小窝蛋白-1和E-钙黏蛋白在食管癌TE1细胞系中的表达及其意义

doi:10.3969/j.issn.1004-616x.2016.03.014

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (3): 227-230.doi: 10.3969/j.issn.1004-616x.2016.03.014

小窝蛋白-1和E-钙黏蛋白在食管癌TE1细胞系中的表达及其意义

蔡晓曼, 陈素钻, 俞晶, 刘朝晖, 吴瑞暖, 汤盛新, 郭光华

汕头大学医学院第一附属医院消化内科, 广东汕头 515041

收稿日期:2015-12-07 修回日期:2016-04-10 出版日期:2016-05-31 发布日期:2016-05-31
通讯作者: 郭光华,E-mail:ghguo@stu.edu.cn E-mail:ghguo@stu.edu.cn
作者简介:蔡晓曼,E-mail:794899509@qq.com。

The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1

CAI Xiaoman, CHEN Suzuan, YU Jing, LIU Zhaohui, WU Ruinuan, TANG Shengxin, GUO Guanghua

Department of Gastroenterology, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2015-12-07 Revised:2016-04-10 Online:2016-05-31 Published:2016-05-31

摘要/Abstract

摘要： 目的:检测小窝蛋白-1(Cav-1)和E-钙黏蛋白(E-cad)在食管癌TE1细胞系中的表达水平,探讨Cav-1在食管癌转移中可能的作用及机制。方法:化学合成针对Cav-1基因3个不同靶点的siRNA(Cav-1 siRNA1,Cav-1 siRNA2和对照siRNA),分别转染食管癌TE1细胞系,以未转染细胞为空白对照,转染对照siRNA细胞为阴性对照。采用Western blot法检测RNA干扰后TE1细胞中Cav-1和E-cad的表达情况;Transwell方法检测各组细胞的迁移和侵袭能力。结果:Western blot检测结果提示,与空白对照组比较,Cav-1 siRNA使食管癌TE1细胞系中Cav-1表达水平降低(P<0.01),E-cad表达水平升高(P<0.01);Transwell迁移及侵袭试验结果提示转染Cav-1 siRNA后细胞的迁移、侵袭能力较空白对照组显著减弱(P<0.05)。结论:Cav-1可能通过调节E-cad的表达影响食管癌的转移。

关键词: 小窝蛋白-1, E-钙黏蛋白, 食管癌, TE1细胞系

Abstract: OBJECTIVE: To detect the expression of Caveolin-1(Cav-1) and E-cadherin(E-cad) in esophageal carcinoma TE1 cell line and to explore the involvement of Cav-1 in metastasis of esophageal carcinoma. METHODS:Small interfering RNAs Cav-1 siRNA1 and Cav-1 siRNA2 which targeted Cav-1 and negative control siRNA were individually transfected into esophageal carcinoma TE1 cell line. Non-transfected cells were used as blank control. Expressions of Cav-1 and E-cad were detected in cell extracts using Western blot. Migration and invasion ability of cells were evaluated using the Transwell assay. RESULTS:The Western blot analysis showed that Cav-1 siRNAs effectively suppressed the expression of Cav-1 and up-regulated the expression of E-cad in TE1 cells. The transwell assay showed that the migration and invasion ability of Cav-1 siRNA treated group were significantly weakened compared with the blank control group (P<0.05). CONCLUSION:Cav-1 may affect metastasis of esophageal carcinoma by up-regulating the expression of E-cad.

Key words: caveolin-1, E-cadherin, esophageal carcinoma, TE1 cell line

中图分类号:

R735.1

蔡晓曼, 陈素钻, 俞晶, 刘朝晖, 吴瑞暖, 汤盛新, 郭光华. 小窝蛋白-1和E-钙黏蛋白在食管癌TE1细胞系中的表达及其意义[J]. 癌变·畸变·突变, 2016, 28(3): 227-230.

CAI Xiaoman, CHEN Suzuan, YU Jing, LIU Zhaohui, WU Ruinuan, TANG Shengxin, GUO Guanghua. The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 227-230.

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小窝蛋白-1和E-钙黏蛋白在食管癌TE1细胞系中的表达及其意义

The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1

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