原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响

doi:10.3969/j.issn.1004-616x.2016.03.011

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (3): 214-217.doi: 10.3969/j.issn.1004-616x.2016.03.011

原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响

陈金枝^1,2, 张小强^1,2, 曲志华^1,2, 周亚盼^1,2, 张妍^1,2, 梁晓瑜^1,2

1. 东南大学公共卫生学院, 江苏南京 210009;
2. 东南大学环境医学工程教育部重点实验室, 江苏南京 210009

收稿日期:2015-12-29 修回日期:2016-03-11 出版日期:2016-05-31 发布日期:2016-05-31
通讯作者: 张小强,E-mail:zhangxq7843@126.com E-mail:zhangxq7843@126.com
作者简介:陈金枝,E-mail:chenjinzhi1990@sina.com。
基金资助:
中央高校基本科研业务费专项资金资助和江苏省普通高校研究生科研创新计划资助项目资助(SJZZ_0032);江苏省卫生厅预防医学科研基金(Y2013070)

Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide

CHEN Jinzhi^1,2, ZHANG Xiaoqiang^1,2, QU Zhihua^1,2, ZHOU Yapan^1,2, ZHANG Yan^1,2, LIANG Xiaoyu^1,2

1. School of Public Health, Southeast University, Nanjing 210009;
2. Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University, Nanjing 210009, Jiangsu, China

Received:2015-12-29 Revised:2016-03-11 Online:2016-05-31 Published:2016-05-31

摘要/Abstract

摘要： 目的:探讨原花青素对脂多糖(LPS)激活小鼠小胶质细胞(BV2)炎症介质分泌的影响。方法:以LPS激活BV2细胞构建神经炎症模型。分别采用0.1、0.5、1.0、5.0 μg/mL原花青素预处理后,1.0 μg/mL的LPS刺激BV2细胞24 h。采用MTT法检测细胞存活率;Griess法检测BV2细胞培养液上清中一氧化氮(NO)水平;ELISA法检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的分泌水平。结果:在实验剂量范围内,原花青素及LPS对BV2细胞活性均无显著影响(P>0.05)。但1.0 μg/mL LPS可引起BV2细胞各炎症因子NO、TNF-α、IL-1β和IL-6水平明显升高(P<0.05)。与LPS组相比,原花青素(0.1、0.5、1.0、5.0 μg/mL)预处理能使激活的BV2细胞培养液上清中NO释放量减少(P<0.05),TNF-α、IL-1β和IL-6含量降低(P<0.05),抑制作用呈剂量-效应关系。结论:在体外实验中,原花青素对LPS诱导小胶质细胞所致的炎症反应具有保护作用。

关键词: 原花青素, BV2细胞, 脂多糖, 神经炎症

Abstract: OBJECTIVE:To investigate the effects of pro-anthocyanidin on inhibiting inflammatory mediator secretion in LPS activation of BV2 microglia. METHODS:Neuroinflammation model was established by LPS-activated BV2 microglia.BV2 microglial were pretreated with different concentrations of pro-anthocyanidin (0.1, 0.5, 1.0, 5.0 μg/mL) and stimulated with LPS (1.0 μg/mL) for 24 h. Cell viability was assessed by MTT assay. Griess method was utilized to detect the content of NO. The secretion level of pro-inflammatory cytokines TNF-alpha, IL-1beta and IL-6 was examined using specific ELISA kits. RESULTS:Within a certain dosage ranges of pro-anthocyanidin and LPS, there was no negative effect on cell viability (P>0.05). LPS (1.0 μg/mL) significantly increased the release of inflammatory mediator nitric oxide (NO) as well as the cytokines TNF-α, IL-1β and IL-6(P<0.05). Compared with the LPS group, different concentrations of pro-anthocyanidin (0.1, 0.5, 1.0, 5.0 μg/mL) attenuated the release of NO, TNF-α, IL-1β and IL-6 in cells that were cultured in conditioned media from LPS-induced BV2 microglia. The effect was dose-dependent (P<0.05). CONCLUSION:We showed that pro-anthocyanidin has a protective effect on inflammatory responses of LPS-activated BV2 microglia in vitro.

Key words: proanthocyanidin, BV2 microglia, lipopolysaccharide, neuroinflammation

中图分类号:

R994.6

陈金枝, 张小强, 曲志华, 周亚盼, 张妍, 梁晓瑜. 原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响[J]. 癌变·畸变·突变, 2016, 28(3): 214-217.

CHEN Jinzhi, ZHANG Xiaoqiang, QU Zhihua, ZHOU Yapan, ZHANG Yan, LIANG Xiaoyu. Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 214-217.

参考文献

[1] Goldmann T,Prinz M. Role of microglia in CNS autoimmunity[J]. Clin Dev Immunol,2013,2013(1):80-93.
[2] Tsuda M,Tozaki-Saitoh H,Inoue K. Purinergic system,microglia and neuropathic pain[J]. Curr Opin Pharmacol,2012,12(1):74-79.
[3] Lehnardt S. Innate immunity and neuroinflammation in the CNS:the role of microglia in Toll-like receptor-mediated neuronal injury[J]. Glia,2010,58(3):253-263.
[4] Mao SS,Hua R,Zhao XP,et al. Exogenous administration of PACAP alleviates traumatic brain injury in rats through a mechanism involving the TLR4/MyD88/NF-κB pathway[J]. J Neurotrauma,2012,29(10):1941-1959.
[5] Aravalli RN,Peterson PK,Lokensgard JR. Toll-like receptors in defense and damage of the central nervous system[J]. J Neuroimmune Pharm,2007,2(4):297-312.
[6] Hughes V. Microglia:the constant gardeners[J]. Nature,2012,485(7400):570-572.
[7] Cos P,Bruyne TD,Hermans N,et al. Proanthocyanidins in health care:current and new trends[J]. Curr Med Chem,2004,11(10):1345-1359.
[8] Yin W,Li B,Li X,et al. Anti-inflammatory effects of grape seed procyanidin B2 on a diabetic pancreas[J]. Food Funct,2015,6(9):3065-3071.
[9] Strathearn KE,Yousef GG,Grace MH,et al. Neuroprotective effects of anthocyanin- and proanthocyanidin-rich extracts in cellular models of Parkinsons disease[J]. Brain Res,2014,1555(1):60-77.
[10] Dai XJ,Li N,Yu L,et al. Activation of BV2 microglia by lipopolysaccharide triggers an inflammatory reaction in PC12 cell apoptosis through a toll-like receptor 4-dependent pathway[J]. Cell Stress Chaperones,2015,20(2):321-331.
[11] Phani S,Loike JD,Przedborski S. Neurodegeneration and inflammation in Parkinson's disease[J]. Parkinsonism Relat Disord,2012,18(Sup 1):207-209.
[12] Blasi E,Barluzzi R,Bocchini V,et al. Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus[J]. J Neuroimmunol,1990,27(2/3):229-237.
[13] Henn A,Lund S,Hedtjarn M,et al. The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation[J]. Altex-Altern Tierexp,2009,26(2):83-94.
[14] Dilshara MG,Lee KT,Choi YH,et al. Potential chemoprevention of LPS-stimulated nitric oxide and prostaglandin E2 production by α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-3-indolecarbonate in BV2 microglial cells through suppression of the ROS/PI3K/Akt/NF-κB pathway[J]. Neurochem Int,2014,67(1):39-45.
[15] Gao X,Cassidy A,Schwarzschild MA,et al. Habitual intake of dietary flavonoids and risk of Parkinson disease[J]. Neurology,2012,78(1):1138-1145.
[16] Kraft AD,Harry GJ. Features of microglia and neuro-inflammation relevant to environmental exposure and neurotoxicity[J]. Int J Environ Res Public Health,2011,8(7):2980-3018.
[17] Asha Devi S,Sagar Chandrasekar BK,Manjula KR,et al. Grape seed proanthocyanidin lowers brain oxidative stress in adult and middle-aged rats[J]. Exp Gerontol,2011,46(11):958-964.
[18] Xu Y,Li S,Chen R,et al. Antidepressant-like effect of low molecular proanthocyanidin in mice:involvement of mono-aminergic system[J]. Pharmacol Biochem Behav,2010,94(3):447-453.

原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响

Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	尹亚萍, 王宇, 田雨阳, 马焜, 李百祥. 褪黑素在帕金森病治疗中的研究进展[J]. 癌变·畸变·突变, 2022, 34(1): 75-78,81.
[2]	王旭, 张小强, 刘静, 陈姚姚, 卢晓星, 苗歆雨. 原花青素对Aβ_25-35介导的tau蛋白过度磷酸化及p38MAPK信号通路的影响[J]. 癌变·畸变·突变, 2019, 31(2): 96-101,110.
[3]	张妍, 张小强, 梁晓瑜, 王旭, 刘静. 原花青素抑制脂多糖激活神经小胶质细胞的机制[J]. 癌变·畸变·突变, 2018, 30(2): 98-102.
[4]	周亚盼, 张小强, 曲志华, 张妍, 梁晓瑜. 原花青素对淀粉样蛋白Aβ_25-35诱导的SH-SY5Y细胞毒性的影响[J]. 癌变·畸变·突变, 2017, 29(2): 91-95.
[5]	张敏辉, 周珏, 刘冉, 李晓波, 梁戈玉, 李云晖. 微囊藻毒素-LR所致神经小胶质细胞BV-2的炎性反应[J]. 癌变·畸变·突变, 2015, 27(5): 375-377,382.
[6]	苏芸1,谢泽锋2 ,辛岗1,许燕璇1,李康生1，*. 脂多糖预刺激对大脑皮质星形胶质细胞和小胶质细胞NO分泌水平的影响[J]. , 2011, 23(3): 176-180.
[7]	段玉清1,2/ 张海晖1/ 吴　佳2/ 谢笔钧2. 莲房原花青素对黑色素瘤B16细胞的诱导分化作用[J]. , 2007, 19(5): 377-380.
[8]	张　美/李　慧/罗红军/罗文鸿. 脂多糖诱导急性肺损伤过程中SSAO酶的活性变化[J]. , 2007, 19(3): 223-227.
[9]	隋　萍，刘海珍，钟进义. 葡多酚对骨髓细胞Bcl-2和Bax表达的影响[J]. , 2004, 16(4): 206-207.
[10]	孙志广1, 赵万洲2, 陆　茵2, 唐玲芳3, 张珍玲3, 张世玮2. 葡萄籽原花青素对鼠伤寒沙门氏菌的抗诱变作用[J]. , 2002, 14(3): 191-194.