癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (4): 302-305.doi: 10.3969/j.issn.1004-616x.2016.04.011

• 检测研究 • 上一篇    下一篇

紫荷植物固体饮料的急性毒性和遗传毒性试验研究

罗雪婷1,2, 蔡绮君1, 赵博1, 陈伟民1, 陈晓1, 张晓文1   

  1. 1. 广州市体育科学研究所, 广东 广州 510650;
    2. 中山大学公共卫生学院, 广东 广州 510610
  • 收稿日期:2016-03-28 修回日期:2016-06-29 出版日期:2016-07-31 发布日期:2016-07-31
  • 通讯作者: 张晓文,E-mail:Zhang-xiao-wen@163.com E-mail:Zhang-xiao-wen@163.com
  • 作者简介:罗雪婷,E-mail:371090912@qq.com
  • 基金资助:
    广州市科技计划项目(2011J4300076)

Evaluation of acute and genetic toxicity of Zihe herbal solid beverage in mice

LUO Xueting1,2, CAI Qijun1, ZHAO Bo1, CHEN Weimin1, CHEN Xiao1, ZHANG Xiaowen1   

  1. 1. Guangzhou Institute of Sports Science, Guangzhou 510650;
    2. School of Public Health, Sun Yat-Sen University, Guangzhou 510610, Guangdong, China
  • Received:2016-03-28 Revised:2016-06-29 Online:2016-07-31 Published:2016-07-31

摘要: 目的:对紫荷植物固体饮料的急性毒性和遗传毒性进行评价。方法:急性毒性试验,采用限量法,设雌雄2组,每组10只SPF级昆明小鼠,累积剂量为10 g/(kg·d)。Ames试验,采用平板掺入法,设5000、1000、200、40、8μg/皿共5个剂量组;小鼠精原细胞染色体畸变试验,设2000、1000、500 mg/(kg·d)剂量组;哺乳动物红细胞微核试验,设2000、1000、500 mg/(kg·d)剂量组。3个遗传毒性试验均另设置阴性对照组和阳性对照组。结果:在14 d观察期内受试动物未见任何急性毒性反应,根据急性毒性分级,属于实际无毒级。Ames试验结果显示,该固体饮料属无致突变性。小鼠精原细胞染色体畸变试验结果显示,各剂量组小鼠染色体畸变率(0~1.6%),与阴性对照组(0.6%)比较,差异无统计学意义(P>0.05)。哺乳动物红细胞微核试验结果显示,各剂量组雌雄性小鼠骨髓嗜多染红细胞微核率与阴性对照组比较,差异无统计学意义(P>0.05)。3项遗传毒性试验结果显示该固体饮料未见遗传毒性。结论:在本实验条件下,紫荷植物固体饮料无明显急性毒性反应和遗传毒性。

关键词: 紫荷植物固体饮料, 急性毒性, 遗传毒性, 染色体畸变, Ames试验

Abstract: OBJECTIVE: To investigate acute and genetic toxicity of Zihe herbal solid beverage in mice. METHODS: Foracute oral toxicity test, male and female mice (10 mice per group) were orally administered a cumulative dose is 10 g/(kg·d) for the determination of mortality, LD50 and maximum tolerated dose. Three assays were used for determination of genetic toxicity:Ames test (using tablet adding method with 5 000, 1 000, 200, 40 and 8 μg/plate);spermatogonia chromosome aberration test[2000, 1000 and 500 mg/(kg·d) dose groups];erythrocytes micronucleus test[2000, 1000 and 500 mg/(kg·d) dose groups]. Moreover, negative control and positive control groups were used for the 3 genotoxicity tests. RESULTS: After 14 days of observation, no acute toxicity effect was observed in the exposed mice. From the Ames tests, the number of revertant colonies of TA97, TA98, TA100, TA102 strains were not significantly different compared with those from the negative control group. Chromosome aberration test showed that the sperm abnormality rate was 0-1.6% which was not significantly different compared with the control group (0.6%, P>0.05). The mammalian erythrocytes micronucleus test showed that there was no significant difference compared with the control group (P>0.05). CONCLUSION: Under our experimental conditions, the Zihe herbal solid beverage did not induce any obvious acute or genetic toxicity in mice.

Key words: Zihe herbal solid beverage, acute toxicity, genetic toxicity, chromosome aberration test, Ames test

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