降脂酮对棕榈酸诱导BRL-3A细胞胰岛素抵抗的保护作用

doi:10.3969/j.issn.1004-616x.2020.01.004

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (1): 21-28.doi: 10.3969/j.issn.1004-616x.2020.01.004

降脂酮对棕榈酸诱导BRL-3A细胞胰岛素抵抗的保护作用

金磊¹, 周珈右², 韩家诚², 管浩军², 王帅¹, 彭洁¹, 王欣¹, 海春旭¹, 李文丽¹

1. 空军军医大学预防医学系军事毒理学与防化医学教研室, 特殊作业环境危害评估与防治教育部重点实验室, 陕西省自由基生物学与医学重点实验室, 陕西西安 710032;
2. 空军军医大学基础医学院学员队, 陕西西安 710032

收稿日期:2019-05-24 修回日期:2020-01-02 出版日期:2020-01-31 发布日期:2020-02-05
通讯作者: 李文丽,E-mail:liwenli@fmmu.edu.cn E-mail:liwenli@fmmu.edu.cn
作者简介:金磊,E-mail:kingleer001@163.com;周珈右,E-mail:907165991@qq.com;韩家诚,E-mail:2419481437@qq.com。
基金资助:
国家自然科学基金项目（31170807，21677176，31770915）；陕西省创新人才提升计划项目（2017KJXX-42）；陕西省自然科学基础研究计划项目（2018JM7107）

Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells

JIN Lei¹, ZHOU Jiayou², HAN Jiacheng², GUAN Haojun², WANG Shuai¹, PENG Jie¹, WANG Xin¹, HAI Chunxu¹, LI Wenli¹

1. Department of Military Toxicology and Chemical Prevention Medicine, Key Lab of Hazard Assessment and Control in Special Operational Environment of the Ministry of Education, Shaanxi Key Lab of Free Radical Biology and Medicine, Air Force Medical University, Xi'an, 710032;
2. Cadet Team, School of Basic Medical, Air Force Medical University, Xi'an, 710032, Shaanxi, China

Received:2019-05-24 Revised:2020-01-02 Online:2020-01-31 Published:2020-02-05

摘要/Abstract

摘要： 目的：通过研究降脂酮（JZT）对胰岛素抵抗（IR）细胞模型糖脂代谢及氧化应激水平的影响，探讨降脂酮对胰岛素抵抗的保护作用及其机制。方法：将BRL-3A大鼠肝细胞系分为对照组、棕榈酸组及降脂酮组。对照组加入无血清RPMI-1640培养液；PA组100 μmol/L PA培养12 h；JZT组用JZT预处理后，换用100 μmol/L PA培养12 h。采用MTT法检测细胞生存率；试剂盒检测TG、TC、HDL-C、LDL-C水平；GOD-POD法检测基础葡萄糖消耗和胰岛素刺激葡萄糖消耗变化；DCFH-DA染色法检测细胞ROS水平，试剂盒检测CAT活性、T-AOC、MDA及GSH含量。结果：25 mg/mL降脂酮降低BRL-3A细胞的生存率（P < 0.05）；降脂酮可以提高IR模型细胞基础葡萄糖摄取量和HDL-C水平，降低TG、TC、LDL-C水平（P < 0.05）；降脂酮可以减少IR模型细胞ROS水平，降低MDA含量，增加CAT活性及T-AOC（P < 0.05）。结论：降脂酮对IR模型细胞具有一定的保护作用，其机制可能是与降脂酮降低IR模型细胞氧化应激损伤，并改善糖脂代谢有关。

关键词: 胰岛素抵抗, 活性氧, 氧化应激, 2型糖尿病, 棕榈酸, 降脂酮

Abstract: OBJECTIVE: To investigate effects of Jiangzhitong (JZT) on glucolipid metabolism and oxidative stress level in insulin resistance (IR) cells,and to study the protective effect of JZT on IR. METHODS: The rat hepatic cell line,BRL-3A,was sub-divided to the control,Palmitic Acid and JZT groups. Cell survival rates were determined using MTT. Levels of lucose consumption and insulin-stimulated glucose consumption were detected using GOD-POD method. ROS levels were detected using the DCFH-DA method. Triglycerides,total cholesterol,high density lipoprotein cholesterol,low-density lipoprotein cholesterol levels,catalase activity,T-AOC,MDA content and GSH content were detected using different kits. RESULTS: Exposure to 25 mg/mL JZT significantly decreased the survival rates of cell. JZT exposure increased levels of basic glucose consumption,high density lipoprotein cholesterol,while decreased triglycerides,total cholesterol,low-density lipoprotein cholesterol in IR cells. JZT also significantly decreased levels of ROS and malondialdehyde,while increased catalase activity and total antioxidant capacity in IR cells. CONCLUSION: JZT demonstrated protective effects on IR cells which could be due to reduced oxidative stress and improved glucolipid metabolism.

Key words: insulin resistance, reactive oxygen species, oxidative stress, type 2 diabetes mellitus, palmitic acid, Jiangzhitong

中图分类号:

R285.5

金磊, 周珈右, 韩家诚, 管浩军, 王帅, 彭洁, 王欣, 海春旭, 李文丽. 降脂酮对棕榈酸诱导BRL-3A细胞胰岛素抵抗的保护作用[J]. 癌变·畸变·突变, 2020, 32(1): 21-28.

JIN Lei, ZHOU Jiayou, HAN Jiacheng, GUAN Haojun, WANG Shuai, PENG Jie, WANG Xin, HAI Chunxu, LI Wenli. Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 21-28.

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降脂酮对棕榈酸诱导BRL-3A细胞胰岛素抵抗的保护作用

Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells

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