癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (1): 71-75.doi: 10.3969/j.issn.1004-616x.2018.01.014

• 检测研究 • 上一篇    下一篇

三氯生的体外遗传毒性评价

曹易懿1, 奚晶1, 唐伟锋2, 尤馨悦1, 刘维映1, 栾洋1   

  1. 1. 上海交通大学医学院虹桥国际医学研究院/公共卫生学院, 上海 200025;
    2. 上海大学环境与化学工程学院, 环境污染与健康研究所, 上海 200444
  • 收稿日期:2017-10-31 修回日期:2017-12-25 出版日期:2018-01-30 发布日期:2018-01-30
  • 通讯作者: 栾洋,E-mail:yluan@sjtu.edu.cn E-mail:yluan@sjtu.edu.cn
  • 作者简介:曹易懿,E-mail:yycao@shsmu.edu.cn。
  • 基金资助:
    上海市卫生和计划生育委员会青年项目(20144Y0100)

Genotoxicity evaluation of triclosan in vitro

CAO Yiyi1, XI Jing1, TANG Weifeng2, YOU Xinyue1, LIU Weiying1, LUAN Yang1   

  1. 1. Hongqiao International Institute of Medicine/Faculty of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai 200025;
    2. Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
  • Received:2017-10-31 Revised:2017-12-25 Online:2018-01-30 Published:2018-01-30

摘要: 目的:应用体外碱性彗星试验、体外胞质分裂阻滞微核细胞组学试验和细菌回复突变试验(Ames试验)评价三氯生(TCS)的体外遗传毒性。方法:采用TK6人淋巴母细胞进行体外彗星试验和体外微核试验,共设定5个剂量(3.5、8.8、17.5、26.3和35 μmol/L)组。同时,应用鼠伤寒沙门氏菌TA98、TA100和DNA修复酶缺陷型YG7108(Ogt-/Ada-)菌株对TCS进行Ames试验,共设定8个剂量(0.000 5、0.001 67、0.005、0.016 7、0.05、0.167、0.5和1.67 μg/皿)组。结果:与对照组比较,彗星试验结果显示,TCS在各个剂量下均能引起TK6细胞的尾长、尾部DNA强度和尾矩的增加,差异均具有统计学意义(P均 < 0.01),且尾部DNA强度呈现剂量效应(r=0.943,P=0.017);微核试验表明,TCS未引起TK6细胞微核率升高(P > 0.05);Ames试验结果表明TCS未引起TA98、TA100和YG7108菌株的回复突变菌落数增加(P > 0.05)。结论:TCS主要引起细胞DNA损伤,但未对TK6细胞造成染色体损伤,对Ames试验菌株不具有致突变作用。

关键词: 三氯生, 彗星试验, 微核试验, Ames试验, 遗传毒性

Abstract: OBJECTIVE: Genotoxicity of triclosan was evaluated in TK6 and bacterial cells by alkaline comet assay,cytokinesis-block micronucleus (CBMN) test and bacterial reverse mutation test (Ames test). METHODS: Comet assay and CBMN test were performed by TK6 cell,and 5 doses of TCS were set as 3.5,8.8,17.5,26.3 and 35 μmol/L. Three Salmonella typhimurium tester strains TA98,TA100 and YG7108 (DNA repair deficient strain,Ogt-/Ada-) were employed to perform Ames test,and 8 doses of TCS were set at 0.000 5,0.001 67,0.005,0.016 7,0.05,0.167,0.5 and 1.67 μg/plate. RESULTS: The results of comet assay showed that TCS caused DNA damage with a significant increase in comets tail length,tail DNA intensity and tail moment (P < 0.01),and have a statistical correlation with tail DNA intensity (r=0.943,P=0.017). The CBMN test indicated that TCS did not induce increased frequency of micronucleus (P > 0.05) and no consistent increase in numbers of revertant colonies of three strains was seen in Ames test (P > 0.05). CONCLUSION: TCS mainly induced severe DNA injury to TK6 cell in a dose-dependent manner. TCS did not cause chromosome damage and also was not mutagenic to three Salmonella typhimurium strains.

Key words: triclosan, comet assay, micronucleus test, Ames test, genotoxicity

中图分类号: