癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (2): 136-139.doi: 10.3969/j.issn.1004-616x.2018.02.011

• 检测研究 • 上一篇    下一篇

农药哌虫啶的遗传毒性试验

夏莹, 付少华, 张寅静, 王薇   

  1. 湖北省疾病预防控制中心, 湖北省预防科学院, 湖北 武汉 430065
  • 收稿日期:2017-03-10 修回日期:2018-01-03 出版日期:2018-03-30 发布日期:2018-03-30
  • 作者简介:夏莹,E-mail:16222201@qq.com

Genetic toxicity test of a pesticide, Paichongding

XIA Ying, FU Shaohua, ZHANG Yinjing, WANG Wei   

  1. Hubei Provincial Center for Disease Control and Prevention, Hubei Academy of Preventive Medicine, Wuhan 430065, Hubei, China
  • Received:2017-03-10 Revised:2018-01-03 Online:2018-03-30 Published:2018-03-30

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摘要： 目的：采用体外CHO-K1细胞HGPRT基因突变试验和小鼠骨髓嗜多染红细胞微核试验，分析农药哌虫啶是否具有遗传毒性。方法：在培养的CHO-K1细胞中，分别加入含阳性物和不同浓度（625.0、312.5、156.3、78.1 μg/mL）哌虫啶的培养液进行染毒（+S9/-S9），并设阴性对照组。染毒后将细胞按低密度分种，进行突变体的选择及集落形成率的测定，同时计算细胞存活率。按每皿2×105个细胞接种，加入6-TG，同时另按每皿200个细胞接种，7 d后计算集落数、存活率和突变频率。小鼠骨髓嗜多染红细胞微核试验，分别设置高（2 500 mg/kg）、中（1 500 mg/kg）、低（625 mg/kg）3个哌虫啶剂量组，阳性对照组和阴性对照组，取股骨做骨髓涂片，观察1 000个嗜多染红细胞中出现微核的细胞数，计数200个嗜多染红细胞数（PCE），同时计数成熟红细胞数（NCE），并计算PCE占红细胞总数（PCE+NCE）的百分比。结果：与阴性对照组比较，哌虫啶625.0 μg/mL可明显降低CHO-K1细胞相对存活率，哌虫啶各剂量组细胞基因突变频率差异均无统计学意义（P > 0.05），阳性对照组细胞基因突变频率明显增加（P < 0.01）；哌虫啶各组未成熟红细胞占红细胞总数的比例均不少于阴性对照组的20%。与阴性对照组比较，阳性对照组雌雄小鼠微核率有显著性差异（P < 0.01），而哌虫啶各剂量组雌雄小鼠微核率差异均无显著性（P > 0.05）。结论：在本实验条件下，未发现哌虫啶的遗传毒性。

关键词: 哌虫啶, 遗传毒性, HGPRT基因突变实验, 微核试验

Abstract: OBJECTIVE: The in vitro CHO-K1 cells HGPRT gene mutation test and the mouse bone marrow micronucleus test were used to analysis the expression of genetic toxicity of a pesticide,Paichongding. METHODS: In CHO-K1 cells,the positive medium and different concentrations of Paichongding were added into the culture medium (+S9/-S9). After treatment of the cells at low density,mutant selection and colony formation were calculated at the same time with cell survival rates. The 2×105/dish was inoculated with 6-TG,at the same time,the other by the 200/plate were calculated after 7 d,colony number,survival rate and mutation frequency. Mouse bone marrow cell micronucleus test were conducted with high,medium and low Paichongding dose groups,positive control group and negative control group. Femoral bone marrow smears were observed:1 000 polychromatic erythrocytes micronucleus in polychromatic cells and 200 polychromatic erythrocytes (PCE) and the number of mature red blood cells (NCE),red blood cell count percentage calculation PCE. RESULTS: Compared with the control group,the highest dose of Paichongding significantly reduced the relative survival rate of CHO-K1 cells. Paichongding cell gene mutation frequency in each dose group had no significant difference (P > 0.05) while the positive control group (cell gene mutation frequency) had significant difference (P < 0.01). From the Paichongding treated mice,the immature red blood cells accounted for the proportion of the total number of red blood cells was not less than 20% of the negative control group. Compared with the negative control group,there was significant difference between male and female mice in positive control group (P < 0.01),while there was no significant difference in the micronucleus rate of male and female mice in each dose group (P > 0.05). CONCLUSION: Under the experimental conditions,no genetic toxicity was found with Paichongding。

Key words: Paichongding, genetic toxicity, HGPRT gene mutation test, micronucleus test

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