食管癌干细胞相关标记物及其分离和鉴定的研究进展

doi:10.3969/j.issn.1004-616x.2016.03.019

摘要/Abstract

摘要： 食管癌是严重危害人类健康的一种恶性肿瘤,由于早期症状不明显和缺乏简单有效的诊断手段,食管癌患者在确诊时多已处于晚期,治疗效果不甚理想,其死亡率一直位于消化系统肿瘤前列。肿瘤干细胞的理论引导我们从新的角度认识食管癌。全面了解食管癌干细胞的特异标记物、分离纯化手段和生物学性状可能为攻克食管癌这一世界性难题带来新的方向,而食管癌干细胞的分离鉴定是对其进一步研究的基础。本文就食管癌干细胞相关标记物、分离鉴定的研究进展作一综述,并对其未来的发展趋势进行了展望。

关键词: 肿瘤干细胞, 食管癌, 分选, 鉴定

中图分类号:

R735.1

郑鑫林, 夏学阳, 张金周, 张建华, 李斌. 食管癌干细胞相关标记物及其分离和鉴定的研究进展[J]. 癌变·畸变·突变, 2016, 28(3): 246-249.

参考文献

[1] Torre LA,Bray F,Siegel RL,et al. Global cancer statistics,2012[J]. Ca Cancer J Clin,2015,65(2):87-108.
[2] 钟钏,谭家驹,徐致祥. 食管癌流行病学病因学研究进展[J]. 河南预防医学杂志,2011,22(1):1-17.
[3] Ogawa K,Yoshioka Y,Isohashi F,et al. Radiotherapy targeting cancer stem cells:current views and future perspectives[J]. Anticancer Res,2013,33(3):747-754.
[4] Chen J,Li Y,Yu TS,et al. A restricted cell population propagates glioblastoma growth after chemotherapy[J]. Nature,2012,488(7412):522-526.
[5] Yang YM,Chang JW. Current status and issues in cancer stem cell study[J]. Cancer Invest,2008,26(7):741-755.
[6] Huang DZ,Gao QL,Guo LP,et al. Isoltaion and identification of cancer stem-like cells in esophageal carcinoma cell lines[J]. Stem Cells Dev,2009,18(3):465-473.
[7] Liu L,Zuo LF,Guo JW. ABCG-2 gene amplification and expression in esophageal cancer cells with acquired adriamycin resistance[J]. Mol Med Rep,2014,9(4):1299-1304.
[8] Tsunoda S,Okumura T,Ito T,et al. ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma[J]. Oncology,2006,71(3/4):251-258.
[9] Wang JL,Yu JP,Sun ZQ,et al. Radiobiological characteristics of cancer stem cells from esophageal cancer cell lines[J]. World J Gastroenterol,2014,20(48):18296-18305.
[10] 郑淑妍,陈强,黄小萍,等. CD44及CD44V6在不同分化程度食管癌中的表达水平[J]. 分子诊断与治疗杂志,2013,5(4):232-235.
[11] Honing J,Pavlov KV,Mul VE,el at. CD44,SHH and SOX2 as novel biomarkers in esophageal cancer patients treated with neoadjuvant chemoradiotherapy[J]. Radiother Oncol,2015,117(1):152-158.
[12] Huang SD,Yuan Y,Liu XH,et al. Self-renewal and chemotherapy resistance of side of P75NTR positive cells in esophageal squamous[J]. BMC Cancer,2009,9(9):9-17.
[13] 孙志刚,黄盛东,张宝仁,等. 应用P75NTR分选食管肿瘤干细胞并鉴定其生物学特性[J]. 第二军医大学学报,2009,30(5):480-486.
[14] Li S,Yue D,Chen X,et al. Epigenetic regulation of CD271,a potential cancer stem cell marker associated with chemoresistance and metastatic capacity[J]. Oncol Rep,2015,33(1):425-432.
[15] Wang JF,Liu Y,Liu WJ,et al. Expression of Bmi-1 gene in esophageal carcinoma cell EC9706 and its effect on cell cycle, apoptosis and migration[J]. Chin J Cancer,2010,29(7):689-696.
[16] Zhang Y,Zhang YL,Chen HM,et al. Expression of Bmi-1 and PAL-1 in esophageal squamous cell carcinoma[J]. World J Gastroenterol, 2014,20(18):5533-5539.
[17] 黄燕燕,赵生霞,李秋霞,等. 人食管鳞癌Eca109细胞株中肿瘤干细胞的分离鉴定[J]. 石河子大学学报(医学版),2014,32(3):335-339.
[18] Vaiphei K,Sinha SK,Kochhar R. Comparative analysis of Oct4 in different histological subtypes of esophageal squamous cell carcinomas in different clinical conditions[J]. Asian Pac J Cancer Prev,2014, 15(8):3519-3524.
[19] Li C,Yan Y,Ji W,et al. OCT4 positive regulates survivin expression to promote cell proliferation and leads to poor prognosis in esophageal squamous cell carcinoma[J]. PLoS One,2012,1(11):49693.
[20] 苏华芳,万秋燕,邹燕,等. 具干细胞特性食管癌耐放射细胞株基因表达分析[J]. 肿瘤学杂志,2010,16(1):40-45.
[21] Wang Y,Zhe H,Gao P,et al. Cancer stem cell marker ALDH1 experession is associated with lymph node metastasis and poor survival in esophageal squamous cell carcinoma:a study from high incidence area of northern China[J]. Dis Esophagus,2012,25(6):560-565.
[22] Ajani JA,Wang X,Song S,et al. ALDH-1 expression levels predict response or resistance to preoperative chemoradiation in resectable esophageal cancer patients[J]. Mol Oncol,2014,8(1):142-149.
[23] Tanaka M,Kijima H,Shimada H,et al. Expression of podoplanin and vimentin is correlated with prognosis in esophageal squamous cell carcinoma[J]. Mol Med Rep,2015,12(3):4029-4036.
[24] Lu C,Xu F,Gu J,et al. Clinical and biological significance of stem-like CD133⁺ CXCR4⁺ cells in esophageal squamous cell carcinoma[J]. J Thorac Cardiovasc Surg,2015,150(2):386-395.
[25] Sui P,Hu P,Zhang T,et al. High expression of CXCR-2 correlates with lymph node metastasis and predicts unfavorable prognosis in resected esophageal carcinoma[J]. Med Oncol,2014,31(2):809.
[26] 邓小峰,王晓博,熊力,等. 胆管癌干细胞的研究进展[J]. 中国普通外科杂志,2015,2(2):265-269.
[27] Zhao Y,Bao Q,Schwarz B,et al. Stem cell like side populations in esophageal cancer:a source of chemotherapy resistence and metastases[J]. Stem Cells Dev,2014,23(2):180-192.
[28] Chen YL,Li D,Wang DP,et al. Quiescence and attenuated DNA damage response promote survival of esophageal cancer stem cells[J]. J Cell Biochem,2013,113(12):3643-3652.
[29] 李步强,慕晓玲. 食管肿瘤干细胞的研究进展[J]. 中国现代医药杂志,2011,13(3):124-125.
[30] 崔翔,杨浪,于茜,等. 人食管鳞癌细胞系EC109中肿瘤干细胞的分离(富集)与鉴定[J]. 成都医学院学报,2012,7(3):378-382.
[31] Che SM,Zhang XZ,Liu XL,et al. The radiosensitization effect of NS398 on esophaeal cancer stem cell-like radioresistant cells[J]. Dis Esophagus,2011,24(4):265-273.
[32] 冉玉格. 人食管鳞癌放射抗性细胞系TE13R120的放射抗性与上皮间皮转化关系的研究[D]. 河北:河北医科大学,2014.
[33] 杨延灵,薛晓英,冉玉格,等. 反复照射食管癌放射抗性细胞系方法的重复性和稳定性研究[J]. 河北医科大学学报,2015,36(3):300-303.
[34] 苏成海,法逸华,许玉杰,等. 低剂量率γ射线杀伤肿瘤细胞机制的实验研究[J]. 核医学,2006,5(5):362-367.
[35] 汪建林,孙志强,于静萍,等. 食管癌细胞株中肿瘤干细胞样细胞的分离培养及生物鉴定[J]. 南京大学学报(自然科学版),2013, 33(10):1362-1367.
[36] 李珂,龙辉,王洁莲,等. 钼对人食管癌细胞ECA-109的化疗增敏作用及对食管癌干细胞P75NTR的影响[J]. 世界华人杂志,2012,20(11):959-963.
[37] 陈琦,吴清明,程静,等. 冬凌草甲素对食管癌干细胞放射增敏作用的研究[J]. 中国现代医学杂志,2012,22(29):57-60.
[38] 刘月姣,李晓敏. 提高食管癌放疗疗效的方法综述[J]. 基层医学论坛,2015,19(7):979-980.
[39] 张建华,宋铁牛,李斌,等. 艰难梭菌A毒素对食管癌干细胞损伤的研究[J]. 中国现代医学杂志,2013,23(25):16-19.
[40] Huang S,Li XQ,Chen X,et al. Inhibition of microRNA-21 increases radiosensitivity of esophageal cancer cells through phosphatase and tensin homolog deleted on chromosome 10 activation[J]. Dis Esophagus, 2013,26(8):823-831.
[41] 赫捷,邵康. 中国食管癌流行病学现状、诊疗现状及未来对策[J]. 中国癌症杂志,2011,27(7):501-504.