癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (3): 177-181.doi: 10.3969/j.issn.1004-616x.2020.03.004

• 论著 • 上一篇    下一篇

MiR-429及Bmi-1 mRNA在弥漫性大B细胞淋巴瘤中的表达及其临床意义

栾亦然1, 刘丽源1, 皎龙1, 汪月玲1, 白函瑜2, 邹乃祎2, 马鸣1   

  1. 1. 河北医科大学第四医院检验科, 河北 石家庄 050011;
    2. 河北医科大学第四医院科研中心, 河北 石家庄 050011
  • 收稿日期:2020-03-12 修回日期:2020-04-28 出版日期:2020-05-31 发布日期:2020-06-03
  • 通讯作者: 马鸣,E-mail:maming19830419@163.com E-mail:maming19830419@163.com
  • 作者简介:栾亦然,E-mail:abbycharlotte@foxmail.com。
  • 基金资助:
    国家自然科学基金(81703073);河北省自然科学基金(H2018206115);河北医科大学大学生创新性实验计划项目

Clinical significance of miR-429 and Bmi-1 mRNA expression in diffuse large B-cell lymphoma

LUAN Yiran1, LIU Liyuan1, JIAO Long1, WANG Yueling1, BAI Hanyu2, ZOU Naiyi2, MA Ming1   

  1. 1. Department of Laboratory, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011;
    2. Research Centre, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
  • Received:2020-03-12 Revised:2020-04-28 Online:2020-05-31 Published:2020-06-03

摘要: 目的:研究miR-429和Bmi-1 mRNA在弥漫性大B细胞淋巴瘤(DLBCL)组织中的表达及其临床意义。方法:选取自2014年1月—2015年12月河北医科大学第四医院的45例DLBCL患者瘤组织和28例正常对照人群淋巴结组织,用实时荧光定量反转录PCR(RT-qPCR)技术检测组织中miR-429及Bmi-1 mRNA的表达水平,并分析二者之间的关系及其与患者临床病理指标之间的关系。结果:DLBCL组织中miR-429表达水平较正常淋巴结组织明显降低,而Bmi-1 mRNA的表达水平明显升高(P < 0.01)。miR-429低表达组和Bmi-1 mRNA高表达组患者临床分期、骨髓浸润程度、Ki-67阳性率和IPI积分均分别高于miR-429高表达组和Bmi-1 mRNA低表达组(P < 0.05)。结论:DLBCL组织中miR-429及Bmi-1 mRNA的表达水平与患者病情进展和预后密切相关。组织中低表达miR-429及高表达Bmi-1 mRNA可能是患者临床预后较差的预测指标。

关键词: miR-429, 弥漫性大B细胞淋巴瘤, 临床意义, Bmi-1

Abstract: OBJECTIVE: To study the clinical significance of miR-429 and Bmi-1 mRNA in Diffuse large B-cell lymphoma (DLBCL) tissues. METHODS: Samples of tumor tissues were collected from 45 cases of the DLBCL patients from January 2014 to December 2015,which were compared with 28 normal lymph node tissues. Expressions of miR-429 and Bmi-1 mRNA in tumor tissues and normal lymph node tissues were detected by Reverse transcription quantitative polymerase chain reaction (RT-qPCR). Their relationships with the clinical features of the patients were analysed. RESULTS: The tumor tissues showed significantly decreased expression of miR-429 and increased expression of Bmi-1 mRNA,compared with the normal lymph node tissues. DLBCL patients with low expression of miR-429 and high expression of Bmi-1 tend to have later clinical stages,more prone to bone marrow metastasis,higher Ki-67 positive rate and higher IPI score than patients with high expression of miR-429 and low expression of Bmi-1 mRNA (P < 0.05). CONCLUSION: The results show that the expression levels of miR-429 and Bmi-1 in DLBCL tissues were closely related to the progression and prognosis of the lymphomas. Lower miR-429 expression and higher Bmi-1 mRNA expression in tumor tissues may be the predictors of poorer prognosis.

Key words: miR-429, DLBCL, clinical significance, Bmi-1

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