›› 2011, Vol. 23 ›› Issue (2): 119-122.doi: 10.3969/j.issn.1004-616x.2011.02.009

• 论著 • 上一篇    下一篇

电离辐射对胃癌细胞增殖和周期的影响

高 玲1,2，李峰生3，陈肖华2，刘青杰1,*   

  1. 1． 中国疾病预防控制中心辐射防护与核医学安全所，北京 100088； 2． 军事医学科学院放射与辐射医学研究所，北京 100850； 3． 第二炮兵总医院，北京 100088
  • 收稿日期:2010-10-20 修回日期:2010-11-20 出版日期:2011-03-30 发布日期:2011-03-30
  • 通讯作者: 刘青杰

Effects of ionizing radiation on the proliferation and cell cycle of gastric adenocarcinoma cells

GAO Ling1,2, LI Feng-sheng3, CHEN Xiao-hua2, LIU Qing-jie1,*   

  1. 1. National Institute for Radiological Protection，China CDC, Beijing 100088; 2. Institution of Radiate Medicine, Academy of Military Medical Sciences, Beijing 100850; 3. General Hospital of Second Artillery, Beijing 100088, China
  • Received:2010-10-20 Revised:2010-11-20 Online:2011-03-30 Published:2011-03-30
  • Contact: LIU Qing-jie

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摘要： 研究电离辐射对体外胃腺癌细胞增殖、凋亡及周期的影响。 方法： 60Coγ 射线单次照射胃腺癌BGC-823细胞，照射剂量分别为0、2、4 Gy，利用MTT和克隆形成率实验检测各剂量组BGC-823的增殖能力；同时利用Annexin-V/PI双染法检测各剂量组BGC-823细胞的凋亡；用PI单染测定各剂量组BGC-823的细胞周期。 结果： 与0 Gy组相比，2、4 Gy 60Coγ 射线照射可显著抑制胃腺癌BGC-823细胞的增殖(P＜0.05)，诱导BGC-823细胞在照射后依次出现S期和G2/M期阻滞。4 Gy 60Coγ射线照射后48 h，凋亡细胞比例显著高于对照组(P＜0.05)。结论：60Coγ射线照射可抑制胃腺癌细胞BGC-823增殖、诱导胃癌细胞出现周期阻滞和细胞凋亡。

关键词: 胃癌, 辐射, 增殖, 凋亡, 细胞周期

Abstract: To study the effects of ionizing radiation on the proliferation, apoptosis and cell cycle of gastric adenocarcinoma cells in vitro. METHODS: After gastric adenocarcinoma BGC-823 cells were irradiated by 60Coγ-rays with a single dose of 0, 2 or 4 Gy, the proliferation ability of BGC-823 cells in every dose group was evaluated by MTT and cloning efficiency assay. Apoptosis and cell cycle were further investigated by flow cytometry with Annexin-V/PI double staining and PI staining, respectively. RESULTS: Compared with the 0 Gy group, proliferation of gastric adenocarcinoma cells was inhibited significantly after doses of 2 and 4 Gy γ-rays irradiation (P<0.05). Furthermore, the cells were arrested in S then G2/M phases after γ-rays irradiation of 2 and 4 Gy. Finally, compared with 0 Gy group, the percentage of apoptotic cells in 4 Gy dose group increased significantly 48 h after irradiation (P<0.05). CONCLUSION: Radiation treatment of gastric adenocarcinoma BGC-823 cells with 60Coγ-rays could inhibit cell proliferation, induce cell cycle arrest and promote apoptosis.

Key words: gastric adenocarcinoma, radiation, proliferation, apoptosis, cell cycle

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