放射性皮肤损伤中细胞干性的转变

doi:10.3969/j.issn.1004-616x.2026.02.004

摘要/Abstract

摘要： 目的： 研究放射性皮肤损伤中细胞干性的变化。方法： 体外细胞试验中，分别采用0、5、10和20 Gy组X射线对人永生化角质形成细胞HaCaT进行一次性照射，通过细胞划痕法、Transwell法检测照射后24和48 h细胞的迁移能力，用Western blot法检测HaCaT细胞照射后24和48 h干性相关蛋白KLF4、c-myc和LGR5的表达。在动物试验中，对SD大鼠右后肢皮肤进行20 Gy X射线照射，在照后第15和35天用Western blot法检测照射部位皮肤细胞中干性相关SOX2、Nanog、KLF4、c-myc、LGR5蛋白的表达，同时设未照射对照组(0 Gy)，每组4只大鼠。结果： 在体外细胞试验中，与对照组(0 Gy)相比，照射48 h后，细胞划痕法显示5、10和20 Gy X射线照射后细胞迁移能力均明显降低(P<0.05)，而Transwell实验结果显示细胞的迁移能力显著增强(P<0.01),10 Gy时仍保持一定迁移能力；在照射后24 h，不同剂量照射组HaCaT细胞中KLF4、c-myc和LGR5蛋白表达水平均显著降低(P<0.01)，而在照射后48 h,KLF4、c-myc和LGR5蛋白表达水平均显著升高(P<0.01)。在动物试验中，与对照组(0 Gy)相比，20 Gy X射线照射大鼠皮肤第15天后，干性相关SOX2、c-myc和LGR5蛋白表达水平升高(均为P<0.01)；照射第35天后SOX2、Nanog、LGR5蛋白表达水平显著降低(P<0.05或P<0.01)。结论： 在放射性皮肤损伤中，细胞干性转变能力是动态变化的，早期可能抑制修复，中期可能促进再生，但长期干性转变能力可能逐渐降低。

关键词: 电离辐射, 皮肤损伤, 干性基因, 修复

Abstract: OBJECTIVE: To investigate changes in cellular stemness transition in radiation-induced skin injury in cell culture. METHODS: Human immortalized keratinocytes(Ha Ca T) were exposed to X-ray at 0,5,10,and 20 Gy. Cell migration ability at 24 h and 48 h post-irradiation was assessed using the scratch wound healing assay and transwell migration assay. Western blot was used to detect the expression of stemness-related proteins KLF4,c-myc and LGR5 in Ha Ca T cells at 24 h and 48 h after irradiation. In the animal experiments,the skin of the right hind limb of SD rats was irradiated with a single dose of 20 Gy X-ray. At 15 and 35 days post-irradiation, western blot was performed to detect the expression of stemness-related proteins SOX2,Nanog, KLF4, c-myc, and LGR5 in the irradiated skin cells. A non-irradiated control group(0 Gy) was included, with 4 rats per group. RESULTS: Compared with the control group(0 Gy), the in vitro scratch wound healing assay at 48 h post-irradiation showed that cell migration ability was significantly reduced in the5, 10, and 20 Gy groups(P<0.05). In contrast, the transwell assay demonstrated significantly enhanced migration ability in all irradiated groups(P<0.01), with the 10 Gy group still retaining a certain level of migration capacity. At 24 h post-irradiation,KLF4,c-myc and LGR5 protein expression levels in Ha Ca T cells were significantly decreased in all irradiated dose groups(P<0.01). At 48 h post-irradiation,KLF4,c-myc and LGR5 protein expression levels were significantly increased(P<0.01). In the animal experiments,compared with the control group(0 Gy), at 15 days after 20 Gy X-ray irradiation, expression levels of stemness-related proteins SOX2, c-Myc, and LGR5 in rat skin were significantly elevated(all P<0.01). At 35 days postirradiation, expression levels of SOX2, Nanog, and LGR5 were significantly decreased(P<0.05 or P<0.01).CONCLUSION: In radiation-induced skin injury, cellular stemness transition capacity expressed dynamic changes which might cause inhibition of repair,promotion of regeneration with gradual decline in the long term.

Key words: ionizing radiation, skin injury, stemness genes, repair

中图分类号:

R994.6

关子易, 余南希, 程雪艳, 马丽萍, 高玲. 放射性皮肤损伤中细胞干性的转变[J]. 癌变·畸变·突变, 2026, 38(2): 108-112,118.

GUAN Ziyi, YU Nanxi, CHENG Xueyan, MA Liping, GAO Ling. Cellular stemness transition in radiation-induced skin injury in vitro and in vivo[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2026, 38(2): 108-112,118.

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