lead concentrations, telomere length, Comet assay, and cytokinesis-block micronucleus assay. RESULTS:Blood lead concentration of exposed workers (3.08±1.86) mg/mL was significantly higher than that (0.26±0.25) mg/mL of the controls (P<0.05). In addition, tail lengthsand micronuclei frequencies in leukocytes from the exposed workers were significantly higher than that of the controls (P<0.05). However, relative telomere lengths from leukocytes of lead exposed workers were shorter than that of the controls(P<0.05). Correlation analyses indicated that micronucleus frequencies in binucleated cells, rate of micronucleated binucleate cells and rate of nucleoplasmic bridges were positively correlated with work age (P<0.05), and the rank correlation coefficient (rs) were 0.447, 0.432, 0.351 respectively. Work age and blood lead concentration were negatively correlated with relative telomere length of leukocytes (P<0.05), and rs were -0.306, -0.394 respectively. CONCLUSION:Lead exposure induced DNA damage, micronuclei and shorter telomere length in peripheral leukocytes of exposed workers.

Key words: telomere length, genetic damage, lead exposure, comet assay, cytokinesis-block micronucleus assay

中图分类号:

R994.6
Q355

陆垚, 刘新霞, 邢秀梅, 赵志强, 欧晓燕, 苏晓琳, 孙易, 陈静莉, 申碧羚, 蒋骏, 何云. 铅暴露工人外周血淋巴细胞遗传损伤与端粒长度的改变[J]. 癌变·畸变·突变, 2016, 28(3): 179-184.

LU Yao, LIU Xinxia, XING Xiumei, ZHAO Zhiqiang, OU Xiaoyan, SU Xiaolin, SUN Yi, CHEN Jingli, SHEN Biling, JIANG Jun, HE Yun. DNA damage and telomere length changes in peripheral leukocytes from lead exposed workers[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 179-184.

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铅暴露工人外周血淋巴细胞遗传损伤与端粒长度的改变

DNA damage and telomere length changes in peripheral leukocytes from lead exposed workers

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