RNF2和PCNA在食管鳞状细胞癌中的表达及临床意义

doi:10.3969/j.issn.1004-616x.2018.04.002

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (4): 254-257.doi: 10.3969/j.issn.1004-616x.2018.04.002

RNF2和PCNA在食管鳞状细胞癌中的表达及临床意义

杨兴肖¹, 邹乃祎², 李巧芳², 张雪原², 宋姮², 刘志坤², 马鸣³, 单保恩⁴, 祝淑钗²

1. 河北医科大学第四医院感染科, 河北石家庄 050011;
2. 河北医科大学第四医院放疗科, 河北石家庄 050011;
3. 河北医科大学第四医院检验科, 河北石家庄 050011;
4. 河北医科大学第四医院科研中心, 河北石家庄 050011

收稿日期:2017-11-14 修回日期:2018-06-14 出版日期:2018-07-30 发布日期:2018-07-30
通讯作者: 祝淑钗,E-mail:sczhu1965@163.com E-mail:sczhu1965@163.com
作者简介:杨兴肖,E-mail:yangxingxiao2007@sina.com
基金资助:
国家自然科学基金资助项目（81372416）；河北省自然科学基金资助项目（H2017206170）；河北省医学科学研究所资助项目（20160183，20170154，20180483）

Significance in expression of RNF2 and PCNA proteins in esophageal squamous cancer cells

YANG Xingxiao¹, ZOU Naiyi², LI Qiaofang², ZHANG Xueyuan², SONG Heng², LIU Zhikun², MA Ming³, SHAN Baoen⁴, ZHU Shuchai²

1. Department of Infection, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011;
2. Department of Radiation Oncology, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011;
3. Department of Laboratory, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011;
4. Research Centre, the Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2017-11-14 Revised:2018-06-14 Online:2018-07-30 Published:2018-07-30

摘要/Abstract

摘要： 目的：探讨多梳基因家族成员环指蛋白2（RNF2）和增殖细胞核抗原（PCNA）蛋白在食管癌组织中的表达及其临床意义。方法：应用免疫组化法检测64例食管癌患者癌组织、30例癌旁正常组织中RNF2和PCNA蛋白的表达情况，分析其与临床病理指标及预后的关系。随访并分析RNF2阳性表达与食管癌患者术后5年存活率的关系。结果：RNF2和PCNA蛋白在肿瘤组织中均高表达，RNF2在癌组织和癌旁组织中的阳性表达率分别为54.69%（35/64）和30.00%（9/30），差异有统计学意义（χ²=5.000，P < 0.05）；PCNA在癌组织和癌旁组织中的阳性表达率分别为60.94%（39/64）和46.67%（14/30），差异有统计学意义（χ²=6.237，P < 0.05）；2种蛋白的阳性表达均与食管癌的肿瘤大小、TNM分期和淋巴结转移有关（P < 0.05），而与患者年龄、性别、分化程度无关（P > 0.05）；食管癌组织中PCNA蛋白表达与RNF2蛋白表达具有正相关关系（r=0.494，P < 0.01）。5年总生存期分析结果显示RNF2阳性表达者的生存时间更短（P < 0.01）。结论：RNF2和PCNA与食管癌的发生发展密切相关，且二者在食管癌发生发展中具有协同作用。

关键词: 食管癌, RNF2, PCNA, 免疫组织化学法

Abstract: OBJECTIVE: To evaluate expression of ring finger protein 2(RNF2) and proliferating cell nuclear antigen(PCNA) in tumor tissue of patients with esophageal squamous cancer cells and their clinial significance. METHODS: By using immunohistochemical method,the expression of RNF2 and PCNA in tumor tissues and the tissues adjacent to tumors were detected in 64 cases of esophageal squamous cell cancer and in 30 cases of tissues adjacent to tumors. Relationships between their expression,and clinical pathology and prognosis were analyzed. The relationship between positive expression of RNF2 and postoperative 5-year survival rates of patients with esophageal cancers was also explored. RESULTS: RNF2 and PCNA proteins showed high expression in the tumor tissues. The positive expression rate of RNF2 was 54.69%(35/64) in the tumor tissues and 30.00%(9/30) in the adjacent tissues. The difference was statistically significant (χ²=5.000,P < 0.05). The positive expression rate of PCNA was 60.94%(39/64) in the tumor tissues and 46.67%(14/30) in the adjacent tissues. The difference was statistically significant (χ²=6.237,P < 0.05). The expressions of RNF2 and PCNA were closely related to the tumor size,TNM stage and lymph node metastasis (P < 0.05). However,no correlations were observed between the expression of RNF2 and other clinical features such as patient age,gender,and histological grade (P > 0.05). Among the esophageal cancer group,PCNA protein expression was significantly and positively correlated with RNF2 protein expression (r=0.494,P < 0.01). In addition,the survival time of patients with RNF2 positive expression was shorter(P < 0.01). CONCLUSION: RNF2 and PCNA expressions were found to be closely related and they also showed synergistic effects in the development of esophageal cancer.

Key words: esophageal cancer, RNF2, PCNA, immunohistochemistry

中图分类号:

R730.2

杨兴肖, 邹乃祎, 李巧芳, 张雪原, 宋姮, 刘志坤, 马鸣, 单保恩, 祝淑钗. RNF2和PCNA在食管鳞状细胞癌中的表达及临床意义[J]. 癌变·畸变·突变, 2018, 30(4): 254-257.

YANG Xingxiao, ZOU Naiyi, LI Qiaofang, ZHANG Xueyuan, SONG Heng, LIU Zhikun, MA Ming, SHAN Baoen, ZHU Shuchai. Significance in expression of RNF2 and PCNA proteins in esophageal squamous cancer cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4): 254-257.

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RNF2和PCNA在食管鳞状细胞癌中的表达及临床意义

Significance in expression of RNF2 and PCNA proteins in esophageal squamous cancer cells

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