关键词: 纳米氧化铜, CHL细胞, 细胞毒性

Abstract: OBJECTIVE:To study the cytotoxicity of CHL cells after treatment with nanometer copper-oxide. METHODS:CHL cells in different densities (1×10⁵, 5×10⁴, 2.5×10⁴and1.25×10⁴/mL) were monitored continuously by RTCA and their whole growth curves were evaluated. Then, the cytotoxicity of CHL cells treated by nanometer copper-oxide in different doses (0, 2, 1, 0.5, 0.25, 0.125, 0.0625 and 0.0313 mg/mL) was real-time monitored, and the accurate IC₅₀ value of the continuous time and the curve of IC₅₀ in continuous time were studied. RESULTS:Growth curves in cultures with different cell densities were measured, and cultures with 5×10⁴/mL inoculated cell number was found the best for toxic effect test. The starting time of the toxic effect after treatment with nano-copper oxide was about 4-5 h. At 6 h after treatment with nanometer copper-oxide, the IC₅₀ value was bigger with a large volatility, while it presented a dramatic decline after 6 h. The value of IC₅₀ after 12, 24, 36 and 48 h was 0.1570, 0.0511, 0.0429, 0.0168 mg/mL, respectively. CONCLUSION:RTCA was useful for detecting cytotoxicity of nanometer copper-oxide, considering the unique characteristics of nanomaterials. In addition, cytotoxicity at 4-18 h after treatment should be investigated further in the future.

Key words: nanometer copper-oxide, CHL cells, cytotoxicity