纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究

doi:10.3969/j.issn.1004-616x.2017.05.010

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (5): 374-378.doi: 10.3969/j.issn.1004-616x.2017.05.010

纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究

崔胜金, 陈泽衍, 曹朝鹏, 黄荣, 刘青, 周义文

南方医科大学深圳医院检验科, 广东深圳 518101

收稿日期:2016-10-08 修回日期:2017-09-15 出版日期:2017-09-30 发布日期:2017-09-30
通讯作者: 周义文,E-mail:yiwenzhou21@aliyun.com E-mail:yiwenzhou21@aliyun.com
作者简介:崔胜金,E-mail:shirenyirui@163.com。

Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats

CUI Shengjin, CHEN Zeyan, CAO Zhaopeng, HUANG Rong, LIU Qing, ZHOU Yiwen

Department of Clinical Laboratory, Shenzhen Hospital of Southern Medical University, Shenzhen 518101, Guangdong, China

Received:2016-10-08 Revised:2017-09-15 Online:2017-09-30 Published:2017-09-30

摘要/Abstract

摘要： 目的：利用蛋白质组学技术研究纳米二氧化硅颗粒对SD雄性大鼠生殖系统的损伤机制，为深入了解纳米材料的毒性效应及机制提供科学依据。方法：采用鼻腔滴入纳米二氧化硅分散液方式对SD雄性大鼠进行染毒，设置为纳米二氧化硅组；同时经鼻腔滴入生理盐水设置为生理盐水对照组。两组于4周后断颈处死并取睾丸组织。利用荧光差异双向电泳（2D-DIGE）联合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）技术筛选并鉴定出差异表达蛋白，Western blot验证差异蛋白。结果：共筛选并鉴定出9个差异表达的蛋白，分别是GRP78、HSP7C、KNT1、HSP72、ALBU、STIP1、PDIA1、ALDR和GDIR1；并经Western blot验证了PDIA1和HSC70表达水平变化与2D-DIGE实验结果一致。结论：本研究发现纳米二氧化硅作用后大鼠睾丸组织中凋亡相关蛋白PDIA1和HSC70发生异常变化，初步阐明了二氧化硅染毒导致大鼠生殖毒性的细胞凋亡分子机制，为预防纳米二氧化硅毒性奠定基础。

关键词: 纳米二氧化硅, 睾丸, 生殖毒性, 荧光差异双向电泳

Abstract: OBJECTIVE: To study the adverse effect of nano-SiO₂ particles on reproductive system in male SD rats. METHODS: Male rats were treated with nano-SiO₂ particles or saline and then executed 4 weeks later. Testes were isolated for the extraction of protein. 2D-DIGE coupled with MALDI-TOF-MS was used to identify nano-SiO₂ induced abnormal expression of proteins. Two proteins were verified by Western blot analysis. RESULTS: Nano-SiO₂ induced abnormal expression of 9 proteins in rat testes (GRP78,HSP7C,KNT1,HSP72,ALBU,STIP1,PDIA1,ALDR and GDIR1). PDIA1 and HSP7C were validated by Western blot analysis,and the results were accordance with DIGE analysis. CONCLUSION: Our findings partially supported our early observation regarding seminiferous tubule dysgenesis in rat. In addition,this study revealed technological advancement in reproductive toxicology of SiO₂.

Key words: nano-SiO₂, testis, reproductive toxicology, 2D-DIGE

中图分类号:

R994.3

崔胜金, 陈泽衍, 曹朝鹏, 黄荣, 刘青, 周义文. 纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究[J]. 癌变·畸变·突变, 2017, 29(5): 374-378.

CUI Shengjin, CHEN Zeyan, CAO Zhaopeng, HUANG Rong, LIU Qing, ZHOU Yiwen. Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 374-378.

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纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究

Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats

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纳米二氧化硅经鼻黏膜染毒对大鼠睾丸毒性的机制研究

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Mechanisms for induction of reproductive toxicity by nano-SiO₂ particles in SD male rats