N-乙酰半胱氨酸对纳米二氧化钛诱发细胞遗传损伤的保护作用

doi:10.3969/j.issn.1004-616x.2022.02.003

摘要/Abstract

摘要： 目的：探讨抗氧化剂N-乙酰半胱氨酸(NAC)对纳米二氧化钛(TiO₂-NPs)诱发人正常肝细胞L-02和肺细胞HBE遗传损伤的保护作用。方法：分别将对数生长期的L-02和HBE细胞分为3组，即正常培养的对照组、TiO₂-NPs(80μg/mL)暴露组、TiO₂-NPs(80μg/mL)与NAC(20μmol/L)联合暴露组；各组细胞处理72h后，采用H₂O₂试剂盒检测细胞内H₂O₂浓度；实时荧光定量(qPCR)法检测L-02、HBE细胞端粒长度(TL)的改变；胞质分裂阻断微核细胞组试验(CBMN-Cyt)评估受试细胞的染色体不稳定性(CIN)及细胞死亡率。结果：与对照组相比，TiO₂-NPs暴露72h后，可诱导L-02和HBE细胞内H₂O₂浓度显著升高(P<0.05)，致使L-02和HBE细胞TL缩短、CIN增加、细胞死亡率增加(P<0.05)；当TiO₂-NPs与NAC联合暴露72h后时，两种受试细胞的H₂O₂水平和CIN仍较对照组显著升高(P<0.05)，而TL和细胞死亡率与对照组间的差异均无统计学意义(P>0.05)；与单独暴露TiO₂-NPs组相比，联合暴露时两种受试细胞的TL显著增加(P<0.05)，且CIN及细胞死亡率显著降低(P<0.05)。结论：抗氧化剂NAC可抑制TiO₂-NPs诱发的人肝细胞L-02和肺细胞HBE的TL缩短和细胞死亡，维护染色体稳定，对细胞有一定保护作用。

关键词: N-乙酰半胱氨酸, 纳米二氧化钛, 端粒, 遗传损伤, 保护作用

Abstract: OBJECTIVE： To investigate protective effects of N-acetylcysteine (NAC) on genotocicity induced by titanium dioxide nanoparticles (TiO₂-NPs) in human normal hepatocytes L- 02 and human normal lung epithelial cells HBE. METHODS：L-02 and HBE cells grown in log phase were divided into 3 groups： control group (normal culture)，TiO₂-NPs exposed group (80 μg/mL)，and TiO₂-NPs (80 μg/mL) and NAC (20 μmol/L) co-exposed group. After the cells in each group were exposed for 72 h，H₂O₂ kit was used to detect the intracellular H₂O₂ level; telomere length (TL) of L-02 and HBE cells in each group was measured by realtime quantitative PCR; chromosomal instability (CIN) and cell death indexes were evaluated by cytokinesisblock micronucleus cytome assay (CBMN-Cyt). RESULTS： Compared with the control group， TiO₂-NPs exposed for 72 h significantly increased intracellular H₂O₂ levels in L-02 and HBE cells (P<0.05)，resulting in shortened TL，increased CIN and cell death (P<0.05). When co-exposed with TiO₂-NPs and NAC for 72 h， the H₂O₂ levels and CIN among the two types cells were significantly higher than those of the control group (P< 0.05)，while TL and cell death had no significant differences compared with the control group (P>0.05). When compared with the TiO₂-NPs group，increased TL (P<0.05)，decreased CIN，and decreased cell death rates were found in TiO₂-NPs and NAC co-exposed group (both P<0.05). CONCLUSION： The antioxidant NAC strongly reduced the telomere shortening and cell death induced by TiO₂-NPs in L- 02 and HBE cells， showing the effects of protecting cells under the stress of TiO₂-NPs.

Key words: N-acetylcysteine, titanium dioxide nanoparticles, telomere, genetic damage, protective effect

中图分类号:

Q355

王晗, 周滔, 徐云东, 郭锡汉, 倪娟, 汪旭. N-乙酰半胱氨酸对纳米二氧化钛诱发细胞遗传损伤的保护作用[J]. 癌变·畸变·突变, 2022, 34(2): 93-97.

WANG Han, ZHOU Tao, XU Yundong, GUO Xihan, NI Juan, WANG Xu. Protective effects of N-acetylcysteine on genotoxicity induced by titanium dioxide nanoparticles in vitro[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(2): 93-97.

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