›› 2012, Vol. 24 ›› Issue (3): 195-198.doi: 10.3969/j.issn.1004-616x.2011.03.007

• 论著 • 上一篇    下一篇

维生素E琥珀酸酯通过内质网应激诱导胃癌SGC-7901细胞凋亡的研究

黄晓莉,赵 艳,张志宏,张旭光,王晓琳,贾 莉,吴 坤   

  1. 1. 哈尔滨医科大学营养与食品卫生教研室，黑龙江 哈尔滨 150081；2. 哈尔滨医科大学环境卫生学教研室，黑龙江 哈尔滨 150001
  • 收稿日期:2011-09-02 修回日期:2011-12-06 出版日期:2012-05-30 发布日期:2012-05-30
  • 通讯作者: 吴 坤

Vitamin E succinate-induced apoptosis of SGC-7901 cells via endoplasmic reticulum stress

HUANG Xiao-li，ZHAO Yan，ZHANG Zhi-hong，ZHANG Xu-guang，WANG Xiao-lin1，JIA Li ，WU Kun   

  1. 1. Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin 150081; 2. Environmental Hygiene Department, Harbin Medical University, Harbin 150001, Heilongjiang, China
  • Received:2011-09-02 Revised:2011-12-06 Online:2012-05-30 Published:2012-05-30
  • Contact: WU Kun

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摘要： 目的： 探讨内质网应激在维生素E琥珀酸酯 (vitamin E succinate，VES)诱导人胃癌SGC-7901细胞凋亡过程中的作用。方法：分别采用Western blotting 和RT-PCR检测VES不同剂量 (0、5、10和20 μg/ml)处理SGC-7901细胞24 h和20 μg/ml VES处理SGC-7901细胞不同时间对内质网应激标志物葡萄糖调节蛋白 (glucose regulative protein，GRP) GRP78、GRP94和内质网应激凋亡途径关键因子C/EBP同源蛋白 (C/EBP-homologous protein，CHOP)的蛋白和mRNA表达的影响；进一步采用RNA瞬时干扰阻断CHOP的表达后用20 μg/ml VES作用SGC-7901细胞24 h，采用DAPI染色法观察细胞凋亡的改变。结果：20 μg/ml VES组在mRNA水平上均诱导GRP78、GRP94和CHOP的表达增加；在蛋白水平上20 μg/ml VES显著增加GRP78和CHOP的表达，GRP94表达先降低，而后又升高；阻断CHOP后VES诱导的细胞凋亡由46.3%降低为27.9% (P<0.05)。结论：内质网应激可能参与了VES诱导的SGC-7901细胞凋亡。

关键词: 内质网应激, 维生素E琥珀酸酯, 细胞凋亡

Abstract: OBJECTIVE: To investigate the roles of endoplasmic reticulum stress (ERS) in vitamin E succinate (VES)-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS：SGC-7901 cells were treated with VES at 5，10 and 20 μg/ml and at 3 μg/ml tunicamycin (TM) for 24 h or at 20 μg/ml for up to 24 h. Glucose regulative protein 78 (GRP78)，GRP94 and C/EBP-homologous protein (CHOP) were explored using Western blotting and RT-PCR. When the cells were transfected with CHOP siRNA，apoptosis was assessed by DAPI staining. RESULTS：The mRNA levels of GRP78，GRP94，CHOP and protein levels of GRP78，CHOP increased in 20 μg/ml VES-treated cells. GRP94 protein levels were decreased at 6 h，12 h and 18 h，then increased again and reached the top level at 24 h．Downregulation of the CHOP mRNA reversed VES-induced apoptosis (P<0.05). CONCLUSION： Endoplasmic reticulum stress might be involved in VES-induced apoptosis in human gastric cancer SGC-7901 cells.

Key words: endoplasmic reticulum stress, vitamin E succinate, apoptosis

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