血清谷胱甘肽过氧化物酶与晚期非小细胞肺癌患者临床病理指标及预后的相关性

doi:10.3969/j.issn.1004-616x.2021.04.008

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (4): 286-290,295.doi: 10.3969/j.issn.1004-616x.2021.04.008

血清谷胱甘肽过氧化物酶与晚期非小细胞肺癌患者临床病理指标及预后的相关性

刘晓霞¹, 李卫霞¹, 李志加², 苏镇军², 段晓辉², 韩维文¹, 王云晓², 贺晓磊², 吕林林², 贾金海³

1. 河北工程大学医学院, 河北邯郸 056038;
2. 河北工程大学附属医院, 河北邯郸 056002;
3. 河北医科大学, 河北石家庄 050017

收稿日期:2021-02-24 修回日期:2021-06-10 出版日期:2021-07-30 发布日期:2021-07-29
通讯作者: 贾金海,E-mail:hbydmzb@163.com E-mail:hbydmzb@163.com
作者简介:刘晓霞,E-mail:givesunny@163.com。
基金资助:
河北省科技支撑项目（18277703D）；河北省医学适用技术跟踪项目（GZ2020100）

Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer

LIU Xiaoxia¹, LI Weixia¹, LI Zhijia², SU Zhenjun², DUAN Xiaohui², HAN Weiwen¹, WANG Yunxiao², HE Xiaolei², Lu Linlin², JIA Jinhai³

1. Medical College of Hebei University of Engineering, Handan 056038;
2. Affiliated Hospital of Hebei University of Engineering, Handan 056002;
3. Hebei Medical University, Shijiazhuang 050017, Hebei, China

Received:2021-02-24 Revised:2021-06-10 Online:2021-07-30 Published:2021-07-29

摘要/Abstract

摘要： 目的：探讨血清谷胱甘肽过氧化物酶（GSH-PX）活性与晚期非小细胞肺癌（NSCLC）患者临床病理指标、放化疗联合治疗的疗效及预后的相关性。方法：收集174例2018年1—12月于河北工程大学附属医院肿瘤科初次确诊、未经抗癌治疗的晚期NSCLC患者作为研究对象，以80例健康体检者为对照，患者于放化疗联合治疗前采集外周血，分离血清。采用基因表达谱数据动态分析（GEPIA）数据库分析GSH-PX的编码基因GPX3在不同肿瘤组织中的表达情况；5'，5-二硫硝基苯甲酸（DTNB）法检测GSH-PX活性，比较放化疗联合治疗前晚期NSCLC患者和对照组血清GSH-PX活性，并分析血清GSH-PX活性与晚期NSCLC临床病理指标、放化疗联合治疗疗效及预后的相关性。结果：GEPIA数据库分析显示33类肿瘤组织中，21类肿瘤组织的GPX3 mRNA表达低于正常组织，其中包括肺鳞癌和肺腺癌（P<0.05）；DTNB法检测结果显示晚期NSCLC患者血清GSH-PX活性显著低于健康对照组（P<0.05）；与年龄、性别、病理类型无明显相关（均为P>0.05），与吸烟史和肿瘤大小呈负相关（r=-0.165、-0.267，均为P<0.05），与卡氏（KPS）评分和病理分级呈正相关（r=0.295、0.228，均为P<0.05）；治疗有效患者和无效患者血清GSH-PX活性的差异无统计学意义（P>0.05），但GSH-PX高活性组患者总生存期显著高于低活性组（P<0.05），且呈正相关（r=0.238，P=0.000）。结论：晚期NSCLC患者血清GSH-PX活性降低，与患者吸烟史、KPS评分、肿瘤大小、病理分级和总生存期显著相关。GSH-PX可能参与了NSCLC的发生发展，可作为评估预后的指标。

关键词: 非小细胞肺癌, 谷胱甘肽过氧化物酶, 临床病理指标, 疗效, 总生存期, 预后

Abstract: OBJECTIVE: To investigate correlations between glutathione peroxidase (GSH-PX) in serum and clinicopathological indicators,curative effect of combined radio-with chemotherapy and prognosis of advanced non-small cell lung cancers (NSCLC). METHODS: In 2018,174 newly diagnosed advanced NSCLC patients (before anticancer therapy) from the Department of Oncology,Affiliated Hospital of Hebei University of Engineering and 80 healthy controls were recruited for our investigation. Expressions of GPX3 in different tumors were analyzed via the data of on-line. Activities of GSH-PX in serum from peripheral blood samples were detected via 5',5-dithio-bis-nitrobenzoic acid (DTNB). Activities of GSH-PX were compared between the advanced NSCLC and the control groups. In addition,relationships between activities of GSH-PX and clinicopathological indicators,curative effect of combined radio-with chemotherapy and prognosis of advanced non-small cell lung cancer were analyzed. RESULTS: Data from the gene expression profiling interactive analysis (GEPIA) show that expressions of GPX3 in 33 kinds of tumors (lung squamous cell carcinoma and lung adenocarcinoma) were significantly lower than that in the controls (P<0.05). Results from DTNB show that GSH-PX activities in serums of patients were significantly lower than that in the controls (P<0.05) and were irrelevant to the age,gender and pathological patterns of patients (P>0.05). However GSH-PX activities were negatively related to smoking histories and tumor sizes (r=-0.295、0.228,all P<0.05),and positively related to KPS scores and pathological grades (r=0.295、0.228,P all <0.05). There was no difference in GSH-PX activities between patients with effective and failed treatments (P>0.05). Overall survivals in the high activities group was higher than those in the low activities group (P<0.05),and they were positively related (r=0.238,P=0.000). CONCLUSION: GSH-PX activities in serum of patients with advanced NSCLC were significantly reduced,and the activities were related to smoking histories,KPS scores,tumor sizes,pathological grades and overall survivals. Therefore,GSH-PX activities were involved in the development and prognosis of NSCLC.

Key words: non-small cell lung cancer, glutathione peroxidase, clinicopathologic indicators, crutive effect, overall survival, prognosis

中图分类号:

R734.2

刘晓霞, 李卫霞, 李志加, 苏镇军, 段晓辉, 韩维文, 王云晓, 贺晓磊, 吕林林, 贾金海. 血清谷胱甘肽过氧化物酶与晚期非小细胞肺癌患者临床病理指标及预后的相关性[J]. 癌变·畸变·突变, 2021, 33(4): 286-290,295.

LIU Xiaoxia, LI Weixia, LI Zhijia, SU Zhenjun, DUAN Xiaohui, HAN Weiwen, WANG Yunxiao, HE Xiaolei, Lu Linlin, JIA Jinhai. Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 286-290,295.

参考文献

[1] 孙可欣, 郑荣寿, 张思维, 等. 2015年中国分地区恶性肿瘤发病和死亡分析[J]. 中国肿瘤, 2019, 28(1):1-11.
[2] 汤小朋, 陈磊, 熊康宁, 等. 硒蛋白-哺乳动物谷胱甘肽过氧化物酶家族研究进展[J]. 生命的化学, 2019, 39(6):1076-1081.
[3] 李博. MCP-1、PAPP-A、GSH-PX与冠心病合并糖尿病的相关性研究[D]. 承德:承德医学院, 2018.
[4] 王文斐, 金晓琴. PIH患者治疗前后血浆MDA、SOD和GSH-PX水平的变化[J]. 放射免疫学杂志, 2013, 26(5):698-699.
[5] VERA M, TORRAMADE-MOIX S, MARTIN-RODRIGUEZ S, et al. Antioxidant and anti-inflammatory strategies based on the potentiation of glutathione peroxidase activity prevent endothelial dysfunction in chronic kidney disease[J]. Cell Physiol Biochem, 2018, 51(3):1287-1300.
[6] 叶冠伦, 郑伟明, 罗卓章. Graves病及桥本病患者血硒、GSH-px及甲状腺素水平分析[J]. 海南医学, 2016, 27(14):2292-2294.
[7] YI Z H, JIANG L, ZHAO L, et al. Glutathione peroxidase 3(GPX3) suppresses the growth of melanoma cells through reactive oxygen species (ROS)-dependent stabilization of hypoxia-inducible factor 1-α and 2-Α[J]. J Cell Biochem, 2019, 120(11):19124-19136.
[8] PELOSOF L, YERRAM S, ARMSTRONG T, et al. GPX3 promoter methylation predicts platinum sensitivity in colorectal cancer[J]. Epigenetics, 2017, 12(7):540-550.
[9] 李新亚, 何国伟, 刘晓程. 术前血浆谷胱甘肽过氧化物酶-6水平与冠状动脉旁路移植术后新发房颤相关性研究[J]. 蚌埠医学院学报, 2018, 43(12):1581-1584.
[10] KANGARI P, ZARNOOSHEH FARAHANY T, GOLCHIN A, et al. Enzymatic antioxidant and lipid peroxidation evaluation in the newly diagnosed breast cancer patients in Iran[J]. Asian Pac J Cancer Prev, 2018, 19(12):3511-3515.
[11] 刘静, 吴建平, 寇耀, 等. SOD、GSH-PX、MDA在胃癌患者血清中的表达及与临床分期的关系[J]. 癌症进展, 2019, 17(13):1576-1578.
[12] HO S P, CHAN-YEUNG M, CHOW K K, et al. Antioxidant enzyme activities in healthy Chinese adults:influence of age, gender and smoking[J]. Respirology, 2005, 10(3):305-309.
[13] 毛静, 程思益, 王雪茹, 等. 平风降气汤对慢性阻塞性肺疾病稳定期的疗效及对血清SOD、GSH-PX的影响[J]. 中华中医药杂志, 2020, 35(4):2185-2188.
[14] 肖兴军, 苏文娜, 李利, 等. NGB、SOD及MDA对TIA预后意义[J]. 脑与神经疾病杂志, 2020, 28(3):181-184.

[1]	彭友彬, 林浩, 黎明, 黄海花. TPX2在非肌层浸润性膀胱癌中的表达及其临床意义[J]. 癌变·畸变·突变, 2022, 34(1): 30-34.
[2]	焦文鹏, 侯琳, 崔妹娟, 焦文静, 马鸣, 张金艳. miR-93-5p和miR-106b-5p在酒精相关性肝癌中的表达及其临床意义[J]. 癌变·畸变·突变, 2021, 33(6): 430-434.
[3]	高晓斌, 武雪亮, 王胜杰, 孙光源, 王文静, 梁峰, 赵轶峰, 刘振显, 张迎春. TRIM59在结直肠癌中的表达及其与临床病理指标的关系[J]. 癌变·畸变·突变, 2021, 33(6): 446-450.
[4]	焦文静, 张金艳, 马鸣, 郭秀娟, 冯军花, 邵俊国, 焦文鹏. 异黏蛋白与叉头框蛋白M1在肝细胞癌中的表达及其临床意义[J]. 癌变·畸变·突变, 2021, 33(5): 349-353,359.
[5]	张世民, 赵瑞君. 蛋白酪氨酸磷酸酶受体J在乳腺癌组织中的表达及其与临床预后的相关性[J]. 癌变·畸变·突变, 2021, 33(4): 276-279,285.
[6]	王熙凯, 孟庆贺, 高燕鲁. miR-223-3p通过调控ECT2诱导非小细胞肺癌细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(3): 187-192.
[7]	张玉铃, 吴俊东, 潘东岳, 陈春发. 乳腺癌新辅助化疗前后Ki67变化对疗效及预后的意义[J]. 癌变·畸变·突变, 2021, 33(3): 230-235,245.
[8]	焦文静, 郭秀娟, 马鸣, 邵俊国, 张金艳. 老年局部晚期食管癌同步放化疗与单纯放疗的疗效比较[J]. 癌变·畸变·突变, 2021, 33(2): 124-128.
[9]	赵子慧, 王绪华. 非小细胞肺癌伴随诊断的研究进展[J]. 癌变·畸变·突变, 2021, 33(1): 66-71,76.
[10]	李玮玮, 朱莹. 数据挖掘分析MYB家族在乳腺癌中的表达及其预后评估价值[J]. 癌变·畸变·突变, 2020, 32(6): 414-422.
[11]	石海林, 何天基, 刘峰, 蔡孟会, 葛波. 基于TCGA数据库分析肿瘤突变负荷在肌层浸润性膀胱癌预后评估中的价值[J]. 癌变·畸变·突变, 2020, 32(6): 430-437,443.
[12]	丁雅雯, 焦文静, 王芃堉, 鹿刚, 马鸣. 多发性骨髓瘤患者浆细胞免疫表型特点及其临床意义研究[J]. 癌变·畸变·突变, 2020, 32(6): 464-468,489.
[13]	黎梓蕊, 许键, 李红文, 张鹏. 口腔癌相关microRNA的研究进展[J]. 癌变·畸变·突变, 2020, 32(6): 481-484.
[14]	刘晓霞, 李卫霞, 苏镇军, 贾金海, 段晓辉, 李志加, 贺晓磊, 吕林林, 刘睿, 王云晓. 晚期非小细胞肺癌病理特征及放化疗联合治疗疗效与患者血清超氧化物歧化酶的关系[J]. 癌变·畸变·突变, 2020, 32(5): 350-354.
[15]	邵俊国, 焦文静, 王雪晓, 张金艳, 马鸣. miR-429和Bmi-1 mRNA在肺癌组织中的表达及其临床意义[J]. 癌变·畸变·突变, 2020, 32(5): 369-373.

血清谷胱甘肽过氧化物酶与晚期非小细胞肺癌患者临床病理指标及预后的相关性

Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价