LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2017.04.012

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (4): 304-308,312.doi: 10.3969/j.issn.1004-616x.2017.04.012

LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义

李幼梅¹, 张思仪², 赵连梅², 单保恩², 张超³

1. 河北医科大学第四医院放疗三科, 河北石家庄 050011;
2. 河北医科大学第四医院科研中心, 河北石家庄 050011;
3. 河北医科大学第四医院检验科, 河北石家庄 050011

收稿日期:2016-11-11 修回日期:2017-05-08 出版日期:2017-07-31 发布日期:2017-07-31
通讯作者: 张超,E-mail:kyzxzc@163.com E-mail:kyzxzc@163.com
作者简介:李幼梅,E-mail:hbydsylym@163.com。
基金资助:
国家自然科学青年基金项目（81502032）

Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer

LI Youmei¹, ZHANG Siyi², ZHAO Lianmei², SHAN Baoen², ZHANG Chao³

1. The 3rd Department of Radiotherapy, The Fourth Hospital Affiliated to Hebei Medical Universiy, Shijiazhuang 050011;
2. Research Center, The Fourth Hospital Affiliated to Hebei Medical Universiy, Shijiazhuang 050011;
3. Clinical Laboratory, The Fourth Hospital Affiliated to Hebei Medical Universiy, Shijiazhuang 050011, Hebei, China

Received:2016-11-11 Revised:2017-05-08 Online:2017-07-31 Published:2017-07-31

摘要/Abstract

摘要：

目的：研究RNA结合蛋白LIN28A、上皮间质转化相关因子E-cadherin和vimentin在浸润性乳腺癌中的表达及三者的相关性，探讨其对乳腺癌进展的影响。方法：收集40例浸润性乳腺癌组织标本及其对应的癌旁组织，实时荧光定量PCR（qPCR）和免疫组织化学法分别检测LIN28A、E-cadherin和vimentin的mRNA和蛋白的表达，分析其与临床病理指标之间的关系，TUNNEL法检测LIN28A阳性癌组织中的细胞凋亡情况，统计分析LIN28A和EMT相关蛋白之间的相关性及其与乳腺癌患者生存率之间的关系。结果：qPCR结果显示，LIN28A mRNA在乳腺癌组织中的相对表达水平高于癌旁组织，其与E-cadherin mRNA的表达水平呈负相关，与vimentin mRNA呈正相关（r依次=-0.340和0.380，P均 < 0.05）。免疫组织化学检测结果显示，在乳腺癌组织中，LIN28A、E-cadherin和vimentin蛋白表达率分别为60%、55%和65%。LIN28A蛋白的表达与E-cadherin蛋白呈负相关（r依次=-0.533和0.364，P均 < 0.05），与vimentin蛋白呈正相关。LIN28A蛋白表达与肿瘤大小、病理分级和淋巴结转移有关。TUNEL检测结果显示，LIN28A蛋白表达阳性的乳腺癌组织中凋亡细胞率低于与癌旁组织（P < 0.05）。Kaplan-Meier生存分析显示，乳腺癌组织LIN28A蛋白表达阳性患者的生存率低于LIN28A蛋白表达阴性者（P < 0.05）。结论：RNA结合蛋白LIN28A可能通过EMT促进乳腺癌的进展和转移，并且LIN28A高表达可能影响乳腺癌患者的预后。

关键词: 浸润性乳腺癌, LIN28A, E-钙黏蛋白, 波形蛋白, 上皮间质转化, 凋亡

Abstract:

OBJECTIVE: To investigate the expression of RNA-binding proteins LIN28A,epithelial-mesenchymal transition (EMT) -related proteins E-cadherin and vimentin in invasive breast cancer and to understand their role in development of invasive breast cancer. METHODS: Quantitative real-time PCR (qPCR) and immunohistochemical staining were used to detect expressions of LIN28A,E-cadherin and vimentin mRNAs and proteins in invasive breast cancer tissues and the corresponding adjacent tissues. Relationships between mRNA and protein expressions of LIN28A,E-cadherin and vimentin were analyzed. TUNNEL staining was used to detect apoptosis of invasive breast cancer tissues with high LIN28A expressions. The relationship between LIN28A and EMT-related proteins and survival rate of breast cancer patients were statistically analyzed. RESULTS: qPCR results show that the relative expression level of LIN28A mRNA in breast cancer tissues were higher than adjacent tissues, negatively correlated with E-cadherin mRNA,and positively correlated with vimentin mRNA(P < 0.05). Immunohitochemistry staining results show that in breast cancer tissues,the expression rates of LIN28A,E-cadherin and vimentin protein were 60%,55% and 65%,respectively. The expression of LIN28A protein was negatively correlated with E-cadherin protein,positively correlated with vimentin protein. LIN28A expression was correlated with tumor size,histological grade and lymph node metastasis. TUNNEL assay show that high expression of LIN28A could decrease apoptosis in invasive breast cancer(P < 0.05). The survival rate of patients with positively expressed LIN28A was significantly lower than that of LIN28A negative patients (P < 0.05). CONCLUSION: The RNA-binding protein,LIN28A,may promote the occurrence,development and metastasis of breast cancer via EMT,while high expression of LIN28A may affect prognosis of patients with breast cancer.

Key words: invasive breast cancer, LIN28A, E-cadherin, vimentin, epithelial-mesenchymal transition, apoptosis

中图分类号:

R730.2

李幼梅, 张思仪, 赵连梅, 单保恩, 张超. LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义[J]. 癌变·畸变·突变, 2017, 29(4): 304-308,312.

LI Youmei, ZHANG Siyi, ZHAO Lianmei, SHAN Baoen, ZHANG Chao. Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 304-308,312.

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LIN28A、E-cadherin和vimentin在浸润性乳腺癌中的表达及其临床意义

Expressions of LIN28A,E-cadherin and vimentin in invasive breast cancer

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