DNA依赖性蛋白激酶催化亚基在非同源末端连接修复中作用的研究进展

doi:10.3969/j.issn.1004-616x.2022.06.014

摘要/Abstract

摘要： DNA的精确复制对维持基因组稳定性具有重要作用，而DNA双链断裂(DSB)损伤可能诱导细胞凋亡和染色质重排，在肿瘤的发生发展过程中发挥作用。DNA依赖性蛋白激酶催化亚基(DNA-PKcs)是磷脂酰肌醇3-激酶样蛋白激酶(PIKK)家族中相对分子质量最大及表达量最高的丝氨酸/苏氨酸蛋白激酶。与Ku70/80结合后，DNA-PKcs在离子辐射诱导DSB后的非同源末端连接(NHEJ)有效修复过程中，发挥非常重要的作用。DNA-PKcs或其他NHEJ修复因子的缺失会导致辐射敏感性和DSB的未修复，以及V(D)J重组缺陷和免疫缺陷。本文主要对DNA-PKcs的结构，在DSB位点的募集与激活，在NHEJ修复中的作用过程及其与疾病的关系进行总结与展望，希望能为DNA-PKcs的深入研究及临床应用提供理论基础。

关键词: DNA依赖性蛋白激酶催化亚基, DNA双链断裂, DNA修复, 非同源末端连接, 肿瘤

中图分类号:

Q291

张天枫, 赵乐天, 廖明星, 李仁燕. DNA依赖性蛋白激酶催化亚基在非同源末端连接修复中作用的研究进展[J]. 癌变·畸变·突变, 2022, 34(6): 476-480.

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