癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (5): 365-369.doi: 10.3969/j.issn.1004-616x.2021.05.009

• 论著 • 上一篇    下一篇

齐墩果酸对酒精诱导的大鼠胃壁氧化损伤的保护作用

李宏伟1,2, 孔德钦1, 于卫华1, 吴昊1, 王钊1, 刘瑞1, 海春旭1, 王欣1, 刘江正1, 李文丽1   

  1. 1. 空军军医大学军事预防医学系军事毒理学与防化医学教研室, 陕西省自由基生物学与医学重点实验室, 教育部特殊作业环境危害评估与防治重点实验室, 陕西 西安 710032;
    2. 63710部队医院, 山西 忻州 036301
  • 收稿日期:2021-07-22 修回日期:2021-08-25 出版日期:2021-09-30 发布日期:2021-10-09
  • 通讯作者: 刘江正,E-mail:liu.jiangzheng@163.com;李文丽,E-mail:liwenli@fmmu.edu.cn E-mail:liu.jiangzheng@163.com;liwenli@fmmu.edu.cn
  • 作者简介:李宏伟,E-mail:1970172769@qq.com。

Protective effect of oleanolic acid co-treatment on alcohol-induced oxidative damage in gastric mucosa of rats

LI Hongwei1,2, KONG Deqin1, YU Weihua1, WU Hao1, WANG Zhao1, LIU Rui1, HAI Chunxu1, WANG Xin1, LIU Jiangzheng1, LI Wenli1   

  1. 1. Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, Key Lab of Hazard Assessment and Control in Special Operational Environment of Ministry of Education, Air Force Medical University, Xi'an 710032, Shaanxi;
    2. 63710 Military Hospital, Xinzhou 036301, Shanxi, China
  • Received:2021-07-22 Revised:2021-08-25 Online:2021-09-30 Published:2021-10-09

摘要: 目的:研究化学单体齐墩果酸(OA)对酒精诱导的大鼠胃壁损伤的保护作用。方法:采用随机数表法将24只SD大鼠随机分为对照组(等热量葡萄糖溶液)、酒精暴露组(灌胃给予4 g/kg酒精构建酒精性胃壁损伤模型)、OA干预组(灌胃给予溶解了10 mg/kgOA的酒精溶液进行干预),共3组,每组8只,持续30 d。观察OA对酒精诱导的大鼠胃壁损伤是否具有保护作用。检测胃壁大体改变和HE病理变化;胃壁组织丙二醛(MDA)和氧化型谷胱甘肽(GSSG)含量;还原型谷胱甘肽(GSH)含量及GSH/GSSG比值,抗氧化酶转录因子Nrf-2的mRNA和蛋白表达;TNF-α、IL-6的mRNA和蛋白表达水平。结果:与对照组相比,4 g/kg的酒精暴露30 d可以成功诱导大鼠胃壁氧化损伤和炎症反应。与酒精暴露组大鼠相比,OA干预组的胃壁病理损伤程度减轻,胃壁组织MDA、GSSG含量减少(P < 0.05),GSH含量和GSH/GSSG比值增加(P < 0.05),Nrf-2的mRNA和蛋白表达水平显著升高(P < 0.05),同时TNF-α、IL-6的mRNA和蛋白表达降低(P < 0.05)。结论:OA可以通过抗氧化和抗炎作用发挥对酒精诱导大鼠胃壁损伤的保护作用。

关键词: 齐墩果酸, 酒精, 氧化应激, 炎症

Abstract: OBJECTIVE: To study the protective effect of oleanolic acid (OA) on alcohol-induced gastric mucosal injury in rats. METHODS: Using the random number table method, 24 SD rats were randomly divided into 3 groups of 8 rats per group:control (isocaloric glucose solution),alcohol exposure (gavage 4 g/kg alcohol to construct alcoholic gastric wall injury model),OA intervention (perfusion to the stomach with an alcohol solution of 10 mg/kg OA). All treatments lasted 30 days. Rats were evaluated for general changes of the stomach wall and pathological changes of HE; content of malondialdehyde (MDA) and oxidized glutathione (GSSG) in the stomach wall; content of reduced glutathione (GSH) and ratio of GSH/GSSG,antioxidant enzyme transcription Factor Nrf-2 mRNA and protein expression; and TNF-α, IL-6 mRNA and protein expression levels. RESULTS: Compared with the control group,4 g/kg alcohol exposure for 30 days successfully induced oxidative damage and inflammatory reactions of the gastric wall in rats. Compared with the alcohol-exposed rats, pathological damage to the stomach wall in the OA intervention group was reduced, contents of MDA and GSSG in the stomach wall tissue were decreased (P < 0.05),GSH contents and the GSH/GSSG ratios were increased (P < 0.05),and Nrf-2 was increased. mRNA and protein expression levels were increased significantly (P< 0.05),while mRNA and protein expressions of TNF-α and IL-6 were decreased (P < 0.05). CONCLUSION: OA played a protective role on alcohol-induced gastric wall injury in rats through its antioxidant and antiinflammatory effects.

Key words: oleanolic acid, alcohol, oxidative stress, inflammation

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