癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (3): 222-225,234.doi: 10.3969/j.issn.1004-616x.2017.03.012

• 论著 • 上一篇    下一篇

外周血白细胞端粒相对长度与噪声性听力损失的相关性研究

刘新霞1,3, 陆垚2, 刘浩中1,3, 何云2,3   

  1. 1.中山市疾病预防控制中心, 广东 中山 528403;
    2.中山大学公共卫生学院预防医学系, 广东 广州 510080;
    3.中山大学公共卫生学院中山研究院, 广东 中山 528403
  • 收稿日期:2017-03-06 修回日期:2017-05-05 出版日期:2017-05-31 发布日期:2017-05-31
  • 通讯作者: 何云,E-mail:heyun_jane@163.com E-mail:heyun_jane@163.com
  • 作者简介:刘新霞,E-mail:994794966@qq.com。
  • 基金资助:
    国家自然科学基金(81273097,81472998);中山市科技计划项目医疗卫生重大科技专项(2015B1009)

Correlation between telomere length of peripheral blood leukocytes and noise-induced hearing loss

LIU Xinxia1,3, LU Yao2, LIU Haozhong1,3, HE Yun2,3   

  1. 1. Zhongshan Center for Disease Control and Prevention, Zhongshan 528403;
    2. Faculty of Preventive Medicine, School of Public Health, Sun Yat-sen University, Guangzhou 510080;
    3. Zhongshan Institute of the School of Public Health, Sun Yat-sen University, Zhongshan 528403, Guangdong, China
  • Received:2017-03-06 Revised:2017-05-05 Online:2017-05-31 Published:2017-05-31

摘要: 目的:探讨外周血白细胞端粒相对长度与噪声性听力损失(NIHL)的相关性。方法:用SV104IS无线个体噪声剂量计对某电脑机箱外壳制造企业所有作业岗位进行噪声强度检测,选择噪声强度超标[LEX,8 h ≥ 85 dB(A)]生产岗位的全体生产工人作为研究对象。采用问卷调查法获得研究对象的一般情况和职业史,实时荧光定量PCR检测研究对象外周血白细胞端粒相对长度,有序多分类Logistic回归法分析外周血白细胞端粒相对长度与噪声性听力损失(NIHL)的相关性。结果:该企业噪声超标作业岗位噪声强度(LEX,8 h)均值为(91.30±3.22) dB(A)。本次研究共调查127名噪声作业者:双耳听力正常43人(33.86%),双耳高频听阈提高75人(59.06%),单耳或双耳语频听阈提高伴双耳高频提高9人(7.09%)。不同NIHL程度工人的端粒相对长度差异有统计学意义(P < 0.05)。有序多分类Logistic回归分析显示,噪声强度、年龄、端粒相对长度、性别是NIHL的影响因素(P < 0.05)。结论:外周血白细胞端粒长度可能是发生NIHL的易感性生物标志物。

关键词: 外周血白细胞, 端粒, 相对长度, 噪声性听力损失

Abstract: OBJECTIVE:To study the correlation between telomere length of peripheral blood leukocytes and noise-induced hearing loss. METHODS:In a factory that produced computer shells,all posts that had noise intensity LEX,8 h ≥ 85 dB(A),as determined by SV104IS wireless noise intensity detector,were selected. Questionnaires were used for collecting general and occupational information from workers and real-time polymerase chain reaction was used to detect telomere length of their peripheral blood leukocytes. Ordinal ploytomous logistic regression was used for analyzing the correlation between telomere length and noise-induced hearing loss. RESULTS:The average noise intensity among LEX,8 h of overproof posts in the factory was (91.30±3.22) dB(A). This study recruited 127 noise-exposed workers consisting of 43 normal hearing workers (33.86%),75 abnormal high-frequency hearing loss workers (59.06%),9 abnormal speech-frequency hearing loss accompanied with abnormal high-frequency hearing loss (7.09%). Statistical significance was found in relative telomere length between different levels of noise-induced hearing loss (P < 0.05). It was revealed that noise intensity,age,gender and relative telomere length were factors related to noise-induced hearing loss (P < 0.05). CONCLUSION:Relative telomere length of peripheral blood leukocytes can potentially be used as a biomarker of susceptibility for noise-induced hearing loss.

Key words: peripheral blood leukocyte, telomere, relative length, noise-induced hearing loss

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