多溴联苯醚BDE-3、BDE-47和BDE-209的体外遗传毒性评价

doi:10.3969/j.issn.1004-616x.2020.01.003

摘要/Abstract

摘要： 目的：对3种代表性多溴联苯醚（PBDEs）BDE-3、BDE-47和BDE-209进行体外遗传毒性评价。方法：采用TK6人淋巴母细胞进行彗星试验、微核试验和TK基因突变试验，同时检测DNA损伤、染色体改变和基因突变3个遗传学终点。每种PBDEs均设定5个剂量组：BDE-3为60、90、120、180和240 μmol/L；BDE-47为60、120、180、200和240 μmol/L；BDE-209为24、40、120、180和240 μmol/L；同时设定溶媒DMSO为阴性对照组。结果：与阴性对照组比较，3种PBDEs在各个剂量下均未能引起TK6细胞彗星的尾长、尾部DNA百分数和尾矩的增加（P > 0.05），也均未引起TK6细胞微核率升高（P > 0.05）。TK基因突变试验显示，3种PBDEs均能引起TK基因突变频率升高，差异具有统计学意义（P < 0.05），并呈剂量依赖性升高趋势（相关系数[R_BDE-3²]=0.85，[R_BDE-47²]=0.85，[R_BDE-209²]=0.90；P < 0.05），但致突变作用BDE-47强于BDE-3和BDE-209。结论：多溴联苯醚BDE-3、BDE-47和BDE-209对TK6细胞具有致突变作用。

关键词: 多溴联苯醚, 彗星试验, 微核试验, TK基因突变试验, 遗传毒性

Abstract: OBJECTIVE: To evaluate in vitro genotoxicity of three representative Polybrominated Diphenyl Ethers (PBDEs):BDE-3,BDE-47 and BDE-209. METHODS: In vitro alkaline comet assay,in vitro cytokinesis-block micronucleus (CBMN) test and TK gene mutation assay were conducted using TK6 cells. These assays detect DNA damage,chromosome aberrations and gene mutation. Five doses of each PBDEs were used:60,90,120,180 and 240 μmol/L for BDE-3;60,120,180,200 and 240 μmol/L for BDE-47;and 24,40,120,180 and 240 μmol/L for BDE-209;while DMSO was used as negative control. RESULTS: Comparing with the negative control groups,exposure to the three PBDEs did not cause DNA damage,i.e. an increase in comets tail length,tail DNA intensity and tail moment (P > 0.05);and also did not increase the frequency of micronucleus (P > 0.05). However,the same exposures increased TK gene mutation frequencies significantly (P < 0.05) and in a dose-dependent manner ([R_BDE-3²]=0.85,[R_BDE-47²]=0.85,[R_BDE-209²]=0.90). The mutagenicity of BDE-47 was strongest. CONCLUSION: BDE-3,BDE-47 and BDE-209 were mutagenic to TK6 cell.

Key words: polybrominated diphenyl ethers, comet assay, micronucleus test, TK gene mutation assay, genotoxicity

中图分类号:

R994.6

曹易懿, 刘维映, 尤馨悦, 奚晶, 陈瑞雪, 张新宇, 栾洋. 多溴联苯醚BDE-3、BDE-47和BDE-209的体外遗传毒性评价[J]. 癌变·畸变·突变, 2020, 32(1): 15-20,28.

CAO Yiyi, LIU Weiying, YOU Xinyue, XI Jing, Chen Ruixue, ZHANG Xinyu, LUAN Yang. In vitro genotoxicity evaluation of BDE-3, BDE-47 and BDE-209[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 15-20,28.

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